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  • Cloning of a very virulent plus, 686 strain of Marek's disease virus as a bacterial artificial chromosome.

    Author(s) : Reddy, S. M.Sun, A. J.Khan, O. A.Lee, L. F.Lupiani, B.

    Author Affiliation : College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.

    Author Email : blupiani@cvm.tamu.edu

    Journal article; Conference paper : Avian Diseases 2013 Vol.57 No.2s1 pp.469-473

    Conference Title : Ninth International Symposium on Marek's Disease and Avian Herpesviruses, Berlin, Germany, 24-28 June 2012.

    Abstract : Bacterial artificial chromosome (BAC) vectorsvectorsSubject Category: Miscellaneous
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    were first developed to facilitate propagation and manipulation of large DNA fragments. This technology was later used to clone full-length genomesgenomesSubject Category: Miscellaneous
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    of large DNA virusesdna virusesSubject Category: Organism Names
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    to study viral gene function. Marek's disease virus (MDV) is a highly oncogenic herpesvirus that causes rapid induction of T-cell lymphomas in chickens. Based on the virus's ability to cause disease in vaccinated chickens, MDV strains are classified into pathotypes, with the most virulent strains belonging to the very virulent plus (vv+) pathotype. Here we report the construction of BAC clones of 686 (686-BAC), a vv+ strain of MDV. TransfectiontransfectionSubject Category: Miscellaneous
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    of DNA isolated from two independent clones into duck embryo fibroblastsfibroblastsSubject Category: Anatomical and Morphological Structures
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    resulted in recovery of infectious virus. PathogenesispathogenesisSubject Category: Miscellaneous
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    studies showed that the BAC-derived 686 viruses were more virulent than Md5, a vv strain of MDV. With the use of a two-step red-mediated mutagenesismutagenesisSubject Category: Miscellaneous
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    process, both copies of viral interleukin 8interleukin 8Subject Category: Chemicals and Chemical Groups
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    (vIL-8) were deleted from the MDV genome, showing that 686-BACs were amenable to mutagenesis techniquestechniquesSubject Category: Techniques, Methodologies and Equipment
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    . The generation of BAC clones from a vv+ strain of MDV is a significant step toward understanding molecular basis of MDV pathogenesis.

    ISSN : 0005-2086

    DOI : 10.1637/10444-110412-ResNote.1

    Record Number : 20133239010

    Publisher : American Association of Avian Pathologists

    Location of publication : Athens

    Country of publication : USA

    Language of text : English

    Language of summary : Spanish

    Indexing terms for this abstract:

    Organism descriptor(s) : birdsbirdsSubject Category: Organism Names
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    , DNA Virusesdna virusesSubject Category: Organism Names
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    , ducksducksSubject Category: Organism Names
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    , fowlsfowlsSubject Category: Organism Names
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    , Gallid herpesvirus 2gallid herpesvirus 2Subject Category: Organism Names
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    , HerpesviridaeherpesviridaeSubject Category: Organism Names
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    , MardivirusmardivirusSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    Descriptor(s) : animal diseasesanimal diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , chromosomeschromosomesSubject Category: Anatomical and Morphological Structures
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    , DNAdnaSubject Category: Chemicals and Chemical Groups
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    , embryosembryosSubject Category: Anatomical and Morphological Structures
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    , fibroblastsfibroblastsSubject Category: Anatomical and Morphological Structures
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    , genomesgenomesSubject Category: Miscellaneous
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    , infectious diseasesinfectious diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , interleukin 8interleukin 8Subject Category: Chemicals and Chemical Groups
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    , interleukinsinterleukinsSubject Category: Chemicals and Chemical Groups
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    , lymphomalymphomaSubject Category: Diseases, Disorders, and Symptoms
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    , mutagenesismutagenesisSubject Category: Miscellaneous
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    , neoplasmsneoplasmsSubject Category: Diseases, Disorders, and Symptoms
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    , pathogenesispathogenesisSubject Category: Miscellaneous
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    , poultrypoultrySubject Category: Organism Groups
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    , researchresearchSubject Category: Disciplines, Occupations and Industries
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    , T lymphocytest lymphocytesSubject Category: Anatomical and Morphological Structures
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    , techniquestechniquesSubject Category: Techniques, Methodologies and Equipment
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    , transfectiontransfectionSubject Category: Miscellaneous
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    , vectorsvectorsSubject Category: Miscellaneous
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    , viral diseasesviral diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , virulencevirulenceSubject Category: Miscellaneous
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    Identifier(s) : cancers, chickens, cloning, communicable diseases, deoxyribonucleic acid, domesticated birds, Marek's disease virus, studies, T cells, viral infections

    Broader term(s) : vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    , AnatidaeanatidaeSubject Category: Organism Names
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    , AnseriformesanseriformesSubject Category: Organism Names
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    , birdsbirdsSubject Category: Organism Names
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    , Gallus gallusgallus gallusSubject Category: Organism Names
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    , GallusgallusSubject Category: Organism Names
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    , PhasianidaephasianidaeSubject Category: Organism Names
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    , GalliformesgalliformesSubject Category: Organism Names
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    , HerpesviralesherpesviralesSubject Category: Organism Names
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    , dsDNA Virusesdsdna virusesSubject Category: Organism Names
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    , DNA Virusesdna virusesSubject Category: Organism Names
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    , AlphaherpesvirinaealphaherpesvirinaeSubject Category: Organism Names
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    , HerpesviridaeherpesviridaeSubject Category: Organism Names
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    , MardivirusmardivirusSubject Category: Organism Names
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