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  • Combining gene expression and genetic analyses to identify candidate genes involved in cold responses in pea.

    Author(s) : Legrand, S.Marque, G.Blassiau, C.Bluteau, A.Canoy, A. S.Fontaine, V.Jaminon, O.Bahrman, N.Mautord, J.Morin, J.Petit, A.Baranger, A.Rivière, N.Wilmer, J.Delbreil, B.Lejeune-Hénaut, I.

    Author Affiliation : Université Lille 1, UMR SADV 1281, Stress Abiotiques et Différenciation des Végétaux cultivés, Université Lille Nord de France, Lille 1, SN2, F-59650 Villeneuve d'Ascq, France.

    Author Email : Sylvain.legrand@univ-lille1.fr

    Journal article : Journal of Plant Physiology 2013 Vol.170 No.13 pp.1148-1157 ref.many

    Abstract : Cold stresscold stressSubject Category: Miscellaneous
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    affects plant growth and development. In order to better understand the responses to cold (chillingchillingSubject Category: Techniques, Methodologies and Equipment
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    or freezingfreezingSubject Category: Techniques, Methodologies and Equipment
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    tolerance), we used two contrasted pea lines. Following a chilling period, the Champagne line becomes tolerant to frost whereas the Terese line remains sensitive. Four suppression subtractive hybridisation libraries were obtained using mRNAs isolated from pea genotypesgenotypesSubject Category: Miscellaneous
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    Champagne and Terese. Using quantitative polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
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    (qPCR) performed on 159 genesgenesSubject Category: Miscellaneous
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    , 43 and 54 genes were identified as differentially expressed at the initial time point and during the time course study, respectively. Molecular markers were developed from the differentially expressed genes and were genotyped on a population of 164 RILs derived from a cross between Champagne and Terese. We identified 5 candidate genes colocalizing with 3 different frost damagedamageSubject Category: Miscellaneous
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    quantitative trait lociquantitative trait lociSubject Category: Miscellaneous
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    (QTL) intervals and a protein quantity locus (PQL) rich region previously reported. This investigation revealed the role of constitutive differences between both genotypes in the cold responses, in particular with genes related to glycine degradationdegradationSubject Category: Miscellaneous
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    pathway that could confer to Champagne a better frost tolerance. We showed that freezing tolerance involves a decrease of expression of genes related to photosynthesisphotosynthesisSubject Category: Natural Processes
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    and the expression of a gene involved in the productionproductionSubject Category: Miscellaneous
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    of cysteinecysteineSubject Category: Chemicals and Chemical Groups
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    and methioninemethionineSubject Category: Chemicals and Chemical Groups
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    that could act as cryoprotectant molecules. Although it remains to be confirmed, this study could also reveal the involvement of the jasmonate pathway in the cold responses, since we observed that two genes related to this pathway were mapped in a frost damage QTL interval and in a PQL rich region interval, respectively.

    ISSN : 0176-1617

    DOI : 10.1016/j.jplph.2013.03.014

    Record Number : 20133310535

    Publisher : Elsevier GmbH

    Location of publication : München

    Country of publication : Germany

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : PisumpisumSubject Category: Organism Names
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    , Pisum sativumpisum sativumSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    Descriptor(s) : breeding methodsbreeding methodsSubject Category: Techniques, Methodologies and Equipment
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    , chillingchillingSubject Category: Techniques, Methodologies and Equipment
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    , cold stresscold stressSubject Category: Miscellaneous
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    , crossescrossesSubject Category: Miscellaneous
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    , cysteinecysteineSubject Category: Chemicals and Chemical Groups
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    , damagedamageSubject Category: Miscellaneous
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    , degradationdegradationSubject Category: Miscellaneous
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    , effectseffectsSubject Category: Properties
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    , freezingfreezingSubject Category: Techniques, Methodologies and Equipment
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    , frostfrostSubject Category: Climate Related
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    , frost injuryfrost injurySubject Category: Miscellaneous
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    , frost resistancefrost resistanceSubject Category: Properties
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    , gene expressiongene expressionSubject Category: Miscellaneous
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    , genesgenesSubject Category: Miscellaneous
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    , genetic markersgenetic markersSubject Category: Miscellaneous
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    , genotypesgenotypesSubject Category: Miscellaneous
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    , growthgrowthSubject Category: Natural Processes
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    , hybridizationhybridizationSubject Category: Techniques, Methodologies and Equipment
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    , injuriesinjuriesSubject Category: Miscellaneous
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    , locilociSubject Category: Miscellaneous
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    , methioninemethionineSubject Category: Chemicals and Chemical Groups
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    , molecular geneticsmolecular geneticsSubject Category: Miscellaneous
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    , peaspeasSubject Category: Commodities and Products
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    , photosynthesisphotosynthesisSubject Category: Natural Processes
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    , plant developmentplant developmentSubject Category: Natural Processes
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    , plant growth regulatorsplant growth regulatorsSubject Category: Chemicals and Chemical Groups
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    , plant physiologyplant physiologySubject Category: Miscellaneous
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    , polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
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    , productionproductionSubject Category: Miscellaneous
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    , quantitative trait lociquantitative trait lociSubject Category: Miscellaneous
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    , quantitative traitsquantitative traitsSubject Category: Properties
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    , stressstressSubject Category: Miscellaneous
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    Identifier(s) : biochemical genetics, carbon assimilation, carbon dioxide fixation, frost damage, jasmonates, PCR, pea, plant growth substances, plant hormones

    Broader term(s) : PapilionoideaepapilionoideaeSubject Category: Organism Names
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    , FabaceaefabaceaeSubject Category: Organism Names
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    , FabalesfabalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , PisumpisumSubject Category: Organism Names
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