Park, C. H.; Amare, M.; Savin, M. A.; Hoogstraten, B.
Author Affiliation :
Division of Clinical Oncology, Dep. Medicine, Univ. Kansas Medical Center, 39th and Rainbow Blvd., Kansas City, Kans, 66103, USA.
The suppressive effect of L-ascorbic acid on the growth of bone marrow cells from patients with acute nonlymphocytic leucaemia was studied by a modified agar culture method, with daily feeding to allow the growth of leucaemic cell colonies. In 7 of 28 patients the numbers of leucaemic cell colonies grown in culture were reduced to 21% of control by the addition of L-ascorbic acid (0.3 mM) to the culture medium. Glutathione did not suppress leucaemic cell colonies although it has a similar oxidation-reduction potential to that of L-ascorbic acid. The addition of L-ascorbic acid reduced the pH of the medium. However, a comparable reduction of pH by the addition of HCl did not suppress leucaemic cell colonies. In simultaneous cultures for leucaemic and normal marrow cells, the leucaemic cell colony was suppressed with a concentration of L-ascorbic acid as low as 0.1 mM (a concentration achievable in vivo), but normal myeloid colonies were not suppressed until the concentration of L-ascorbic acid was extremely high (1 mM).
Record Number :
Language of text :
Language of summary :
Indexing terms for this abstract:
Organism descriptor(s) :
ascorbic acid, bone marrow cells, LEUKAEMIA, neoplasms
blood cancer, cancers, growth suppression of leucaemic cells in vitro by ascorbic acid, leucaemia, leukemia, vitamin C
Broader term(s) :
Homo, Hominidae, primates, mammals, vertebrates, Chordata, animals, eukaryotes