Ribeiro, J. M. C.; Rowton, E. D.; Charlab, R.
Author Affiliation :
Section of Medical Entomology, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Building 4, Room 126, 4 Center Drive, MSC-0425, NIH, Bethesda, MD 20892-0425, USA.
Insect Biochemistry and Molecular Biology
Both male and female adult stages of the sand fly Lutzomyia longipalpis have detectable amylase activity in their salivary glands, as indicated by formation of p-nitrophenyl-α-D-maltoside from p-nitrophenyl-α-D-octoside and by hydrolysis of 4-nitrophenyl-α-D-maltoheptaoside-4,6,-O-ethylidene. No salivary α-glucosidase was detected. Amylase activity was also found in the crop and midgut of female flies, although in a smaller amount. Salivary amylase is significantly reduced from the salivary glands immediately after a blood meal, as is the case with salivary α-glucosidases in mosquitoes. Presence of salivary gland amylase in these sand flies, and absence of salivary α-glucosidase, indicates that in nature these insects may have a significant intake of carbohydrates in the form of starch, as suggested by their plant-feeding behaviour, previously demonstrated in related species under experimental conditions and in a Columbian coffee plantation.
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Indexing terms for this abstract:
Organism descriptor(s) :
Diptera, Lutzomyia longipalpis, Phlebotominae, Psychodidae
alpha-glucosidase, amylases, carbohydrates, diet, digestion, midgut, saliva, salivary glands
alpha-D-glucosidase, maltase, saccharides, salivary secretions
Broader term(s) :
insects, Hexapoda, arthropods, invertebrates, animals, eukaryotes, Lutzomyia, Phlebotominae, Psychodidae, Diptera