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  • Comparison between tobacco cell lines either resistant or sensitive (wild type) to exogenous choline: intracellular accumulation of free choline.

    Author(s) : Guern, N.Gawer, M.Chervin, D.Mazliak, P.

    Author Affiliation : Laboratoire de physiologie cellulaire (UA CNRS 1180), Université P. et M. Curie, 4, place Jussieu, 75252 Paris, Cedex 05, France.

    Journal article : Plant Science (Limerick) 1990 Vol.71 No.2 pp.251-259 ref.24

    Abstract : Cells grown in vitro as suspension cultures slowly accumulated exogenous choline during the log phase of growth independently of their sensitivity or resistance to inhibitory levels of choline chloride. Cells from resistant lines repeatedly subcultured in the presence of choline (10 or 25 msmallcap˜M) displayed an ability to limit the intracellular accumulation of choline and the associated variation of osmotic potential. On the contrary, cells from the sensitive line actively absorbed and metabolized exogenous choline when supplied at 10-25 msmallcap˜M. At the stationary phase of growth, the internal concentration of free choline in sensitive cells was markedly increased as well as the osmotic pressure of cell fluids. After a few generations, cell divisions stopped and mortality rapidly increased.

    ISSN : 0168-9452

    DOI : 10.1016/0168-9452(90)90015-G

    Record Number : 19901617822

    Language of text : English

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Nicotiana, Nicotiana tabacum, plants

    Descriptor(s) : Biotechnology, Cell cultures, choline, stimulant plants, tissue culture, Tobacco, toxic substances

    Identifier(s) : poisons, stimulant crops

    Broader term(s) : Solanaceae, Solanales, eudicots, angiosperms, Spermatophyta, plants, eukaryotes, Nicotiana