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  • Salivary amylase activity of the phlebotomine sand fly, Lutzomyia longipalpis.

    Author(s) : Ribeiro, J. M. C.Rowton, E. D.Charlab, R.

    Author Affiliation : Section of Medical Entomology, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Building 4, Room 126, 4 Center Drive, MSC-0425, NIH, Bethesda, MD 20892-0425, USA.

    Journal article : Insect Biochemistry and Molecular Biology 2000 Vol.30 No.4 pp.271-277 ref.28

    Abstract : Both male and female adult stages of the sand fly Lutzomyia longipalpis have detectable amylase activity in their salivary glands, as indicated by formation of p-nitrophenyl-α-D-maltoside from p-nitrophenyl-α-D-octoside and by hydrolysis of 4-nitrophenyl-α-D-maltoheptaoside-4,6,-O-ethylidene. No salivary α-glucosidase was detected. Amylase activity was also found in the crop and midgut of female flies, although in a smaller amount. Salivary amylase is significantly reduced from the salivary glands immediately after a blood meal, as is the case with salivary α-glucosidases in mosquitoes. Presence of salivary gland amylase in these sand flies, and absence of salivary α-glucosidase, indicates that in nature these insects may have a significant intake of carbohydrates in the form of starch, as suggested by their plant-feeding behaviour, previously demonstrated in related species under experimental conditions and in a Columbian coffee plantation.

    ISSN : 0965-1748

    DOI : 10.1016/S0965-1748(99)00119-8

    Record Number : 20000506149

    Language of text : English

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Diptera, Lutzomyia longipalpis, Phlebotominae, Psychodidae

    Descriptor(s) : alpha-glucosidase, amylases, carbohydrates, diet, digestion, midgut, saliva, salivary glands

    Identifier(s) : alpha-D-glucosidase, maltase, saccharides, salivary secretions

    Broader term(s) : insects, Hexapoda, arthropods, invertebrates, animals, eukaryotes, Lutzomyia, Phlebotominae, Psychodidae, Diptera