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  • Oxidative stress and metal ions regulate a ferritin-like gene, dpr, in Streptococcus pyogenes.

    Author(s) : Tsou ChihChengChuan ChiangNiLin YeeShinChuang WoeiJerLin, M. T.Liu ChingChuanWu JiunnJong

    Author Affiliation : Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, No. 1, University Road, Tainan, Taiwan.

    Author Email : jjwu@mail.ncku.edu.tw

    Journal article : International Journal of Medical Microbiology 2010 Vol.300 No.4 pp.259-264 ref.41

    Abstract : Bacteria encounter oxidative stress by exposure to reactive oxygen species (ROS) present in the aerobic environment and during immune responses. In Streptococcus pyogenes, Dpr has been identified as a stress protein conferring resistance to hydrogen peroxide and multiple other stresses. The expression of Dpr is under perR (peroxide stress response regulator) control. However, the exact molecular mechanism of PerR regulation of Dpr is not clear. In this study, a perR deletion mutant was constructed by double cross-over mutagenesis. The profile of Dpr expression, performed by Western blot assay, revealed growth-phase dependency under normal culture conditions. Dpr expression decreased under iron-restricted conditions, whereas iron, zinc, nickel, and hydrogen peroxide induced its expression. The perR mutant does not induce Dpr as well when exposed to environmental signals. PerR binds the promoter region of dpr. Increased iron and hydrogen peroxide concentrations decreased PerR binding to the promoter region of dpr, suggesting that regulation of Dpr by environmental signals is mediated by PerR directly.

    ISSN : 1438-4221

    DOI : 10.1016/j.ijmm.2009.09.002

    URL : http://www.sciencedirect.com/science/...

    Record Number : 20103178548

    Publisher : Elsevier GmbH

    Location of publication : München

    Country of publication : Germany

    Language of text : English

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Streptococcus pyogenes

    Descriptor(s) : gene expression, genes, genetic regulation, hydrogen peroxide, iron, iron binding capacity, metal ions, mutants, nickel, oxidative stress, promoters, zinc

    Identifier(s) : bacterium, peroxide stress response regulator, promoter region, promoter sequences

    Broader term(s) : Streptococcus, Streptococcaceae, Lactobacillales, Bacilli, Firmicutes, Bacteria, prokaryotes