Author(s) :
Legrand, S.
;
Marque, G.
;
Blassiau, C.
;
Bluteau, A.
;
Canoy, A. S.
;
Fontaine, V.
;
Jaminon, O.
;
Bahrman, N.
;
Mautord, J.
;
Morin, J.
;
Petit, A.
;
Baranger, A.
;
Rivière, N.
;
Wilmer, J.
;
Delbreil, B.
;
Lejeune-Hénaut, I.
Author Affiliation :
Université Lille 1, UMR SADV 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, Université Lille Nord de France, Lille 1, SN2, F-59650 Villeneuve d'Ascq, France.
Author Email :
Sylvain.legrand@univ-lille1.fr
Journal article
:
Journal of Plant Physiology
2013
Vol.170
No.13
pp.1148-1157
ref.many
Abstract :
Cold stresscold stressSubject Category: Miscellaneous
see more details affects plant growth and development. In order to better understand the responses to cold (
chillingchillingSubject Category: Techniques, Methodologies and Equipment
see more details or
freezingfreezingSubject Category: Techniques, Methodologies and Equipment
see more details tolerance), we used two contrasted pea lines. Following a chilling period, the Champagne line becomes tolerant to frost whereas the Terese line remains sensitive. Four suppression subtractive hybridisation libraries were obtained using mRNAs isolated from pea
genotypesgenotypesSubject Category: Miscellaneous
see more details Champagne and Terese. Using quantitative
polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
see more details (qPCR) performed on 159
genesgenesSubject Category: Miscellaneous
see more details, 43 and 54 genes were identified as differentially expressed at the initial time point and during the time course study, respectively. Molecular markers were developed from the differentially expressed genes and were genotyped on a population of 164 RILs derived from a cross between Champagne and Terese. We identified 5 candidate genes colocalizing with 3 different frost
damagedamageSubject Category: Miscellaneous
see more details quantitative trait lociquantitative trait lociSubject Category: Miscellaneous
see more details (QTL) intervals and a protein quantity locus (PQL) rich region previously reported. This investigation revealed the role of constitutive differences between both genotypes in the cold responses, in particular with genes related to glycine
degradationdegradationSubject Category: Miscellaneous
see more details pathway that could confer to Champagne a better frost tolerance. We showed that freezing tolerance involves a decrease of expression of genes related to
photosynthesisphotosynthesisSubject Category: Natural Processes
see more details and the expression of a gene involved in the
productionproductionSubject Category: Miscellaneous
see more details of
cysteinecysteineSubject Category: Chemicals and Chemical Groups
see more details and
methioninemethionineSubject Category: Chemicals and Chemical Groups
see more details that could act as cryoprotectant molecules. Although it remains to be confirmed, this study could also reveal the involvement of the jasmonate pathway in the cold responses, since we observed that two genes related to this pathway were mapped in a frost damage QTL interval and in a PQL rich region interval, respectively.
ISSN :
0176-1617
DOI :
10.1016/j.jplph.2013.03.014
Record Number :
20133310535
Publisher :
Elsevier GmbH
Location of publication :
München
Country of publication :
Germany
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
PisumpisumSubject Category: Organism Names
see more details, Pisum sativumpisum sativumSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details
Descriptor(s) :
breeding methodsbreeding methodsSubject Category: Techniques, Methodologies and Equipment
see more details, chillingchillingSubject Category: Techniques, Methodologies and Equipment
see more details, cold stresscold stressSubject Category: Miscellaneous
see more details, crossescrossesSubject Category: Miscellaneous
see more details, cysteinecysteineSubject Category: Chemicals and Chemical Groups
see more details, damagedamageSubject Category: Miscellaneous
see more details, degradationdegradationSubject Category: Miscellaneous
see more details, effectseffectsSubject Category: Properties
see more details, freezingfreezingSubject Category: Techniques, Methodologies and Equipment
see more details, frostfrostSubject Category: Climate Related
see more details, frost injuryfrost injurySubject Category: Miscellaneous
see more details, frost resistancefrost resistanceSubject Category: Properties
see more details, gene expressiongene expressionSubject Category: Miscellaneous
see more details, genesgenesSubject Category: Miscellaneous
see more details, genetic markersgenetic markersSubject Category: Miscellaneous
see more details, genotypesgenotypesSubject Category: Miscellaneous
see more details, growthgrowthSubject Category: Natural Processes
see more details, hybridizationhybridizationSubject Category: Techniques, Methodologies and Equipment
see more details, injuriesinjuriesSubject Category: Miscellaneous
see more details, locilociSubject Category: Miscellaneous
see more details, methioninemethionineSubject Category: Chemicals and Chemical Groups
see more details, molecular geneticsmolecular geneticsSubject Category: Miscellaneous
see more details, peaspeasSubject Category: Commodities and Products
see more details, photosynthesisphotosynthesisSubject Category: Natural Processes
see more details, plant developmentplant developmentSubject Category: Natural Processes
see more details, plant growth regulatorsplant growth regulatorsSubject Category: Chemicals and Chemical Groups
see more details, plant physiologyplant physiologySubject Category: Miscellaneous
see more details, polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
see more details, productionproductionSubject Category: Miscellaneous
see more details, quantitative trait lociquantitative trait lociSubject Category: Miscellaneous
see more details, quantitative traitsquantitative traitsSubject Category: Properties
see more details, stressstressSubject Category: Miscellaneous
see more details
Identifier(s) :
biochemical genetics, carbon assimilation, carbon dioxide fixation, frost damage, jasmonates, PCR, pea, plant growth substances, plant hormones
Broader term(s) :
PapilionoideaepapilionoideaeSubject Category: Organism Names
see more details, FabaceaefabaceaeSubject Category: Organism Names
see more details, FabalesfabalesSubject Category: Organism Names
see more details, eudicotseudicotsSubject Category: Organism Names
see more details, angiospermsangiospermsSubject Category: Organism Names
see more details, SpermatophytaspermatophytaSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details, PisumpisumSubject Category: Organism Names
see more details