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  • Gas chromatography-mass spectrometry assay for determination of Nτ-methylhistamine concentration in canine urine specimens and fecal extracts.

    Author(s) : Ruaux, C. G.Wright, J. M.Steiner, J. M.Williams, D. A.

    Author Affiliation : Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843-4474, USA.

    Journal article : American Journal of Veterinary Research 2009 Vol.70 No.2 pp.167-171 ref.10

    Abstract : Objective - To develop and validate a gas chromatography-mass spectrometry (GC-MS) method for determination of Nτ-methylhistamine (NMH) concentration in canine urineurineSubject Category: Miscellaneous
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    and fecal extractsextractsSubject Category: Miscellaneous
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    and to assess urinary NMH concentrations in dogsdogsSubject Category: Organism Names
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    with mast cell neoplasia and fecal NMH concentrations in dogs with protein-losing enteropathy. Sample Population - Urine specimens were collected from 6 healthy dogs and 7 dogs with mast cell neoplasia. Fecal extracts were obtained from fecal specimens of 28 dogs with various severities of protein-losing enteropathy, as indicated by fecal concentration of α1-proteinase inhibitor. Procedures - NMH was extracted directly from urine, and fecal specimens were first extracted into 5 volumes of PBSS containing 1% newborn calf serum. Nτ-methylhistamine in specimens was quantified via stable isotope dilution GC-MS. The assay was validated via determination of percentage recovery of known amounts of NMH and interassay coefficients of variationvariationSubject Category: Properties
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    . Urinary excretionexcretionSubject Category: Miscellaneous
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    of NMH was evaluated by means of NMH-to-creatinine concentration ratiosratiosSubject Category: Properties
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    . Results - Recovery of NMH in urine and fecal extracts averaged 104.6% and 104.5%, respectively. Interassay coefficients of variation ranged from 5.4% to 11.7% in urine and 12.6% to 18.1% in fecal extracts. Urinary NMH excretion was significantly increased in dogs with mast cell neoplasia, compared with that in healthy dogs. No correlation was detected between severity of protein-losing enteropathy and fecal NMH concentration. Conclusions and Clinical Relevance - This method provided a sensitive, reproducible means of measuring NMH in canine urine and fecal extracts. High urinary NMH-to-creatinine concentration ratios in dogs with mast cell neoplasia are consistent with increased histaminehistamineSubject Category: Chemicals and Chemical Groups
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    releasereleaseSubject Category: Miscellaneous
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    in this disease.

    ISSN : 0002-9645

    DOI : 10.2460/ajvr.70.2.167

    Record Number : 20093101811

    Publisher : American Veterinary Medical Association

    Location of publication : Schaumburg

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : dogsdogsSubject Category: Organism Names
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    Descriptor(s) : analysisanalysisSubject Category: Techniques, Methodologies and Equipment
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    , assaysassaysSubject Category: Techniques, Methodologies and Equipment
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    , estimationestimationSubject Category: Techniques, Methodologies and Equipment
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    , excretionexcretionSubject Category: Miscellaneous
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    , extractsextractsSubject Category: Miscellaneous
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    , histaminehistamineSubject Category: Chemicals and Chemical Groups
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    , intestinal diseasesintestinal diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , isotopesisotopesSubject Category: Chemicals and Chemical Groups
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    , methodologymethodologySubject Category: Techniques, Methodologies and Equipment
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    , neoplasmsneoplasmsSubject Category: Diseases, Disorders, and Symptoms
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    , newborn animalsnewborn animalsSubject Category: Organism Groups
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    , ratiosratiosSubject Category: Properties
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    , releasereleaseSubject Category: Miscellaneous
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    , techniquestechniquesSubject Category: Techniques, Methodologies and Equipment
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    , tracerstracersSubject Category: Miscellaneous
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    , urineurineSubject Category: Miscellaneous
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    , variationvariationSubject Category: Properties
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    Identifier(s) : cancers, enteropathy, methods, urinary excretion

    Broader term(s) : CaniscanisSubject Category: Organism Names
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    , CanidaecanidaeSubject Category: Organism Names
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    , FissipedafissipedaSubject Category: Organism Names
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    , carnivorescarnivoresSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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