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  • Development and testing of LAMP assay for diagnosis of Plasmodium falciparum malaria.

    Author(s) : Stridiron, A. K.

    Author Affiliation : Kapolei High School, Honolulu, Hawaii, USA.

    Journal article : Ethnicity & Disease 2009 Vol.19 No.2 suppl. 3 pp.S3-23-S3-24 ref.3

    Abstract : Malaria is one of the three major infectious diseases in the world. The malarial parasite, Plasmodium falciparum, causes an estimated two million deaths each year, primarily in children under the age of five years. Although the parasite has become resistant to some drugs, individuals can still be treated and cured if the disease is diagnosed early and accurately. The objective of this study was to develop a simple and rapid diagnostic test that can be used in malaria-endemic areas. Microscopic detection of the malarial parasites in human blood smears has long been used as a gold-standard for clinical diagnosis. Alternatively, nested polymerase chain reaction (PCR) has been used because of its higher sensitivity. Nevertheless, microscopy and nested PCR require extensive training, high-end resources and time. Recently, the loop-mediated isothermal amplification (LAMP) method has been developed to detect similar microbial infections. In my study, the LAMP assay was used to detect P. falciparum and the results were compared with traditional microscopy and nested PCR. A total of 25 blood samples from Cameroon and Thailand were obtained from consenting adults. Based on microscopy, 10 samples were negative and 15 were positive for P. falciparum. These 25 samples were coded and analyzed using nested PCR with purified DNA and using LAMP with both purified DNA and whole blood. The ultimate goal of this study was to evaluate the utility of the LAMP assay for diagnosis of malaria in developing countries.

    ISSN : 1049-510X

    URL : http://www.ishib.org/journal/19-2s3/e...

    Record Number : 20093243343

    Publisher : International Society on Hypertension in Blacks

    Location of publication : Georgia

    Country of publication : USA

    Language of text : English

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : man, Plasmodium, Plasmodium falciparum, Protozoa

    Descriptor(s) : assays, diagnosis, diagnostic techniques, human diseases, infections, malaria, parasites, parasitoses, polymerase chain reaction, protozoal infections, children

    Identifier(s) : loop-mediated isothermal amplification, parasitic diseases, parasitic infestations, parasitosis, PCR, protozoal diseases

    Broader term(s) : Homo, Hominidae, primates, mammals, vertebrates, Chordata, animals, eukaryotes, Plasmodiidae, Haemospororida, Apicomplexa, Protozoa, Plasmodium