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  • Potential antiparasitic activity of pomegranate extracts against schistosomules and mature worms of Schistosoma mansoni: in vitro and in vivo study.

    Author(s) : Fahmy, Z. H.El-Shennawy, A. M.El-Komy, W.Ali, E.Hamid, S. S. A.

    Author Affiliation : Department of Parasitology, Theodore Bilharz Research Institute, Imbaba P.O.Box.30, Giza, Egypt.

    Journal article : Australian Journal of Basic and Applied Sciences 2009 Vol.3 No.4 pp.4634-4643 ref.33

    Abstract : The use of medicinal plantsmedicinal plantsSubject Category: Organism Groups
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    medicinal plantsSubject Category: Commodities and Products
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    has gained much attention in the last decade, and among those plants commonly used as medication in folk medicine, various extracts have been the subject of many pharmacological studies. Pomegranate (Punica granatumpunica granatumSubject Category: Organism Names
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    ) a fruit with rich ethnomedical applications, has been also introduced as potential anti-microbial and anti-helminthic agent. This study was designed to estimate its in vivo and in vitro effects on S. mansoni. The in vitro bioassay of Punica granatum, (P. granatum) peels and leavesleavesSubject Category: Anatomical and Morphological Structures
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    extracts was carried out using ascending doses. Viability of worms and schistosomules was examined using negative (DMSO) and positive (PZQ) controls. This study revealed that P. granatum had significant effect on both adult S. mansoni worms and schistosomules reaching 100% death rate, 24 hours post exposure to extracted plant. Concerning the in vivo activity, P. granatum peels and leaves exhibited antischistosomal properties by the oral administration of either extract, in a dose of 800 mg/kg, 45 days post-infection and on 3 consecutive days; this dose was given following a pilot study to evaluate the highest safe dose. Parasitological parameters showed remarkable decrease manifested by high percentage of dead adult worm (77.30 and 72.2) with either leaves or peels extract respectively. The tissue egg load also revealed marked reduction in both liver and intestinal ova counts. The percentage reduction of adult worms reached (90.9 and 55.4) with extracted leaves or peels respectively, when P. granatum extracts were given 21 days post-infection using the same dose(800 mg/kg), denoting a high significant effect of leaves extracts on schistosomules. Electron microscopic examination of perfused adult worms, confirmed the parasitological results and revealed the effect of the methanolic extracts of P. granatum in inducing major ultrastructural alterations in the tegument and the male genital systems of the worms that lead to their death. In this study EM examination of bone marrow taken from S. mansoni infected mice, treated with either leaves or peels extracts of P. granatum showed complete degranulation of eosinophilseosinophilsSubject Category: Anatomical and Morphological Structures
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    and activated lymphocyteslymphocytesSubject Category: Anatomical and Morphological Structures
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    with extended projections and increased mitochondriamitochondriaSubject Category: Anatomical and Morphological Structures
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    in addition to activated monoblast with increased number of phagolysosomes. Since secretions of the different stages of S. mansoni have inhibitory effect on eosinophilic degranulation and monoblasts, as part of their parasite evasion mechanism, the apparent degranulation would indicate the decreased activity of S. mansoni. The leaves and peels extracts of Punica granatum could represent promising bioactive natural agents that deserve further investigation, with the aim of introducing novel anti-schistosomal agent.

    ISSN : 1991-8178

    URL : http://www.insipub.com/ajbas/2009/463...

    Record Number : 20103359507

    Publisher : INSInet Publications

    Location of publication : Faisalabad

    Country of publication : Pakistan

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : plantsplantsSubject Category: Organism Names
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    , Punica granatumpunica granatumSubject Category: Organism Names
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    , Schistosoma mansonischistosoma mansoniSubject Category: Organism Names
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    Descriptor(s) : anthelmintic propertiesanthelmintic propertiesSubject Category: Properties
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    , antimicrobial propertiesantimicrobial propertiesSubject Category: Properties
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    , antiparasitic agentsantiparasitic agentsSubject Category: Chemicals and Chemical Groups
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    , chemical compositionchemical compositionSubject Category: Properties
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    , eosinophilseosinophilsSubject Category: Anatomical and Morphological Structures
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    , fruitsfruitsSubject Category: Anatomical and Morphological Structures
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    , leavesleavesSubject Category: Anatomical and Morphological Structures
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    , lymphocyteslymphocytesSubject Category: Anatomical and Morphological Structures
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    , medicinal plantsmedicinal plantsSubject Category: Commodities and Products
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    , mitochondriamitochondriaSubject Category: Anatomical and Morphological Structures
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    , plant compositionplant compositionSubject Category: Properties
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    , plant extractsplant extractsSubject Category: Commodities and Products
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    , pomegranatespomegranatesSubject Category: Commodities and Products
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    , traditional medicinetraditional medicineSubject Category: Disciplines, Occupations and Industries
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    , traditional medicinestraditional medicinesSubject Category: Commodities and Products
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    Identifier(s) : anti-microbial properties, chemical constituents of plants, drug plants, eosinophil leukocytes, folk medicine, medicinal herbs, officinal plants, parasiticides, Strigeida

    Broader term(s) : PunicapunicaSubject Category: Organism Names
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    , LythraceaelythraceaeSubject Category: Organism Names
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    , MyrtalesmyrtalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , SchistosomaschistosomaSubject Category: Organism Names
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    , SchistosomatidaeschistosomatidaeSubject Category: Organism Names
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    , Digenea (Trematoda)digenea (trematoda)Subject Category: Organism Names
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    , TrematodatrematodaSubject Category: Organism Names
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    , PlatyhelminthesplatyhelminthesSubject Category: Organism Names
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    , invertebratesinvertebratesSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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