Guern, N.; Gawer, M.; Chervin, D.; Mazliak, P.
Author Affiliation :
Laboratoire de physiologie cellulaire (UA CNRS 1180), Université P. et M. Curie, 4, place Jussieu, 75252 Paris, Cedex 05, France.
Plant Science (Limerick)
Cells grown in vitro as suspension cultures slowly accumulated exogenous choline during the log phase of growth independently of their sensitivity or resistance to inhibitory levels of choline chloride. Cells from resistant lines repeatedly subcultured in the presence of choline (10 or 25 msmallcap˜M) displayed an ability to limit the intracellular accumulation of choline and the associated variation of osmotic potential. On the contrary, cells from the sensitive line actively absorbed and metabolized exogenous choline when supplied at 10-25 msmallcap˜M. At the stationary phase of growth, the internal concentration of free choline in sensitive cells was markedly increased as well as the osmotic pressure of cell fluids. After a few generations, cell divisions stopped and mortality rapidly increased.
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Organism descriptor(s) :
Nicotiana, Nicotiana tabacum, plants
Biotechnology, Cell cultures, choline, stimulant plants, tissue culture, Tobacco, toxic substances
poisons, stimulant crops
Broader term(s) :
Solanaceae, Solanales, eudicots, angiosperms, Spermatophyta, plants, eukaryotes, Nicotiana