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  • Comparison of different pretreatment strategies for enzymatic hydrolysis of wheat and barley straw.

    Author(s) : Rosgaard, L.Pedersen, S.Meyer, A. S.

    Author Affiliation : Novozymes A/S Krogshøjvej 36, Bagsvaerd, 2880, Denmark.

    Journal article : Applied Biochemistry and Biotechnology 2007 Vol.143 No.3 pp.284-296

    Abstract : In biomassbiomassSubject Category: Miscellaneous
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    -to-ethanol processes a physico-chemical pretreatment of the lignocellulosic biomass is a critical requirement for enhancing the accessibility of the cellulose substrate to enzymatic attack. This report evaluates the efficacy on barley and wheat strawwheat strawSubject Category: Commodities and Products
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    of three different pretreatment procedures: acid or water impregnation followed by steam explosion versus hot water extraction. The pretreatments were compared after enzyme treatment using a cellulasecellulaseSubject Category: Chemicals and Chemical Groups
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    enzyme system, Celluclast 1.5 L® from Trichoderma reesei, and a β-glucosidase, Novozyme 188 from Aspergillus nigeraspergillus nigerSubject Category: Organism Names
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    . Barley strawbarley strawSubject Category: Commodities and Products
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    generally produced higher glucose concentrations after enzymatic hydrolysishydrolysisSubject Category: Miscellaneous
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    than wheat straw. Acid or water impregnation followed by steam explosion of barley straw was the best pretreatment in terms of resulting glucose concentration in the liquid hydrolysate after enzymatic hydrolysis. When the glucose concentrations obtained after enzymatic hydrolyses were related to the potential glucose present in the pretreated residues, the highest yield, 48% (g g-1), was obtained with hot water extraction pretreatment of barley straw; this pretreatment also produced highest yields for wheat straw, producing a glucose yield of 39% (g g-1). Addition of extra enzyme (Celluclast 1.5 L®+Novozyme 188) during enzymatic hydrolysis resulted in the highest total glucose concentrations from barley straw, 32-39 g L-1, but the relative increases in glucose yields were higher on wheat straw than on barley straw. Maldi-TOF MS analyses of supernatants of pretreated barley and wheat straw samples subjected to acid and water impregnation, respectively, and steam explosion, revealed that the water impregnated + steam-exploded samples gave a wider range of pentose oligomers than the corresponding acid-impregnated samples.

    ISSN : 0273-2289

    DOI : 10.1007/s12010-007-8001-6

    Record Number : 20083000956

    Publisher : Humana Press

    Location of publication : Totowa

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Aspergillus nigeraspergillus nigerSubject Category: Organism Names
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    , Hordeum vulgarehordeum vulgareSubject Category: Organism Names
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    , Trichoderma longibrachiatumtrichoderma longibrachiatumSubject Category: Organism Names
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    , TriticumtriticumSubject Category: Organism Names
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    Descriptor(s) : barleybarleySubject Category: Commodities and Products
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    , barley strawbarley strawSubject Category: Commodities and Products
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    , biomassbiomassSubject Category: Miscellaneous
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    , cellulasecellulaseSubject Category: Chemicals and Chemical Groups
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , ethanolethanolSubject Category: Chemicals and Chemical Groups
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    , ethanol productionethanol productionSubject Category: Miscellaneous
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    , hydrolysishydrolysisSubject Category: Miscellaneous
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    , lignocelluloselignocelluloseSubject Category: Chemicals and Chemical Groups
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    , strawstrawSubject Category: Commodities and Products
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    , waste managementwaste managementSubject Category: Miscellaneous
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    , waste treatmentwaste treatmentSubject Category: Miscellaneous
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    , wheatwheatSubject Category: Commodities and Products
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    , wheat strawwheat strawSubject Category: Commodities and Products
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    Identifier(s) : ethyl alcohol, fungus, Hyphomycetes

    Broader term(s) : AspergillusaspergillusSubject Category: Organism Names
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    , TrichocomaceaetrichocomaceaeSubject Category: Organism Names
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    , EurotialeseurotialesSubject Category: Organism Names
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    , EurotiomyceteseurotiomycetesSubject Category: Organism Names
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    , PezizomycotinapezizomycotinaSubject Category: Organism Names
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    , AscomycotaascomycotaSubject Category: Organism Names
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    , fungifungiSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , HordeumhordeumSubject Category: Organism Names
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    , PoaceaepoaceaeSubject Category: Organism Names
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    , PoalespoalesSubject Category: Organism Names
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    , commelinidscommelinidsSubject Category: Organism Names
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    , monocotyledonsmonocotyledonsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , TrichodermatrichodermaSubject Category: Organism Names
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    , HypocreaceaehypocreaceaeSubject Category: Organism Names
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    , HypocrealeshypocrealesSubject Category: Organism Names
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    , SordariomycetessordariomycetesSubject Category: Organism Names
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