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  • Tyrosine and phenylalanine are synthesized within the plastids in Arabidopsis.

    Author(s) : Rippert, P.Puyaubert, J.Grisollet, D.Derrier, L.Matringe, M.

    Author Affiliation : Laboratoire de Physiologie Cellulaire Végétale, Institut National de la Recherche Agronomique, UMR 1200, F-38054 Grenoble, France.

    Author Email : michel.matringe@cea.fr

    Journal article : Plant Physiology 2009 Vol.149 No.3 pp.1251-1260

    Abstract : While the presence of a complete shikimate pathway within plant plastidsplastidsSubject Category: Anatomical and Morphological Structures
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    is definitively established, the existence of a cytosolic postchorismate portion of the pathway is still debated. This question is alimented by the presence of a chorismate mutase (CM) within the cytosolcytosolSubject Category: Anatomical and Morphological Structures
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    . Until now, the only known destiny of prephenate, the product of CM, is incorporation into tyrosinetyrosineSubject Category: Chemicals and Chemical Groups
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    (Tyr) and/or phenylalaninephenylalanineSubject Category: Chemicals and Chemical Groups
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    (Phe). Therefore, the presence of a cytosolic CM suggests that enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    involved downstream of CM in Tyr or Phe biosynthesisbiosynthesisSubject Category: Natural Processes
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    could be present within the cytosol of plant cells. It was thus of particular interest to clarify the subcellular localization of arogenate dehydrogenases (TYRAs) and arogenate dehydratases (ADTs), which catalyze the ultimate steps in Tyr and Phe biosynthesis, respectively. The aim of this study was to address this question in Arabidopsis (Arabidopsis thalianaarabidopsis thalianaSubject Category: Organism Names
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    ) by analysis of the subcellular localization of the two TYRAAts and the six AtADTs. This article excludes the occurrence of a spliced TYRAAt1 transcript encoding a cytosolic TYRA protein. Transient expression analyses of TYRA- and ADT-green fluorescent protein fusions reveal that the two Arabidopsis TYRA proteins and the six ADT proteins are all targeted within the plastid. Accordingly, TYRA and ADT proteins were both immunodetected in the chloroplast soluble protein fraction (stroma) of Arabidopsis. No TYRA or ADT proteins were immunodetected in the cytosol of Arabidopsis cells. Taken together, all our data exclude the possibility of Tyr and/or Phe synthesis within the cytosol, at least in green leavesleavesSubject Category: Anatomical and Morphological Structures
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    and Arabidopsis cultured cells.

    ISSN : 0032-0889

    DOI : 10.1104/pp.108.130070

    Record Number : 20103043688

    Publisher : American Society of Plant Biologists

    Location of publication : Rockville

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Arabidopsis thalianaarabidopsis thalianaSubject Category: Organism Names
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    , BrassicalesbrassicalesSubject Category: Organism Names
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    Descriptor(s) : biosynthesisbiosynthesisSubject Category: Natural Processes
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    , catalytic activitycatalytic activitySubject Category: Properties
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    , chloroplastschloroplastsSubject Category: Anatomical and Morphological Structures
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    , cytosolcytosolSubject Category: Anatomical and Morphological Structures
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    , enzyme activityenzyme activitySubject Category: Properties
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , gene expressiongene expressionSubject Category: Miscellaneous
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    , genesgenesSubject Category: Miscellaneous
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    , hydro-lyaseshydro-lyasesSubject Category: Chemicals and Chemical Groups
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    , leavesleavesSubject Category: Anatomical and Morphological Structures
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    , oxidoreductasesoxidoreductasesSubject Category: Chemicals and Chemical Groups
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    , phenylalaninephenylalanineSubject Category: Chemicals and Chemical Groups
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    , plastidsplastidsSubject Category: Anatomical and Morphological Structures
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    , transcriptiontranscriptionSubject Category: Natural Processes
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    , tyrosinetyrosineSubject Category: Chemicals and Chemical Groups
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    Identifier(s) : arogenate dehydratase, arogenate dehydrogenase, Capparales, dehydratases, DNA transcription, redox enzymes

    Broader term(s) : ArabidopsisarabidopsisSubject Category: Organism Names
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    , BrassicaceaebrassicaceaeSubject Category: Organism Names
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    , BrassicalesbrassicalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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