Author(s) :
Hofmann, C.
;
Niehl, A.
;
Sambade, A.
;
Steinmetz, A.
;
Heinlein, M.
Author Affiliation :
Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, 67084 Strasbourg Cedex, France.
Author Email :
manfred.heinlein@ibmp-ulp.u-strasbg.fr
Journal article
:
Plant Physiology
2009
Vol.149
No.4
pp.1810-1823
ref.many
Abstract :
The
tobacco mosaic virustobacco mosaic virusSubject Category: Organism Names
see more details (TMV) movement protein (MP) required for the cell-to-cell spread of viral RNA interacts with the endoplasmic reticulum (ER) as well as with the
cytoskeletoncytoskeletonSubject Category: Anatomical and Morphological Structures
see more details during infection. Whereas associations of MP with ER and microtubules have been intensely investigated, research on the role of
actinactinSubject Category: Chemicals and Chemical Groups
see more details has been rather scarce. We demonstrate that
Nicotiana benthamiananicotiana benthamianaSubject Category: Organism Names
see more details plants transgenic for the actin-binding domain 2 of
Arabidopsis (
Arabidopsis thaliana) fimbrin (AtFIM1) fused to green fluorescent protein (ABD2:GFP) exhibit a dynamic ABD2:GFP-labeled actin cytoskeleton and myosin-dependent Golgi trafficking. These plants also support the movement of TMV. In contrast, both myosin-dependent Golgi trafficking and TMV movement are dominantly inhibited when ABD2:GFP is expressed transiently. Inhibition is mediated through binding of ABD2:GFP to actin filaments, since TMV movement is restored upon disruption of the ABD2:GFP-labeled actin network with latrunculin B. Latrunculin B shows no significant effect on the spread of TMV infection in either wild-type plants or ABD2:GFP
transgenic plantstransgenic plantsSubject Category: Organism Groups
see more details under our treatment conditions. We did not observe any binding of MP along the length of actin filaments. Collectively, these observations demonstrate that TMV movement does not require an intact actomyosin system. Nevertheless, actin-
binding proteinsbinding proteinsSubject Category: Chemicals and Chemical Groups
see more details appear to have the potential to exert control over TMV movement through the inhibition of myosin-associated protein trafficking along the ER membrane.
ISSN :
0032-0889
DOI :
10.1104/pp.108.133827
Record Number :
20103043733
Publisher :
American Society of Plant Biologists
Location of publication :
Rockville
Country of publication :
USA
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
Nicotiana benthamiananicotiana benthamianaSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details, Tobacco mosaic virustobacco mosaic virusSubject Category: Organism Names
see more details, TobamovirustobamovirusSubject Category: Organism Names
see more details, virusesvirusesSubject Category: Organism Names
see more details
Descriptor(s) :
actinactinSubject Category: Chemicals and Chemical Groups
see more details, binding proteinsbinding proteinsSubject Category: Chemicals and Chemical Groups
see more details, cytoskeletoncytoskeletonSubject Category: Anatomical and Morphological Structures
see more details, disease resistancedisease resistanceSubject Category: Properties
see more details, genetically engineered organismsgenetically engineered organismsSubject Category: Organism Groups
see more details, pathogenspathogensSubject Category: Organism Groups
see more details, plant pathogensplant pathogensSubject Category: Organism Groups
see more details, plant virusesplant virusesSubject Category: Organism Groups
see more details, transgenic plantstransgenic plantsSubject Category: Organism Groups
see more details
Identifier(s) :
carrier proteins, genetically engineered plants, genetically modified organisms, genetically modified plants, GEOs, GMOs, phytopathogens, resistance to disease, transgenic organisms, viruses of plants
Broader term(s) :
virusesvirusesSubject Category: Organism Names
see more details, NicotiananicotianaSubject Category: Organism Names
see more details, SolanaceaesolanaceaeSubject Category: Organism Names
see more details, SolanalessolanalesSubject Category: Organism Names
see more details, eudicotseudicotsSubject Category: Organism Names
see more details, angiospermsangiospermsSubject Category: Organism Names
see more details, SpermatophytaspermatophytaSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details, TobamovirustobamovirusSubject Category: Organism Names
see more details, VirgaviridaevirgaviridaeSubject Category: Organism Names
see more details, positive-sense ssRNA Virusespositive-sense ssrna virusesSubject Category: Organism Names
see more details, ssRNA Virusesssrna virusesSubject Category: Organism Names
see more details, RNA Virusesrna virusesSubject Category: Organism Names
see more details