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Author(s) :
Jhuma Datta
;
Nand Lal
Author Affiliation :
Department of Life Sciences, C.S.J.M. University, Kanpur 208 024, India.
Author Email :
nl_pr@yahoo.co.in
Journal article
:
Journal of Microbiology, Biotechnology and Food Sciences
2013
Vol.2
No.6
pp.2392-2397
ref.41
Abstract :
Genetic diversitygenetic diversitySubject Category: Properties
see more details in Chickpea wilt pathogen has been characterized using 14 isolates of
Fusarium oxysporum f. sp.
ciceri (foc) collected from major pulse growing regions of
IndiaindiaSubject Category: Geographic Entities
see more details. Out of 247 bands produced by 24
Random Amplified Polymorphic DNArandom amplified polymorphic dnaSubject Category: Techniques, Methodologies and Equipment
see more details (RAPD) primers in Foc isolates, 210 (85%) were polymorphic. A maximum of 14 amplicons were generated by primer OPF 05 whereas minimum 7 amplicons were generated by primer K7. A total of 24
allelesallelesSubject Category: Miscellaneous
see more details were produced by twelve simple sequence repeat (SSR) primers with an average of two alleles per marker in foc isolates. The maximum number of 4 alleles was obtained with primer SSR 12. SSR amplicon size ranged from 100 to 400 bp. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA)
cluster analysiscluster analysisSubject Category: Techniques, Methodologies and Equipment
see more details based on RAPD and SSR profiles grouped the fourteen foc isolates into four major clusters. The universal Inter Transcribed Spacer (ITS) primer pair amplified 630 bp bands in all fourteen foc isolates while significant length polymorphism was obtained only when analysed by restriction digestion with EcoRI and MspI enzymes. The cluster analysis of ITS-RFLP grouped all 14 Foc isolates into three major clusters. The cluster analysis using RAPD, SSR and ITS-RFLP markers show the grouping of
Fusarium isolates strictly according to their cultural characteristics and degree of pathogenicity and not the geographical origin. This information will be helpful for pathologists and plant breeders to design effective resistance breeding programs in chickpea taking into account the diversity in wilt pathogen.
ISSN :
1338-5178
URL :
http://www.jmbfs.org/wp-content/uploa...
Record Number :
20133239287
Publisher :
Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture
Location of publication :
Nitra
Country of publication :
Slovakia
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
fungifungiSubject Category: Organism Names
see more details, Fusarium oxysporum f.sp. cicerisfusarium oxysporum f.sp. cicerisSubject Category: Organism Names
see more details
Descriptor(s) :
allelesallelesSubject Category: Miscellaneous
see more details, cluster analysiscluster analysisSubject Category: Techniques, Methodologies and Equipment
see more details, fungal diseasesfungal diseasesSubject Category: Diseases, Disorders, and Symptoms
see more details, genetic diversitygenetic diversitySubject Category: Properties
see more details, genetic markersgenetic markersSubject Category: Miscellaneous
see more details, genetic polymorphismgenetic polymorphismSubject Category: Properties
see more details, genetic variationgenetic variationSubject Category: Properties
see more details, pathogenspathogensSubject Category: Organism Groups
see more details, plant diseasesplant diseasesSubject Category: Diseases, Disorders, and Symptoms
see more details, plant pathogenic fungiplant pathogenic fungiSubject Category: Organism Groups
see more details, plant pathogensplant pathogensSubject Category: Organism Groups
see more details, population geneticspopulation geneticsSubject Category: Miscellaneous
see more details, racesracesSubject Category: Organism Groups
see more details, random amplified polymorphic DNArandom amplified polymorphic dnaSubject Category: Techniques, Methodologies and Equipment
see more details, restriction fragment length polymorphismrestriction fragment length polymorphismSubject Category: Properties
see more details, simple sequence repeatssimple sequence repeatsSubject Category: Miscellaneous
see more details
Identifier(s) :
fungus, genetic variability, genotypic variability, genotypic variation, Mysore, phytopathogenic fungi, phytopathogens, plant-pathogenic fungi, RAPD, RFLP
Geographical Location(s) :
Andhra Pradeshandhra pradeshSubject Category: Geographic Entities
see more details, ChhattisgarhchhattisgarhSubject Category: Geographic Entities
see more details, GujaratgujaratSubject Category: Geographic Entities
see more details, IndiaindiaSubject Category: Geographic Entities
see more details, KarnatakakarnatakaSubject Category: Geographic Entities
see more details, Madhya Pradeshmadhya pradeshSubject Category: Geographic Entities
see more details, MaharashtramaharashtraSubject Category: Geographic Entities
see more details, Uttar Pradeshuttar pradeshSubject Category: Geographic Entities
see more details
Broader term(s) :
eukaryoteseukaryotesSubject Category: Organism Names
see more details, Fusarium oxysporumfusarium oxysporumSubject Category: Organism Names
see more details, FusariumfusariumSubject Category: Organism Names
see more details, NectriaceaenectriaceaeSubject Category: Organism Names
see more details, HypocrealeshypocrealesSubject Category: Organism Names
see more details, SordariomycetessordariomycetesSubject Category: Organism Names
see more details, PezizomycotinapezizomycotinaSubject Category: Organism Names
see more details, AscomycotaascomycotaSubject Category: Organism Names
see more details, fungifungiSubject Category: Organism Names
see more details, IndiaindiaSubject Category: Geographic Entities
see more details, Commonwealth of Nationscommonwealth of nationsSubject Category: Institutions and Organisations
see more details, Developing Countriesdeveloping countriesSubject Category: Miscellaneous
see more details, South Asiasouth asiaSubject Category: Geographic Entities
see more details, AsiaasiaSubject Category: Geographic Entities
see more details