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  • Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR.

    Author(s) : Wen WeiGangCui JunXiaSheng Lei

    Author Affiliation : Ningbo Entry-Exit Inspection and Quarantine Bureau, Ninbo 315012, Zhejiang Province, China.

    Author Email : wenwg@nbciq.gov.cn

    Journal article : Acta Phytophylacica Sinica 2007 Vol.34 No.1 pp.61-66 ref.10

    Abstract : Tobacco ringspot virustobacco ringspot virusSubject Category: Organism Names
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    (TRSV) and Tomato ringspot virustomato ringspot virusSubject Category: Organism Names
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    (ToRSV) are quarantine diseases. For the detection of these two viruses, we adopted Trizol for total RNA extraction and developed a semi-nested RT-PCR method with its specific primers, which was designed on the basis of the coding genes of TRSV and ToRSV coat protein, respectively. Products of TRSV by RT-PCR and semi-nested PCR were 359 and 206 bp, that of ToRSV were 340 and 219 bp. Sequencing of the semi-nested RT-PCR products of TRSV and ToRSV indicated that their identities with the TRSV and ToRSV coat protein genes were 88-97% and 96-100%, respectively. Further study showed that the detection limits of DAS-ELISA and RT-PCR methods were about the same, but 103 times lower than that of the semi-nested RT-PCR at least.

    ISSN : 0577-7518

    URL : http://www.wanfangdata.com.cn

    Record Number : 20073076549

    Publisher : Editorial Board of Acta Phytophylacica Sinica

    Location of publication : Beijing

    Country of publication : China

    Language of text : Chinese

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : ComovirinaecomovirinaeSubject Category: Organism Names
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    , NepovirusnepovirusSubject Category: Organism Names
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    , Tobacco ringspot virustobacco ringspot virusSubject Category: Organism Names
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    , Tomato ringspot virustomato ringspot virusSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    Descriptor(s) : coat proteinscoat proteinsSubject Category: Chemicals and Chemical Groups
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    , pathogenspathogensSubject Category: Organism Groups
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    , plant pathogensplant pathogensSubject Category: Organism Groups
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    , plant virusesplant virusesSubject Category: Organism Groups
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    , polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
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    , RNArnaSubject Category: Chemicals and Chemical Groups
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    Identifier(s) : capsid proteins, Comoviridae, PCR, phytopathogens, ribonucleic acid, viruses of plants

    Broader term(s) : virusesvirusesSubject Category: Organism Names
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    , ComovirinaecomovirinaeSubject Category: Organism Names
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    , SecoviridaesecoviridaeSubject Category: Organism Names
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    , PicornaviralespicornaviralesSubject Category: Organism Names
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    , positive-sense ssRNA Virusespositive-sense ssrna virusesSubject Category: Organism Names
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    , ssRNA Virusesssrna virusesSubject Category: Organism Names
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    , RNA Virusesrna virusesSubject Category: Organism Names
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    , NepovirusnepovirusSubject Category: Organism Names
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