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  • Proteomics uncovers a role for enhanced ultraviolet-B radiation on wheat leaves.

    Author(s) : Duan JiangYanTian XueJia ZhenHu

    Author Affiliation : College of Life Science, Shanxi Normal University, Linfen, China.

    Author Email : jiaxueshi@163.com

    Journal article : American Journal of Plant Sciences 2013 Vol.4 No.6 pp.1227-1232 ref.14

    Abstract : WheatwheatSubject Category: Commodities and Products
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    (Triticum aestivumtriticum aestivumSubject Category: Organism Names
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    ), as a kind of important economic crop cultured in the Northern Chinanorthern chinaSubject Category: Geographic Entities
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    , is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the proteins of wheat (Jin mai NO.8) leavesleavesSubject Category: Anatomical and Morphological Structures
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    , which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: (1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day; (2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day; (3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein; SOD; CalmodulincalmodulinSubject Category: Chemicals and Chemical Groups
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    . The result indicates wheat could improve genesgenesSubject Category: Miscellaneous
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    encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.

    ISSN : 2158-2742

    DOI : 10.4236/ajps.2013.46150

    Record Number : 20133240641

    Publisher : Scientific Research Publishing

    Location of publication : Irvine

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : TriticumtriticumSubject Category: Organism Names
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    , Triticum aestivumtriticum aestivumSubject Category: Organism Names
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    Descriptor(s) : binding proteinsbinding proteinsSubject Category: Chemicals and Chemical Groups
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    , calmodulincalmodulinSubject Category: Chemicals and Chemical Groups
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , genesgenesSubject Category: Miscellaneous
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    , leavesleavesSubject Category: Anatomical and Morphological Structures
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    , plant proteinsplant proteinsSubject Category: Chemicals and Chemical Groups
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    , proteomicsproteomicsSubject Category: Disciplines, Occupations and Industries
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    , ribulose-bisphosphate carboxylaseribulose-bisphosphate carboxylaseSubject Category: Chemicals and Chemical Groups
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    , superoxide dismutasesuperoxide dismutaseSubject Category: Chemicals and Chemical Groups
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    , ultraviolet radiationultraviolet radiationSubject Category: Miscellaneous
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    , wheatwheatSubject Category: Commodities and Products
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    Identifier(s) : carrier proteins, People's Republic of China, ribulose-1,5-diphosphate carboxylase, ribulose-1,5-diphosphate oxygenase

    Geographical Location(s) : ChinachinaSubject Category: Geographic Entities
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    , Northern Chinanorthern chinaSubject Category: Geographic Entities
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    Broader term(s) : PoaceaepoaceaeSubject Category: Organism Names
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    , PoalespoalesSubject Category: Organism Names
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    , commelinidscommelinidsSubject Category: Organism Names
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    , monocotyledonsmonocotyledonsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , TriticumtriticumSubject Category: Organism Names
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    , APEC countriesapec countriesSubject Category: Institutions and Organisations
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    , Developing Countriesdeveloping countriesSubject Category: Miscellaneous
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    , East Asiaeast asiaSubject Category: Geographic Entities
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    , AsiaasiaSubject Category: Geographic Entities
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    , ChinachinaSubject Category: Geographic Entities
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