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  • The Arabidopsis callose synthase gene GSL8 is required for cytokinesis and cell patterning.

    Author(s) : Chen XiongYanLiu LinLee EunKyoungHan XiaoRim YeongGilChu HyoSubKim SeonWonSack, F.Kim JaeYean

    Author Affiliation : Division of Applied Life Science (BK21 Program), Graduate School of Gyeongsang National University, Plant Molecular Biology and Biotechnology Research Center, Environmental Biotechnology National Core Research Center, Jinju 660-701, Korea Republic.

    Author Email : kimjy@gnu.ac.kr

    Journal article : Plant Physiology 2009 Vol.150 No.1 pp.105-113

    Abstract : Cytokinesis is the division of the cytoplasm and its separation into two daughter cells. Cell plate growth and cytokinesis appear to require callosecalloseSubject Category: Chemicals and Chemical Groups
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    , but direct functional evidence is still lacking. To determine the role of callose and its synthesis during cytokinesis, we identified and characterized mutantsmutantsSubject Category: Miscellaneous
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    in many members of the GLUCAN SYNTHASE-LIKE (GSL; or CALLOSE SYNTHASE) gene family in Arabidopsis (Arabidopsis thalianaarabidopsis thalianaSubject Category: Organism Names
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    ). Most gsl mutants (gsl1-gsl7, gsl9, gsl11, and gsl12) exhibited roughly normal seedling growthseedling growthSubject Category: Miscellaneous
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    and development. However, mutationsmutationsSubject Category: Miscellaneous
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    in GSL8, which were previously reported to be gametophytic lethal, were found to produce seedlingsseedlingsSubject Category: Miscellaneous
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    with pleiotropic defects during embryogenesis and early vegetative growth. We found cell wall stubs, two nuclei in one cell, and other defects in cell divisioncell divisionSubject Category: Natural Processes
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    in homozygous gsl8 insertional allelesallelesSubject Category: Miscellaneous
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    . In addition, gsl8 mutants and inducible RNA interferencerna interferenceSubject Category: Miscellaneous
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    lines of GSL8 showed reduced callose deposition at cell plates and/or new cell walls. Together, these data show that the GSL8 gene encodes a putative callose synthase required for cytokinesis and seedling maturation. In addition, gsl8 mutants disrupt cellular and tissue-level patterning, as shown by the presence of clusters of stomata in direct contact and by islands of excessive cell proliferation in the developing epidermis. Thus, GSL8 is required for patterning as well as cytokinesis during Arabidopsis development.

    ISSN : 0032-0889

    DOI : 10.1104/pp.108.133918

    Record Number : 20103043759

    Publisher : American Society of Plant Biologists

    Location of publication : Rockville

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Arabidopsis thalianaarabidopsis thalianaSubject Category: Organism Names
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    , BrassicalesbrassicalesSubject Category: Organism Names
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    Descriptor(s) : allelesallelesSubject Category: Miscellaneous
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    , biosynthesisbiosynthesisSubject Category: Natural Processes
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    , callosecalloseSubject Category: Chemicals and Chemical Groups
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    , cell divisioncell divisionSubject Category: Natural Processes
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    , cell growthcell growthSubject Category: Natural Processes
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , gene expressiongene expressionSubject Category: Miscellaneous
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    , genesgenesSubject Category: Miscellaneous
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    , mutantsmutantsSubject Category: Miscellaneous
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    , mutationsmutationsSubject Category: Miscellaneous
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    , plant developmentplant developmentSubject Category: Natural Processes
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    , RNA interferencerna interferenceSubject Category: Miscellaneous
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    , seedling growthseedling growthSubject Category: Miscellaneous
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    , seedlingsseedlingsSubject Category: Miscellaneous
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    Identifier(s) : callose synthase, Capparales, cell elongation, karyokinesis, RNAi

    Broader term(s) : ArabidopsisarabidopsisSubject Category: Organism Names
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    , BrassicaceaebrassicaceaeSubject Category: Organism Names
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    , BrassicalesbrassicalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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