Author(s) :
Duan JiangYan
;
Tian Xue
;
Jia ZhenHu
Author Affiliation :
College of Life Science, Shanxi Normal University, Linfen, China.
Author Email :
jiaxueshi@163.com
Journal article
:
American Journal of Plant Sciences
2013
Vol.4
No.6
pp.1227-1232
ref.14
Abstract :
WheatwheatSubject Category: Commodities and Products
see more details (
Triticum aestivumtriticum aestivumSubject Category: Organism Names
see more details), as a kind of important economic crop cultured in the
Northern Chinanorthern chinaSubject Category: Geographic Entities
see more details, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the proteins of wheat (
Jin mai NO.8)
leavesleavesSubject Category: Anatomical and Morphological Structures
see more details, which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: (1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day; (2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day; (3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein; SOD;
CalmodulincalmodulinSubject Category: Chemicals and Chemical Groups
see more details. The result indicates wheat could improve
genesgenesSubject Category: Miscellaneous
see more details encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.
ISSN :
2158-2742
DOI :
10.4236/ajps.2013.46150
Record Number :
20133240641
Publisher :
Scientific Research Publishing
Location of publication :
Irvine
Country of publication :
USA
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
TriticumtriticumSubject Category: Organism Names
see more details, Triticum aestivumtriticum aestivumSubject Category: Organism Names
see more details
Descriptor(s) :
binding proteinsbinding proteinsSubject Category: Chemicals and Chemical Groups
see more details, calmodulincalmodulinSubject Category: Chemicals and Chemical Groups
see more details, enzymesenzymesSubject Category: Chemicals and Chemical Groups
see more details, genesgenesSubject Category: Miscellaneous
see more details, leavesleavesSubject Category: Anatomical and Morphological Structures
see more details, plant proteinsplant proteinsSubject Category: Chemicals and Chemical Groups
see more details, proteomicsproteomicsSubject Category: Disciplines, Occupations and Industries
see more details, ribulose-bisphosphate carboxylaseribulose-bisphosphate carboxylaseSubject Category: Chemicals and Chemical Groups
see more details, superoxide dismutasesuperoxide dismutaseSubject Category: Chemicals and Chemical Groups
see more details, ultraviolet radiationultraviolet radiationSubject Category: Miscellaneous
see more details, wheatwheatSubject Category: Commodities and Products
see more details
Identifier(s) :
carrier proteins, People's Republic of China, ribulose-1,5-diphosphate carboxylase, ribulose-1,5-diphosphate oxygenase
Geographical Location(s) :
ChinachinaSubject Category: Geographic Entities
see more details, Northern Chinanorthern chinaSubject Category: Geographic Entities
see more details
Broader term(s) :
PoaceaepoaceaeSubject Category: Organism Names
see more details, PoalespoalesSubject Category: Organism Names
see more details, commelinidscommelinidsSubject Category: Organism Names
see more details, monocotyledonsmonocotyledonsSubject Category: Organism Names
see more details, angiospermsangiospermsSubject Category: Organism Names
see more details, SpermatophytaspermatophytaSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details, TriticumtriticumSubject Category: Organism Names
see more details, APEC countriesapec countriesSubject Category: Institutions and Organisations
see more details, Developing Countriesdeveloping countriesSubject Category: Miscellaneous
see more details, East Asiaeast asiaSubject Category: Geographic Entities
see more details, AsiaasiaSubject Category: Geographic Entities
see more details, ChinachinaSubject Category: Geographic Entities
see more details