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  • Arabidopsis separase functions beyond the removal of sister chromatid cohesion during meiosis.

    Author(s) : Yang, X. H.Boateng, K. A.Strittmatter, L.Burgess, R.Makaroff, C. A.

    Author Affiliation : Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA.

    Author Email : makaroca@muohio.edu

    Journal article : Plant Physiology 2009 Vol.151 No.1 pp.323-333 ref.many

    Abstract : Separase is a capase family protease that is required for the release of sister chromatid cohesion during meiosismeiosisSubject Category: Natural Processes
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    and mitosismitosisSubject Category: Natural Processes
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    . Proteolytic cleavage of the -kleisin subunit of the cohesin complex at the metaphasemetaphaseSubject Category: Miscellaneous
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    -to-anaphaseanaphaseSubject Category: Miscellaneous
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    transition is essential for the proper segregation of chromosomeschromosomesSubject Category: Anatomical and Morphological Structures
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    . In addition to its highly conserved role in cleaving the -kleisin subunit, separase appears to have acquired additional diverse activities in some organisms, including involvement in mitotic and meiotic anaphase spindle assembly and elongation, interphase spindle pole body positioning, and epithelial cell reorganization. Results from the characterization of Arabidopsis (Arabidopsis thalianaarabidopsis thalianaSubject Category: Organism Names
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    ) separase (ESP) demonstrated that meiotic expression of ESP RNA interferencerna interferenceSubject Category: Miscellaneous
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    blocked the proper removal of cohesin from chromosomes and resulted in the presence of a mixture of fragmented chromosomes and intact bivalents. The presence of large numbers of intact bivalents raised the possibility that separase may also have multiple roles in Arabidopsis. In this report, we show that meiotic expression of ESP RNA interference blocks the removal of cohesin during both meiosis I and II, results in alterations in nonhomologous centromere association, disrupts the radial microtubule system after telophase II, and affects the proper establishment of nuclear cytoplasmic domains, resulting in the formation of multinucleate microspores.

    ISSN : 0032-0889

    DOI : 10.1104/pp.109.140699

    Record Number : 20103043917

    Publisher : American Society of Plant Biologists

    Location of publication : Rockville

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Arabidopsis thalianaarabidopsis thalianaSubject Category: Organism Names
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    , BrassicalesbrassicalesSubject Category: Organism Names
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    Descriptor(s) : anaphaseanaphaseSubject Category: Miscellaneous
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    , centromerescentromeresSubject Category: Anatomical and Morphological Structures
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    , chromatidschromatidsSubject Category: Anatomical and Morphological Structures
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    , chromosomeschromosomesSubject Category: Anatomical and Morphological Structures
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , meiosismeiosisSubject Category: Natural Processes
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    , metaphasemetaphaseSubject Category: Miscellaneous
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    , mitosismitosisSubject Category: Natural Processes
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    , proteinasesproteinasesSubject Category: Chemicals and Chemical Groups
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    , RNA interferencerna interferenceSubject Category: Miscellaneous
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    Identifier(s) : capase, Capparales, proteases, reduction division, RNAi, separase

    Broader term(s) : ArabidopsisarabidopsisSubject Category: Organism Names
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    , BrassicaceaebrassicaceaeSubject Category: Organism Names
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    , BrassicalesbrassicalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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