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  • Plant Elongator regulates auxin-related genes during RNA polymerase II transcription elongation.

    Author(s) : Nelissen, H.Groeve, S. deFleury, D.Neyt, P.Bruno, L.Bitonti, M. B.Vandenbussche, F.Straeten, D. van derYamaguchi, T.Tsukaya, H.Witters, E.Jaeger, G. deHouben, A.Lijsebettens, M. van

    Author Affiliation : Department of Plant Systems Biology, Flanders Institute for Biotechnology, Ghent University, Technologiepark 927, 9052 Ghent, Belgium.

    Author Email : mieke.vanlijsebettens@psb.vib-ugent.be

    Journal article : Proceedings of the National Academy of Sciences of the United States of America 2010 Vol.107 No.4 pp.1678-1683 ref.36

    Abstract : In eukaryotes, transcription of protein-encoding genes is strongly regulated by posttranslational modifications of histones that affect the accessibility of the DNA by RNA polymerase II (RNAPII). The Elongator complex was originally identified in yeast as a histone acetyltransferase (HAT) complex that activates RNAPII-mediated transcription. In Arabidopsis thaliana, the Elongator mutants elo1, elo2, and elo3 with decreased leaf and primary root growth due to reduced cell proliferation identified homologs of components of the yeast Elongator complex, Elp4, EIp1, and EIp3, respectively. Here we show that the Elongator complex was purified from plant cell cultures as a six-component complex. The role of plant Elongator in transcription elongation was supported by colocalization of the HAT enzyme, ELO3, with euchromatin and the phosphorylated form of RNAPII, and reduced histone H3 lysine 14 acetylation at the coding region of the SHORT HYPOCOTYL 2 auxin repressor and the LAX2 auxin influx carrier gene with reduced expression levels in the elo3 mutant. Additional auxin-related genes were down-regulated in the transcriptome of elo mutants but not targeted by the Elongator HAT activity showing specificity in target gene selection. Biological relevance was apparent by auxin-related phenotypes and marker gene analysis. Ethylene and jasmonic acid signaling and abiotic stress responses were up-regulated in the elo transcriptome and might contribute to the pleiotropic elo phenotype. Thus, although the structure of Elongator and its substrate are conserved, target gene selection has diverged, showing that auxin signaling and influx are under chromatin control.

    ISSN : 0027-8424

    DOI : 10.1073/pnas.0913559107

    URL : http://www.pnas.org/

    Record Number : 20103059409

    Publisher : National Academy of Sciences

    Location of publication : Washington

    Country of publication : USA

    Language of text : English

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Arabidopsis thaliana, Brassicales

    Descriptor(s) : acetylation, auxins, cell division, chromatin, DNA-directed RNA polymerase, endogenous growth regulators, enzymes, ethylene, gene expression, genes, growth, histones, jasmonic acid, leaves, marker genes, mutants, mutations, phenotypes, plant growth regulators, roots, signal transduction, stress, stress response, transcription, transcriptomes

    Identifier(s) : Capparales, DNA transcription, histone acetyltransferase, karyokinesis, plant growth substances, plant hormones, RNA nucleotidyltransferase, RNA polymerase

    Broader term(s) : Arabidopsis, Brassicaceae, Brassicales, eudicots, angiosperms, Spermatophyta, plants, eukaryotes