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  • Disruption of Arabidopsis CHY1 reveals an important role of metabolic status in plant cold stress signaling.

    Author(s) : Dong, C. H.Zolman, B. K.Bartel, B.Lee, B.Stevenson, B.Agarwal, M.Zhu, J. K.

    Author Affiliation : Department of Botany and Plant Science, 2150 Batchelor Hall, University of California at Riverside, Riverside, CA 92521, USA.

    Author Email : jian-kang.zhu@ucr.edu

    Editors : Bressan, R.Bohnert, H.Zhu, J. K.

    Journal article : Molecular Plant 2009 Vol.2 No.1 pp.59-72 ref.43

    Abstract : To study cold signaling, we screened for Arabidopsis mutants with altered cold-induced transcription of a firefly luciferase reporter gene driven by the CBF3 promoter (CBF3-LUC). One mutant, chy1-10, displayed reduced cold-induction of CBF3-LUC luminescence. RNA gel blot analysis revealed that expression of endogenous CBFs also was reduced in the chy1 mutant. chy1-10 mutant plants are more sensitive to freezing treatment than wild-type after cold acclimation. Both the wild-type and chy1 mutant plants are sensitive to darkness-induced starvation at warm temperatures, although chy1 plants are slightly more sensitive. This dark-sensitivity is suppressed by cold temperature in the wild-type but not in chy1. Constitutive CBF3 expression partially rescues the sensitivity of chy1-10 plants to dark treatment in the cold. The chy1 mutant accumulates higher levels of reactive oxygen species, and application of hydrogen peroxide can reduce cold-induction of CBF3-LUC in wild-type. Map-based cloning of the gene defective in the mutant revealed a nonsense mutation in CHY1, which encodes a peroxisomal β-hydroxyisobutyryl (HIBYL)-CoA hydrolase needed for valine catabolism and fatty acid β-oxidation. Our results suggest a role for peroxisomal metabolism in cold stress signaling, and plant tolerance to cold stress and darkness-induced starvation.

    ISSN : 1674-2052

    DOI : 10.1093/mp/ssn063

    URL : http://mplant.oxfordjournals.org/.../59

    Record Number : 20093242614

    Publisher : Oxford University Press

    Location of publication : Oxford

    Country of publication : UK

    Language of text : English

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Arabidopsis thaliana, Brassicales

    Descriptor(s) : cold tolerance, dark adaptation, gene expression, genes, genetic regulation, light relations, metabolism, mutants, mutations, signal transduction

    Identifier(s) : Capparales

    Broader term(s) : Arabidopsis, Brassicaceae, Brassicales, eudicots, angiosperms, Spermatophyta, plants, eukaryotes