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  • Characterisation of leucyl aminopeptidase from Solanum tuberosum tuber.

    Author(s) : Vujčić, Z.Lončar, N.Dojnov, B.Milovanović, A.Vujčić, M.Božić, N.

    Author Affiliation : Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.

    Author Email : zvujcic@chem.bg.ac.rs

    Journal article : Food Chemistry 2010 Vol.121 No.2 pp.418-423 ref.30

    Abstract : Potato juice (a waste product from the starch industry) is a potential source of novel enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    for food applications. For use in the production and improvement of food protein hydrolysates, commercially available exopeptidases, predominantly aminopeptidases, are recommended. The present study was performed to explore possible biotechnological interest of leucyl aminopeptidaseleucyl aminopeptidaseSubject Category: Chemicals and Chemical Groups
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    (LAP) activity in the potato tuber. The LAP from potato tuber was purified and characterised. Specific LAP activity was increased 200-fold by purification of the crude extract. The purified enzyme had a pH optimum of 9.0 and temperature optimum of 45°C. LAP hydrolysed leucine-, alanine- and lysine-p-nitroanilide to a similar degree. The most efficient inhibitor was 1,10-phenanthroline. Almost all divalent cationscationsSubject Category: Chemicals and Chemical Groups
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    tested inhibited the enzyme activityenzyme activitySubject Category: Properties
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    , while Co2+ stimulated LAP activity by over 100%. The purified LAP had a molecular weightmolecular weightSubject Category: Properties
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    of 90 kDa with an isoelectric pointisoelectric pointSubject Category: Properties
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    of 5.45. Sodium dodecylsulfate-polyacrylamide gel electrophoresis revealed one band of 48 kDa.

    ISSN : 0308-8146

    DOI : 10.1016/j.foodchem.2009.12.058

    Record Number : 20103101356

    Publisher : Elsevier Ltd

    Location of publication : Oxford

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Solanum tuberosumsolanum tuberosumSubject Category: Organism Names
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    Descriptor(s) : cationscationsSubject Category: Chemicals and Chemical Groups
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    , cobaltcobaltSubject Category: Chemicals and Chemical Groups
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    , enzyme activityenzyme activitySubject Category: Properties
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    , enzyme inhibitorsenzyme inhibitorsSubject Category: Chemicals and Chemical Groups
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , hydrolysishydrolysisSubject Category: Miscellaneous
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    , isoelectric pointisoelectric pointSubject Category: Properties
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    , leucyl aminopeptidaseleucyl aminopeptidaseSubject Category: Chemicals and Chemical Groups
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    , molecular weightmolecular weightSubject Category: Properties
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    , potatoespotatoesSubject Category: Commodities and Products
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    , tuberstubersSubject Category: Anatomical and Morphological Structures
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    Identifier(s) : cytosol aminopeptidase, leucine aminopeptidase

    Broader term(s) : SolanumsolanumSubject Category: Organism Names
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    , SolanaceaesolanaceaeSubject Category: Organism Names
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    , SolanalessolanalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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