Singh, D. K.; Varshney, B. C.; Raghav, P. R. S.; Thakur, M.
Author Affiliation :
Animal Disease Research Laboratory, National Dairy Development Board, Anand 388001, Gujarat, India.
Indian Veterinary Journal
A cell line of Theileria annulata schizont-infected bovine lymphoid cells was established and grown in vitro for 453 passages (2038 days). After 12, 27, 48, 100 and 150 passages the culture-derived cells were test-inoculated into crossbred calves which were subsequently challenged with Ground Up Tick Supernates (GUTS) prepared from T. annulata-infected Hyalomma anatolicum anatolicum ticks. The virulence of the theilerial schizonts was reduced by passage. Calves receiving 5 × 106 schizont-infected lymphoid cells at the 150th passage (675 days) had minimum inoculation reactions and could withstand virulent challenge of T. annulata infective GUTS given on day 47 after inoculation. It was concluded that T. annulata schizont-infected bovine lymphoid cell cultres of the Anand isolate at the 150th passage could be used effectively for immunizing cattle against tropical theileriosis.
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Indexing terms for this abstract:
Organism descriptor(s) :
cattle, Hyalomma, Protozoa, Theileria, Theileria annulata
cattle diseases, cell culture vaccines, immunization, infections, parasites, parasitoses, pathogenicity, protozoal infections, theileriosis, tickborne diseases, virulence
immune sensitization, parasitic diseases, parasitic infestations, parasitosis, protozoal diseases, tick-borne diseases
Broader term(s) :
Bos, Bovidae, ruminants, Artiodactyla, mammals, vertebrates, Chordata, animals, eukaryotes, Ixodidae, Metastigmata, Acari, Arachnida, arthropods, invertebrates, Theileria, Theileriidae, Piroplasmorida, Apicomplexa, Protozoa