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  • The influence of cumulus cells during in vitro fertilization of buffalo (Bubalus bubalis) denuded oocytes that have undergone vitrification.

    Author(s) : Attanasio, L.Rosa, A. deBlasi, M. deNeglia, G.Zicarelli, L.Campanile, G.Gasparrini, B.

    Author Affiliation : DISCIZIA, Faculty of Veterinary Medicine, "Federico II" University, Via F. Delpino 1, 80137 Naples, Italy.

    Author Email : neglia@unina.it

    Journal article : Theriogenology 2010 Vol.74 No.8 pp.1504-1508 ref.25

    Abstract : The aim of this work was to evaluate whether providing a support of cumulus cells during IVF of buffalo denuded oocytesoocytesSubject Category: Anatomical and Morphological Structures
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    submitted to vitrificationvitrificationSubject Category: Techniques, Methodologies and Equipment
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    -warming enhances their fertilizing abilityfertilizing abilitySubject Category: Properties
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    . In vitroin vitroSubject Category: Techniques, Methodologies and Equipment
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    matured denuded oocytes were vitrified by Cryotop in 20% EG+20% of DMSO and 0.5 M sucrosesucroseSubject Category: Chemicals and Chemical Groups
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    and warmed into decreasing concentrations of sucrose (1.25 M-0.3M). Oocytes that survived vitrification were fertilized: (1) in the absence of a somatic support (DOs); (2) in the presence of bovine cumulus cells in suspension (DOs+susp); (3) on a bovine cumulus monolayer (DOs+monol); and (4) with intact bovine COCs in a 1:1 ratio (DOs+COCs). In vitro matured oocytes were fertilized and cultured to the blastocystblastocystSubject Category: Anatomical and Morphological Structures
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    stage as a control. An increased cleavage rate was obtained from DOs+COCs (60.9%) compared to DOs, DOs+susp (43.6 and 38.4, respectively; P<0.01) and DOs+monol (47.5%; P<0.05). Interestingly, cleavage rate of DOs+COCs was similar to that of fresh control oocytes (67.8%). However, development to blastocysts significantly decreased in all vitrification groups compared to the control (P<0.01). In conclusion the co-culture with intact COCs during IVF completely restores fertilizing capability of buffalo denuded vitrified oocytes, without improving blastocyst development.

    ISSN : 0093-691X

    DOI : 10.1016/j.theriogenology.2010.05.014

    Record Number : 20103374018

    Publisher : Elsevier

    Location of publication : New York

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : BubalusbubalusSubject Category: Organism Names
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    , buffaloesbuffaloesSubject Category: Organism Names
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    , cattlecattleSubject Category: Organism Names
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    Descriptor(s) : blastocystblastocystSubject Category: Anatomical and Morphological Structures
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    , culture techniquesculture techniquesSubject Category: Techniques, Methodologies and Equipment
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    , dimethyl sulfoxidedimethyl sulfoxideSubject Category: Chemicals and Chemical Groups
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    , embryosembryosSubject Category: Anatomical and Morphological Structures
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    , fertilizationfertilizationSubject Category: Natural Processes
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    , fertilizing abilityfertilizing abilitySubject Category: Properties
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    , in vitroin vitroSubject Category: Techniques, Methodologies and Equipment
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    , oocytesoocytesSubject Category: Anatomical and Morphological Structures
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    , sexual reproductionsexual reproductionSubject Category: Miscellaneous
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    , sucrosesucroseSubject Category: Chemicals and Chemical Groups
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    , vitrificationvitrificationSubject Category: Techniques, Methodologies and Equipment
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    Identifier(s) : cumulus cells, dimethyl sulphoxide, DMSO, fertilizing, saccharose

    Broader term(s) : BovidaebovidaeSubject Category: Organism Names
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    , ruminantsruminantsSubject Category: Organism Names
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    , ArtiodactylaartiodactylaSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , BubalusbubalusSubject Category: Organism Names
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    , BosbosSubject Category: Organism Names
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