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  • Inhibition of tobacco mosaic virus movement by expression of an actin-binding protein.

    Author(s) : Hofmann, C.Niehl, A.Sambade, A.Steinmetz, A.Heinlein, M.

    Author Affiliation : Institut de Biologie Moléculaire des Plantes du CNRS, Université de Strasbourg, 67084 Strasbourg Cedex, France.

    Author Email : manfred.heinlein@ibmp-ulp.u-strasbg.fr

    Journal article : Plant Physiology 2009 Vol.149 No.4 pp.1810-1823 ref.many

    Abstract : The tobacco mosaic virustobacco mosaic virusSubject Category: Organism Names
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    (TMV) movement protein (MP) required for the cell-to-cell spread of viral RNA interacts with the endoplasmic reticulum (ER) as well as with the cytoskeletoncytoskeletonSubject Category: Anatomical and Morphological Structures
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    during infection. Whereas associations of MP with ER and microtubules have been intensely investigated, research on the role of actinactinSubject Category: Chemicals and Chemical Groups
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    has been rather scarce. We demonstrate that Nicotiana benthamiananicotiana benthamianaSubject Category: Organism Names
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    plants transgenic for the actin-binding domain 2 of Arabidopsis (Arabidopsis thaliana) fimbrin (AtFIM1) fused to green fluorescent protein (ABD2:GFP) exhibit a dynamic ABD2:GFP-labeled actin cytoskeleton and myosin-dependent Golgi trafficking. These plants also support the movement of TMV. In contrast, both myosin-dependent Golgi trafficking and TMV movement are dominantly inhibited when ABD2:GFP is expressed transiently. Inhibition is mediated through binding of ABD2:GFP to actin filaments, since TMV movement is restored upon disruption of the ABD2:GFP-labeled actin network with latrunculin B. Latrunculin B shows no significant effect on the spread of TMV infection in either wild-type plants or ABD2:GFP transgenic plantstransgenic plantsSubject Category: Organism Groups
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    under our treatment conditions. We did not observe any binding of MP along the length of actin filaments. Collectively, these observations demonstrate that TMV movement does not require an intact actomyosin system. Nevertheless, actin-binding proteinsbinding proteinsSubject Category: Chemicals and Chemical Groups
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    appear to have the potential to exert control over TMV movement through the inhibition of myosin-associated protein trafficking along the ER membrane.

    ISSN : 0032-0889

    DOI : 10.1104/pp.108.133827

    Record Number : 20103043733

    Publisher : American Society of Plant Biologists

    Location of publication : Rockville

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Nicotiana benthamiananicotiana benthamianaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , Tobacco mosaic virustobacco mosaic virusSubject Category: Organism Names
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    , TobamovirustobamovirusSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    Descriptor(s) : actinactinSubject Category: Chemicals and Chemical Groups
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    , binding proteinsbinding proteinsSubject Category: Chemicals and Chemical Groups
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    , cytoskeletoncytoskeletonSubject Category: Anatomical and Morphological Structures
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    , disease resistancedisease resistanceSubject Category: Properties
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    , genetically engineered organismsgenetically engineered organismsSubject Category: Organism Groups
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    , pathogenspathogensSubject Category: Organism Groups
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    , plant pathogensplant pathogensSubject Category: Organism Groups
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    , plant virusesplant virusesSubject Category: Organism Groups
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    , transgenic plantstransgenic plantsSubject Category: Organism Groups
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    Identifier(s) : carrier proteins, genetically engineered plants, genetically modified organisms, genetically modified plants, GEOs, GMOs, phytopathogens, resistance to disease, transgenic organisms, viruses of plants

    Broader term(s) : virusesvirusesSubject Category: Organism Names
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    , NicotiananicotianaSubject Category: Organism Names
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    , SolanaceaesolanaceaeSubject Category: Organism Names
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    , SolanalessolanalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , TobamovirustobamovirusSubject Category: Organism Names
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    , VirgaviridaevirgaviridaeSubject Category: Organism Names
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    , positive-sense ssRNA Virusespositive-sense ssrna virusesSubject Category: Organism Names
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    , ssRNA Virusesssrna virusesSubject Category: Organism Names
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    , RNA Virusesrna virusesSubject Category: Organism Names
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