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  • Null mutation of the MdACS3 gene, coding for a ripening-specific 1-aminocyclopropane-1-carboxylate synthase, leads to long shelf life in apple fruit.

    Author(s) : Wang AideYamakake, J.Kudo, H.Wakasa, Y.Hatsuyama, Y.Igarashi, M.Kasai, A.Li TianZhongHarada, T.

    Author Affiliation : Laboratory of Plant Breeding and Genetics, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561, Japan.

    Author Email : tharada@cc.hirosaki-u.ac.jp

    Journal article : Plant Physiology 2009 Vol.151 No.1 pp.391-399 ref.34

    Abstract : Expression of MdACS1, coding for 1-aminocyclopropane-1-carboxylate synthase1-aminocyclopropane-1-carboxylate synthaseSubject Category: Chemicals and Chemical Groups
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    (ACS), parallels the level of ethyleneethyleneSubject Category: Chemicals and Chemical Groups
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    production in ripeningripeningSubject Category: Natural Processes
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    apple (Malus domesticamalus domesticaSubject Category: Organism Names
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    ) fruit. Here we show that expression of another ripening-specific ACS gene (MdACS3) precedes the initiation of MdACS1 expression by approximately 3 weeks; MdACS3 expression then gradually decreases as MdACS1 expression increases. Because MdACS3 expression continues in ripening fruit treated with 1-methylcyclopropene, its transcription appears to be regulated by a negative feedback mechanism. Three genesgenesSubject Category: Miscellaneous
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    in the MdACS3 family (a, b, and c) were isolated from a genomic library, but two of them (MdACS3b and MdACS3c) possess a 333-bp transposon-like insertion in their 5′ flanking region that may prevent transcription of these genes during ripening. A single nucleotide polymorphism in the coding region of MdACS3a results in an amino acid substitution (glycine-289 → valine) in the active site that inactivates the enzyme. Furthermore, another null allele of MdACS3a, Mdacs3a, showing no ability to be transcribed, was found by DNA sequencingdna sequencingSubject Category: Techniques, Methodologies and Equipment
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    . Apple cultivarscultivarsSubject Category: Organism Groups
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    homozygous or heterozygous for both null allelotypes showed no or very low expression of ripening-related genes and maintained fruit firmnessfirmnessSubject Category: Properties
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    . These results suggest that MdACS3a plays a crucial role in regulation of fruit ripening in apple, and is a possible determinant of ethylene production and shelf life in apple fruit.

    ISSN : 0032-0889

    DOI : 10.1104/pp.109.135822

    Record Number : 20103043920

    Publisher : American Society of Plant Biologists

    Location of publication : Rockville

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : MalusmalusSubject Category: Organism Names
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    , Malus domesticamalus domesticaSubject Category: Organism Names
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    Descriptor(s) : 1-aminocyclopropane-1-carboxylate synthase1-aminocyclopropane-1-carboxylate synthaseSubject Category: Chemicals and Chemical Groups
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    , allelesallelesSubject Category: Miscellaneous
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    , amino acidsamino acidsSubject Category: Chemicals and Chemical Groups
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    , applesapplesSubject Category: Commodities and Products
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    , cultivarscultivarsSubject Category: Organism Groups
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    , DNAdnaSubject Category: Chemicals and Chemical Groups
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    , DNA sequencingdna sequencingSubject Category: Techniques, Methodologies and Equipment
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    , ethyleneethyleneSubject Category: Chemicals and Chemical Groups
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    , firmnessfirmnessSubject Category: Properties
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    , fruitsfruitsSubject Category: Anatomical and Morphological Structures
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    , gene expressiongene expressionSubject Category: Miscellaneous
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    , genesgenesSubject Category: Miscellaneous
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    , genomesgenomesSubject Category: Miscellaneous
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    , genomicsgenomicsSubject Category: Disciplines, Occupations and Industries
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    , mutantsmutantsSubject Category: Miscellaneous
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    , mutationsmutationsSubject Category: Miscellaneous
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    , nucleotidesnucleotidesSubject Category: Chemicals and Chemical Groups
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    , plant growth regulatorsplant growth regulatorsSubject Category: Chemicals and Chemical Groups
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    , ripeningripeningSubject Category: Natural Processes
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    , storage lifestorage lifeSubject Category: Time Periods
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    , transposable elementstransposable elementsSubject Category: Miscellaneous
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    Identifier(s) : ACC synthase, cultivated varieties, deoxyribonucleic acid, DNA insertion elements, insertion elements, insertion sequences, mobile genetic elements, mobile sequences, nucleotide sequence analysis, nucleotide sequencing, plant growth substances, plant hormones, transposons

    Broader term(s) : RosaceaerosaceaeSubject Category: Organism Names
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    , RosalesrosalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , MalusmalusSubject Category: Organism Names
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