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  • Molecular basis of neurovirulence of flury rabies virus vaccine strains: importance of the polymerase and the glycoprotein R333Q mutation.

    Author(s) : Tao LiHongGe JinYingWang XiJunZhai HongYueHua TaoZhao BoLinKong DongNiYang, C. L.Chen HuaLanBu ZhiGao

    Author Affiliation : Veterinary Public Health Laboratory of Ministry of Agriculture, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, China.

    Author Email : zgbu@yahoo.comhlchen1@yahoo.com

    Journal article : Journal of Virology 2010 Vol.84 No.17 pp.8926-8936 ref.8

    Abstract : The molecular mechanisms associated with rabies virusrabies virusSubject Category: Organism Names
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    (RV) virulencevirulenceSubject Category: Miscellaneous
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    are not fully understood. In this study, the RV Flury low-egg-passage (LEP) and high-egg-passage (HEP) strains were used as models to explore the attenuation mechanism of RV. The results of our studies confirmed that the R333Q mutation in the glycoprotein (GR333Q) is crucial for the attenuation of Flury RV in micemiceSubject Category: Organism Names
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    . The R333Q mutation is stably maintained in the HEP genome background but not in the LEP genome background during replication in mouse brain tissue or cell culture. Further investigation using chimeric viruses revealed that the polymerase L gene determines the genetic stability of the GR333Q mutation during replication. Moreover, a recombinant RV containing the LEP G protein with the R333Q mutation and the HEP L gene showed significant attenuation, genetic stability, enhancement of apoptosis, and immunogenicity. These results indicate that attenuation of the RV Flury strain results from the coevolution of G and L elements and provide important information for the generation of safer and more effective modified live rabies vaccine.

    ISSN : 0022-538X

    DOI : 10.1128/JVI.00787-10

    Record Number : 20103261632

    Publisher : American Society for Microbiology (ASM)

    Location of publication : Washington

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : micemiceSubject Category: Organism Names
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    , Rabies virusrabies virusSubject Category: Organism Names
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    Descriptor(s) : animal modelsanimal modelsSubject Category: Techniques, Methodologies and Equipment
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    , genesgenesSubject Category: Miscellaneous
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    , genomesgenomesSubject Category: Miscellaneous
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    , glycoproteinsglycoproteinsSubject Category: Chemicals and Chemical Groups
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    , mutationsmutationsSubject Category: Miscellaneous
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    , viral proteinsviral proteinsSubject Category: Chemicals and Chemical Groups
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    , viral replicationviral replicationSubject Category: Miscellaneous
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    , virulencevirulenceSubject Category: Miscellaneous
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    Broader term(s) : MuridaemuridaeSubject Category: Organism Names
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    , rodentsrodentsSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , LyssaviruslyssavirusSubject Category: Organism Names
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    , RhabdoviridaerhabdoviridaeSubject Category: Organism Names
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    , MononegaviralesmononegaviralesSubject Category: Organism Names
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    , negative-sense ssRNA Virusesnegative-sense ssrna virusesSubject Category: Organism Names
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    , ssRNA Virusesssrna virusesSubject Category: Organism Names
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    , RNA Virusesrna virusesSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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