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  • Genetic variability assessment of Fusarium wilt pathogen races affecting chickpea using molecular markers.

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    Author(s) : Jhuma DattaNand Lal

    Author Affiliation : Department of Life Sciences, C.S.J.M. University, Kanpur 208 024, India.

    Author Email : nl_pr@yahoo.co.in

    Journal article : Journal of Microbiology, Biotechnology and Food Sciences 2013 Vol.2 No.6 pp.2392-2397 ref.41

    Abstract : Genetic diversitygenetic diversitySubject Category: Properties
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    in Chickpea wilt pathogen has been characterized using 14 isolates of Fusarium oxysporum f. sp. ciceri (foc) collected from major pulse growing regions of IndiaindiaSubject Category: Geographic Entities
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    . Out of 247 bands produced by 24 Random Amplified Polymorphic DNArandom amplified polymorphic dnaSubject Category: Techniques, Methodologies and Equipment
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    (RAPD) primers in Foc isolates, 210 (85%) were polymorphic. A maximum of 14 amplicons were generated by primer OPF 05 whereas minimum 7 amplicons were generated by primer K7. A total of 24 allelesallelesSubject Category: Miscellaneous
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    were produced by twelve simple sequence repeat (SSR) primers with an average of two alleles per marker in foc isolates. The maximum number of 4 alleles was obtained with primer SSR 12. SSR amplicon size ranged from 100 to 400 bp. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysiscluster analysisSubject Category: Techniques, Methodologies and Equipment
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    based on RAPD and SSR profiles grouped the fourteen foc isolates into four major clusters. The universal Inter Transcribed Spacer (ITS) primer pair amplified 630 bp bands in all fourteen foc isolates while significant length polymorphism was obtained only when analysed by restriction digestion with EcoRI and MspI enzymes. The cluster analysis of ITS-RFLP grouped all 14 Foc isolates into three major clusters. The cluster analysis using RAPD, SSR and ITS-RFLP markers show the grouping of Fusarium isolates strictly according to their cultural characteristics and degree of pathogenicity and not the geographical origin. This information will be helpful for pathologists and plant breeders to design effective resistance breeding programs in chickpea taking into account the diversity in wilt pathogen.

    ISSN : 1338-5178

    URL : http://www.jmbfs.org/wp-content/uploa...

    Record Number : 20133239287

    Publisher : Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture

    Location of publication : Nitra

    Country of publication : Slovakia

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : fungifungiSubject Category: Organism Names
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    , Fusarium oxysporum f.sp. cicerisfusarium oxysporum f.sp. cicerisSubject Category: Organism Names
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    Descriptor(s) : allelesallelesSubject Category: Miscellaneous
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    , cluster analysiscluster analysisSubject Category: Techniques, Methodologies and Equipment
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    , fungal diseasesfungal diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , genetic diversitygenetic diversitySubject Category: Properties
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    , genetic markersgenetic markersSubject Category: Miscellaneous
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    , genetic polymorphismgenetic polymorphismSubject Category: Properties
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    , genetic variationgenetic variationSubject Category: Properties
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    , pathogenspathogensSubject Category: Organism Groups
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    , plant diseasesplant diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , plant pathogenic fungiplant pathogenic fungiSubject Category: Organism Groups
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    , plant pathogensplant pathogensSubject Category: Organism Groups
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    , population geneticspopulation geneticsSubject Category: Miscellaneous
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    , racesracesSubject Category: Organism Groups
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    , random amplified polymorphic DNArandom amplified polymorphic dnaSubject Category: Techniques, Methodologies and Equipment
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    , restriction fragment length polymorphismrestriction fragment length polymorphismSubject Category: Properties
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    , simple sequence repeatssimple sequence repeatsSubject Category: Miscellaneous
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    Identifier(s) : fungus, genetic variability, genotypic variability, genotypic variation, Mysore, phytopathogenic fungi, phytopathogens, plant-pathogenic fungi, RAPD, RFLP

    Geographical Location(s) : Andhra Pradeshandhra pradeshSubject Category: Geographic Entities
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    , ChhattisgarhchhattisgarhSubject Category: Geographic Entities
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    , GujaratgujaratSubject Category: Geographic Entities
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    , IndiaindiaSubject Category: Geographic Entities
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    , KarnatakakarnatakaSubject Category: Geographic Entities
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    , Madhya Pradeshmadhya pradeshSubject Category: Geographic Entities
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    , MaharashtramaharashtraSubject Category: Geographic Entities
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    , Uttar Pradeshuttar pradeshSubject Category: Geographic Entities
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    Broader term(s) : eukaryoteseukaryotesSubject Category: Organism Names
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    , Fusarium oxysporumfusarium oxysporumSubject Category: Organism Names
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    , FusariumfusariumSubject Category: Organism Names
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    , NectriaceaenectriaceaeSubject Category: Organism Names
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    , HypocrealeshypocrealesSubject Category: Organism Names
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    , SordariomycetessordariomycetesSubject Category: Organism Names
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    , PezizomycotinapezizomycotinaSubject Category: Organism Names
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    , AscomycotaascomycotaSubject Category: Organism Names
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    , fungifungiSubject Category: Organism Names
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    , IndiaindiaSubject Category: Geographic Entities
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    , Commonwealth of Nationscommonwealth of nationsSubject Category: Institutions and Organisations
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    , Developing Countriesdeveloping countriesSubject Category: Miscellaneous
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    , South Asiasouth asiaSubject Category: Geographic Entities
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    , AsiaasiaSubject Category: Geographic Entities
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