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  • Influence of ovulation status, seasonality and embryo transfer method on development of cloned porcine embryos.

    Author(s) : Koo, O. J.Kang, J. T.Kwon, D. K.Park, H. J.Lee, B. C.

    Author Affiliation : Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul, Korea Republic.

    Author Email : bclee@snu.ac.kr

    Journal article : Reproduction in Domestic Animals 2010 Vol.45 No.5 pp.773-778 ref.33

    Abstract : To improve pig cloning efficiency, the present study evaluated the effect of ovulationovulationSubject Category: Natural Processes
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    status, seasonalityseasonalitySubject Category: Properties
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    and embryo transferembryo transferSubject Category: Miscellaneous
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    (ET) method on in vivo development of cloned porcine embryosembryosSubject Category: Anatomical and Morphological Structures
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    . Cloned embryos were transferred to surrogate mothersmothersSubject Category: People Groups
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    on the same day of somatic cell nuclear transfer. In pre-ovulation stage (PO), pregnancy ratepregnancy rateSubject Category: Properties
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    (PR) and delivery rate (DR) were 36.3% and 9.4%, respectively. In postovulation stage, 22.7% PR and 2.1% DR were recorded (both PR and DR are significantly higher in PO). When ET was performed during winterwinterSubject Category: Time Periods
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    winterSubject Category: Climate Related
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    (December-February), spring (March-May), summersummerSubject Category: Time Periods
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    summerSubject Category: Climate Related
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    (June-August) and autumn (September-November), the PRs were 13.4%, 37.3%, 24.6% and 51.0%, while DRs were 0%, 12.7%, 4.3% and 7.8%, respectively. The highest PRs were recorded in autumn groups. However, DRs were significantly lower in autumn (7.8%) group compared with spring (12.7%) group. The PR was the lowest and no pigletspigletsSubject Category: Organism Groups
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    were born in winter group, which might be because of the effect of low temperaturetemperatureSubject Category: Properties
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    during ET. To overcome the low PR in winter group, 0.25 ml straws were used for ET to minimize exposure time of embryos to ambient temperature. The strawstrawSubject Category: Commodities and Products
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    ET group showed significantly higher PR in the winter group (23. 9%) compared with the conventional catheter-loading group (7.7%). We suggest that using PO recipient and ET in spring is the best condition for pig cloning. In addition, alternative method to reduce cold shockcold shockSubject Category: Diseases, Disorders, and Symptoms
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    during ET in winter is necessary.

    ISSN : 0936-6768

    URL : http://onlinelibrary.wiley.com/.../pdf

    Record Number : 20103374666

    Publisher : Blackwell Publishing

    Location of publication : Berlin

    Country of publication : Germany

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : pigspigsSubject Category: Organism Names
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    Descriptor(s) : coldcoldSubject Category: Miscellaneous
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    , cold shockcold shockSubject Category: Diseases, Disorders, and Symptoms
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    , domestic animalsdomestic animalsSubject Category: Organism Groups
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    , effectseffectsSubject Category: Properties
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    , embryo transferembryo transferSubject Category: Miscellaneous
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    , embryosembryosSubject Category: Anatomical and Morphological Structures
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    , mothersmothersSubject Category: People Groups
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    , ovulationovulationSubject Category: Natural Processes
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    , pigletspigletsSubject Category: Organism Groups
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    , pregnancypregnancySubject Category: Miscellaneous
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    , pregnancy ratepregnancy rateSubject Category: Properties
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    , seasonalityseasonalitySubject Category: Properties
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    , sexual reproductionsexual reproductionSubject Category: Miscellaneous
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    , shockshockSubject Category: Diseases, Disorders, and Symptoms
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    , strawstrawSubject Category: Commodities and Products
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    , summersummerSubject Category: Climate Related
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    , temperaturetemperatureSubject Category: Properties
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    , winterwinterSubject Category: Climate Related
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    Identifier(s) : cloning, gestation, hogs, in vivo, swine

    Broader term(s) : Sus scrofasus scrofaSubject Category: Organism Names
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    , SussusSubject Category: Organism Names
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    , SuidaesuidaeSubject Category: Organism Names
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    , SuiformessuiformesSubject Category: Organism Names
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    , ArtiodactylaartiodactylaSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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