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  • The production of fluorescent transgenic trout to study in vitro myogenic cell differentiation.

    Author(s) : Gabillard, J. C.Ralliere, C.Sabin, N.Rescan, P. Y.

    Author Affiliation : National Institute for Agricultural Research, Joint Research Unit for Fish Physiology, Biodiversity and the Environment, INRA Scribe, IFR140, Campus de Beaulieu, 35042 Rennes, France.

    Author Email : jean-charles.gabillard@rennes.inra.frcecile.ralliere@rennes.Iinra.frnathalie.sabin@rennes.inra.frpierre-yves.rescan@rennes.inra.fr

    Journal article : BMC Biotechnology 2010 Vol.10 No.39 pp.(17 May 2010) ref.29

    Abstract : Background: Fish skeletal muscleskeletal muscleSubject Category: Anatomical and Morphological Structures
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    growth involves the activation of a resident myogenic stem cell population, referred to as satellite cells, that can fuse with pre-existing muscle fibersmuscle fibersSubject Category: Anatomical and Morphological Structures
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    muscle fibersSubject Category: Anatomical and Morphological Structures
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    or among themselves to generate a new fiber. In order to monitor the regulation of myogenic cell differentiationcell differentiationSubject Category: Natural Processes
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    and fusion by various extrinsic factors, we generated transgenic trout (OncorhynchusoncorhynchusSubject Category: Organism Names
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    mykiss
    ) carrying a construct containing the green fluorescent proteingreen fluorescent proteinSubject Category: Chemicals and Chemical Groups
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    reporter gene driven by a fast myosin light chain (MylC2) promoter, and cultivated genetically modified myogenic cells derived from these fish. Results: In transgenic trout, green fluorescence appeared in fast muscle fibers as early as the somitogenesis stage and persisted throughout life. Using an in vitro myogenesis system we observed that satellite cells isolated from the myotomal muscle of transgenic trout expressed GFP about 5 days post-plating as they started to fuse. GFP fluorescence persisted subsequently in myosatellite cell-derived myotubes. Using this in vitro myogenesis system, we showed that the rate of muscle cell differentiation was strongly dependent on temperature, one of the most important environmental factorsenvironmental factorsSubject Category: Properties
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    in the muscle growth of poikilotherms. Conclusion: We produced MyLC2-gfp transgenic trout that exhibited fluorescence in their fast muscle fibers. The culture of muscle cells extracted from these trout enabled the real-time monitoring of myogenic differentiation. This in vitro myogenesis system could have numerous applications in fish physiology to evaluate the myogenic activity of secreted growth factorsgrowth factorsSubject Category: Chemicals and Chemical Groups
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    , to test interfering RNA and to assess the myogenic potential of fish mesenchymal stem cellsstem cellsSubject Category: Anatomical and Morphological Structures
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    . In ecotoxicology, this system could be useful to asses the impact of environmental factors and marine pollutantspollutantsSubject Category: Miscellaneous
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    on fish muscle growth.

    ISSN : 1472-6750

    URL : http://www.biomedcentral.com/content/...

    Record Number : 20103180373

    Publisher : BioMed Central Ltd

    Location of publication : London

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : OncorhynchusoncorhynchusSubject Category: Organism Names
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    , rainbow troutrainbow troutSubject Category: Organism Names
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    , trouttroutSubject Category: Organism Names
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    Descriptor(s) : animal physiologyanimal physiologySubject Category: Miscellaneous
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    , aquatic animalsaquatic animalsSubject Category: Organism Groups
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    , aquatic organismsaquatic organismsSubject Category: Organism Groups
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    , cell culturecell cultureSubject Category: Techniques, Methodologies and Equipment
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    , cell differentiationcell differentiationSubject Category: Natural Processes
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    , environmental factorsenvironmental factorsSubject Category: Properties
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    , genesgenesSubject Category: Miscellaneous
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    , genetically engineered organismsgenetically engineered organismsSubject Category: Organism Groups
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    , green fluorescent proteingreen fluorescent proteinSubject Category: Chemicals and Chemical Groups
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    , growth factorsgrowth factorsSubject Category: Chemicals and Chemical Groups
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    , muscle fibresmuscle fibresSubject Category: Anatomical and Morphological Structures
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    , myosinsmyosinsSubject Category: Chemicals and Chemical Groups
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    , pollutantspollutantsSubject Category: Miscellaneous
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    , promoterspromotersSubject Category: Chemicals and Chemical Groups
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    , reporter genesreporter genesSubject Category: Miscellaneous
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    , RNArnaSubject Category: Chemicals and Chemical Groups
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    , RNA interferencerna interferenceSubject Category: Miscellaneous
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    , skeletal muscleskeletal muscleSubject Category: Anatomical and Morphological Structures
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    , stem cellsstem cellsSubject Category: Anatomical and Morphological Structures
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    , toxicologytoxicologySubject Category: Disciplines, Occupations and Industries
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    , transgenic animalstransgenic animalsSubject Category: Organism Groups
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    , water pollutionwater pollutionSubject Category: Miscellaneous
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    Identifier(s) : aquatic species, cytodifferentiation, genetically engineered animals, genetically modified animals, genetically modified organisms, GEOs, GMOs, muscle fibersmuscle fibersSubject Category: Anatomical and Morphological Structures
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    , myosin, Oncorhynchus mykiss, promoter region, promoter sequences, reporter gene, ribonucleic acid, RNAi, transgenic organisms

    Broader term(s) : OncorhynchusoncorhynchusSubject Category: Organism Names
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    , SalmonidaesalmonidaeSubject Category: Organism Names
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    , SalmoniformessalmoniformesSubject Category: Organism Names
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    , OsteichthyesosteichthyesSubject Category: Organism Names
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    , fishesfishesSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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