Author(s) :
Vujčić, Z.
;
Lončar, N.
;
Dojnov, B.
;
Milovanović, A.
;
Vujčić, M.
;
Božić, N.
Author Affiliation :
Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
Author Email :
zvujcic@chem.bg.ac.rs
Journal article
:
Food Chemistry
2010
Vol.121
No.2
pp.418-423
ref.30
Abstract :
Potato juice (a waste product from the starch industry) is a potential source of novel
enzymesenzymesSubject Category: Chemicals and Chemical Groups
see more details for food applications. For use in the production and improvement of food protein hydrolysates, commercially available exopeptidases, predominantly aminopeptidases, are recommended. The present study was performed to explore possible biotechnological interest of
leucyl aminopeptidaseleucyl aminopeptidaseSubject Category: Chemicals and Chemical Groups
see more details (LAP) activity in the potato tuber. The LAP from potato tuber was purified and characterised. Specific LAP activity was increased 200-fold by purification of the crude extract. The purified enzyme had a pH optimum of 9.0 and temperature optimum of 45°C. LAP hydrolysed leucine-, alanine- and lysine-
p-nitroanilide to a similar degree. The most efficient inhibitor was 1,10-phenanthroline. Almost all divalent
cationscationsSubject Category: Chemicals and Chemical Groups
see more details tested inhibited the
enzyme activityenzyme activitySubject Category: Properties
see more details, while Co
2+ stimulated LAP activity by over 100%. The purified LAP had a
molecular weightmolecular weightSubject Category: Properties
see more details of 90 kDa with an
isoelectric pointisoelectric pointSubject Category: Properties
see more details of 5.45. Sodium dodecylsulfate-polyacrylamide gel electrophoresis revealed one band of 48 kDa.
ISSN :
0308-8146
DOI :
10.1016/j.foodchem.2009.12.058
Record Number :
20103101356
Publisher :
Elsevier Ltd
Location of publication :
Oxford
Country of publication :
UK
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
Solanum tuberosumsolanum tuberosumSubject Category: Organism Names
see more details
Descriptor(s) :
cationscationsSubject Category: Chemicals and Chemical Groups
see more details, cobaltcobaltSubject Category: Chemicals and Chemical Groups
see more details, enzyme activityenzyme activitySubject Category: Properties
see more details, enzyme inhibitorsenzyme inhibitorsSubject Category: Chemicals and Chemical Groups
see more details, enzymesenzymesSubject Category: Chemicals and Chemical Groups
see more details, hydrolysishydrolysisSubject Category: Miscellaneous
see more details, isoelectric pointisoelectric pointSubject Category: Properties
see more details, leucyl aminopeptidaseleucyl aminopeptidaseSubject Category: Chemicals and Chemical Groups
see more details, molecular weightmolecular weightSubject Category: Properties
see more details, potatoespotatoesSubject Category: Commodities and Products
see more details, tuberstubersSubject Category: Anatomical and Morphological Structures
see more details
Identifier(s) :
cytosol aminopeptidase, leucine aminopeptidase
Broader term(s) :
SolanumsolanumSubject Category: Organism Names
see more details, SolanaceaesolanaceaeSubject Category: Organism Names
see more details, SolanalessolanalesSubject Category: Organism Names
see more details, eudicotseudicotsSubject Category: Organism Names
see more details, angiospermsangiospermsSubject Category: Organism Names
see more details, SpermatophytaspermatophytaSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details