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  • Effects of lacquer polysaccharides, glycoproteins and isoenzymes on the activity of free and immobilised laccase from Rhus vernicifera.

    Author(s) : Wan YunYangLu RongAkiyama, K.Okamoto, K.Honda, T.Du YuMinYoshida, T.Miyakoshi, T.Knill, C. J.Kennedy, J. F.

    Author Affiliation : College of Resource and Environmental Science, Wuhan University, Wuhan 430072, Hubei, China.

    Author Email : jfk@chembiotech.co.uk

    Journal article : International Journal of Biological Macromolecules 2010 Vol.47 No.1 pp.76-81

    Abstract : The purified polysaccharidespolysaccharidesSubject Category: Chemicals and Chemical Groups
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    , glycoproteinsglycoproteinsSubject Category: Chemicals and Chemical Groups
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    , and isoenzymesisoenzymesSubject Category: Chemicals and Chemical Groups
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    of RhusrhusSubject Category: Organism Names
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    laccaselaccaseSubject Category: Chemicals and Chemical Groups
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    , and crude enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , from Chinese lacquer (Rhus vernicifera sapsapSubject Category: Miscellaneous
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    ) were used to determine their influence on the enzymic activity of Rhus laccase on several substrates (4-phenylenediamine, isoeugenol and coniferyl alcohol). No product identity changes were observed when these components were added singularly or in combination to the enzymic reactions (only relative product yields varied significantly), however, the polysaccharides (GP1 and GP2) and glycoprotein (stellacyanin, St) exhibited negative effects, and the two isoenzymes (L1 and L2) exhibited positive synergistic effects, on the activity of Rhus laccase. With respect to the activity of the crude enzymes, the negative effects of GP1, GP2 and St were greater than the positive effects of L1 and L2, compared with free Rhus laccase on its own (using 4-phenylenediamine as substrate), the estimated inhibitory effect (of GP1, GP2 and St) being by at least a factor of 50 (even with the positive effect of L1 and L2). This contributes to understanding of lacquer storage stability and drying rates. Immobilisation of crude enzymes using a variety of techniques (using natural and modified polysaccharides, and an inorganic support) where evaluated using isoeugenol as substrate. Agar embedding and zirconium chloride chelation methods resulted in the highest substrate conversion levels. The yields and products of isoeugenol catalysis using Vietnamese crude enzymes/purified Rhus laccase and commercial Denilite laccase were also compared and contrasted with their Chinese lacquer sap equivalents.

    ISSN : 0141-8130

    DOI : 10.1016/j.ijbiomac.2010.03.016

    Record Number : 20103180411

    Publisher : Elsevier Ltd

    Location of publication : Oxford

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : RhusrhusSubject Category: Organism Names
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    Descriptor(s) : enzyme activityenzyme activitySubject Category: Properties
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , glycoproteinsglycoproteinsSubject Category: Chemicals and Chemical Groups
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    , isoenzymesisoenzymesSubject Category: Chemicals and Chemical Groups
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    , laccaselaccaseSubject Category: Chemicals and Chemical Groups
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    , polysaccharidespolysaccharidesSubject Category: Chemicals and Chemical Groups
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    , sapsapSubject Category: Miscellaneous
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    , varnishesvarnishesSubject Category: Commodities and Products
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    Identifier(s) : complex carbohydrates, isozymes, lacquers, Rhus vernicifera

    Broader term(s) : AnacardiaceaeanacardiaceaeSubject Category: Organism Names
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    , SapindalessapindalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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