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  • Application of visible and near-infrared reflectance spectroscopy (Vis/NIRS) to determine carotenoid contents in banana (Musa spp.) fruit pulp.

    Author(s) : Davey, M. W.Saeys, W.Hof, E.Ramon, H.Swennen, R. L.Keulemans, J.

    Author Affiliation : Laboratory for Fruit Breeding and Biotechnology, Division of Crop Biotechnics, Katholieke Universiteit Leuven, De Croylaan 42, 3001 Leuven, Belgium.

    Author Email : Mark.Davey@biw.kuleuven.be

    Journal article : Journal of Agricultural and Food Chemistry 2009 Vol.57 No.5 pp.1742-1751 ref.34

    Abstract : The analysis of carotenoidscarotenoidsSubject Category: Chemicals and Chemical Groups
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    is complicated by the tendency of these compounds to react with radical species, leading to oxidative breakdown and isomerization during extraction. Therefore, protocols should be rapid and avoid unnecessary exposure to heat, acids, and so forth. Here, we evaluate the use of visible and near infrared reflectance spectroscopy (Vis/NIRS) to measure carotenoid contents in fruit from 28 MusamusaSubject Category: Organism Names
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    (banana and plantain) varieties. Carotenoid contents were first quantified using standardized RP-HPLChplcSubject Category: Techniques, Methodologies and Equipment
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    protocols, and these results were then used to develop algorithms to predict carotenoid contents from Vis/NIR spectra of the same samples. Cross-validation of the predictive algorithms across a genetically diverse group of varieties demonstrated that correlation coefficients between the HPLC measurements and the Vis/NIRS predictions varied from good for the total carotenoids and β-carotene fractions (r2cv, 0.84, 0.89) to reasonable for α-carotene and cis-carotenescarotenesSubject Category: Chemicals and Chemical Groups
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    (r2cv, 0.61, 0.66), but there was only a poor correlation (r2cv, 0.30) for the minor lutein component. Nonetheless, since 90% of the Musa carotenoids consist of only α- and β-carotene, results indicate that Vis/NIRS can be used for the high-throughput screening of fruit pulpfruit pulpSubject Category: Commodities and Products
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    samples for vitamin A nutritional content on the basis of their total carotenoids content.

    ISSN : 0021-8561

    DOI : 10.1021/jf803137d

    Record Number : 20093103042

    Publisher : American Chemical Society

    Location of publication : Washington

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : MusamusaSubject Category: Organism Names
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    Descriptor(s) : alpha-carotenealpha-caroteneSubject Category: Chemicals and Chemical Groups
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    , analytical methodsanalytical methodsSubject Category: Techniques, Methodologies and Equipment
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    , bananasbananasSubject Category: Commodities and Products
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    , beta-carotenebeta-caroteneSubject Category: Chemicals and Chemical Groups
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    , carotenescarotenesSubject Category: Chemicals and Chemical Groups
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    , carotenoidscarotenoidsSubject Category: Chemicals and Chemical Groups
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    , chemical compositionchemical compositionSubject Category: Properties
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    , cultivarscultivarsSubject Category: Organism Groups
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    , fruit pulpfruit pulpSubject Category: Commodities and Products
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    , HPLChplcSubject Category: Techniques, Methodologies and Equipment
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    , infrared spectroscopyinfrared spectroscopySubject Category: Techniques, Methodologies and Equipment
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    , near infrared spectroscopynear infrared spectroscopySubject Category: Techniques, Methodologies and Equipment
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    , retinolretinolSubject Category: Chemicals and Chemical Groups
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    , xanthophyllxanthophyllSubject Category: Chemicals and Chemical Groups
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    Identifier(s) : analytical techniques, axerophthol, cultivated varieties, high performance liquid chromatography, lutein, tetraterpenoids, vitamin A, vitamin A alcohol, vitamin A1

    Broader term(s) : MusaceaemusaceaeSubject Category: Organism Names
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    , ZingiberaleszingiberalesSubject Category: Organism Names
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    , commelinidscommelinidsSubject Category: Organism Names
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    , monocotyledonsmonocotyledonsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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