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  • In vitro evaluation of the antiviral activity of extracts of Gynostemma pentaphyllum against poliomyelitis virus.

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    Author(s) : Okoye, E. L.

    Author Affiliation : Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University, PMB 5025, Awka, Anambra State, Nigeria.

    Author Email : ebyemma2006@yahoo.com

    Journal article : International Journal of Agriculture and Biosciences 2013 Vol.2 No.2 pp.64-67 ref.16

    Abstract : Antiviral activity of the crude extracts of Gynostemma pentaphyllumgynostemma pentaphyllumSubject Category: Organism Names
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    on PoliomyelitispoliomyelitisSubject Category: Diseases, Disorders, and Symptoms
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    virus was evaluated. The dried and powdered leaves of the plant - Gynostemma pentaphyllum were extracted with methanol, water and petroleum ether using standard methods. The cytotoxicity of the extracts was evaluated using the end-point Cytopathic effect assay on L20B cell linescell linesSubject Category: Organism Groups
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    . Phytochemical evaluations of the extracts were also carried out. The Poliovirus was titrated and determination of the 50% tissue culturetissue cultureSubject Category: Techniques, Methodologies and Equipment
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    infective dose (TCID50) done on L20B cells. The 50% tissue culture infective dose was later calculated using standard methods. The antiviral propertiesantiviral propertiesSubject Category: Properties
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    were determined against the three sero-types of Poliovirus (SL1, SL2 and SL3) using the end-point Cytopathic effect assay on L20B cell lines. Phytochemical analysis of the extracts revealed the presence of saponinssaponinsSubject Category: Chemicals and Chemical Groups
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    , alkaloids, glycosidesglycosidesSubject Category: Chemicals and Chemical Groups
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    , tanninstanninsSubject Category: Chemicals and Chemical Groups
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    , flavonoidsflavonoidsSubject Category: Chemicals and Chemical Groups
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    , carbohydratescarbohydratesSubject Category: Chemicals and Chemical Groups
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    , reducing sugar, resinsresinsSubject Category: Chemicals and Chemical Groups
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    , fatsfatsSubject Category: Chemicals and Chemical Groups
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    and oil, acidic compounds and proteins. Poliomyelitis viral infectivity was inhibited by the extracts giving a range of specificity indices of 25-150. This shows that the extracts selectively inhibited the virus and that their activity against the virus was not just a consequence of toxicity to the cells. The research has shown that the plant possesses potent antiviral potentials and could serve as a possible source of lead antiviral drug against Poliomyelitis since the disease has no known drug for treatment.

    ISSN : 2305-6622

    URL : http://www.ijagbio.com/.../64-67.pdf

    Record Number : 20133328543

    Publisher : Faculty of Agriculture, Nnamdi Azikiwe University

    Location of publication : Anambra

    Country of publication : Nigeria

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Gynostemma pentaphyllumgynostemma pentaphyllumSubject Category: Organism Names
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    Descriptor(s) : antiviral propertiesantiviral propertiesSubject Category: Properties
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    , carbohydratescarbohydratesSubject Category: Chemicals and Chemical Groups
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    , cell linescell linesSubject Category: Organism Groups
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    , chemical compositionchemical compositionSubject Category: Properties
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    , dosage effectsdosage effectsSubject Category: Properties
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    , fatsfatsSubject Category: Chemicals and Chemical Groups
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    , flavonoidsflavonoidsSubject Category: Chemicals and Chemical Groups
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    , glycosidesglycosidesSubject Category: Chemicals and Chemical Groups
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    , in vitroin vitroSubject Category: Techniques, Methodologies and Equipment
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    , plant extractsplant extractsSubject Category: Commodities and Products
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    , poliomyelitispoliomyelitisSubject Category: Diseases, Disorders, and Symptoms
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    , protein contentprotein contentSubject Category: Properties
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    , reducing sugarsreducing sugarsSubject Category: Chemicals and Chemical Groups
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    , resinsresinsSubject Category: Chemicals and Chemical Groups
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    , saponinssaponinsSubject Category: Chemicals and Chemical Groups
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    , tanninstanninsSubject Category: Chemicals and Chemical Groups
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    , tissue culturetissue cultureSubject Category: Techniques, Methodologies and Equipment
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    Identifier(s) : anti-viral properties, heterosides, polio, saccharides, tannic acid

    Broader term(s) : GynostemmagynostemmaSubject Category: Organism Names
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    , CucurbitaceaecucurbitaceaeSubject Category: Organism Names
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    , CucurbitalescucurbitalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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