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  • Localization of pufferfish saxitoxin and tetrodotoxin binding protein (PSTBP) in the tissues of the pufferfish, Takifugu pardalis, analyzed by immunohistochemical staining.

    Author(s) : Yotsu-Yamashita, M.Okoshi, N.Watanabe, K.Araki, N.Yamaki, H.Shoji, Y.Terakawa, T.

    Author Affiliation : Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.

    Author Email : myama@biochem.tohoku.ac.jp

    Journal article : Toxicon 2013 Vol.72 pp.23-28 ref.30

    Abstract : Pufferfish saxitoxin and tetrodotoxintetrodotoxinSubject Category: Chemicals and Chemical Groups
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    binding proteinproteinSubject Category: Miscellaneous
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    (PSTBP) was previously isolated from the plasma of the marine pufferfish, Takifugu pardalis. In this study, we investigated distributiondistributionSubject Category: Properties
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    pattern of PSTBP in intestine, liver, ovary, skin, and skeletal muscle of T. pardalis by immunohistochemical stainingstainingSubject Category: Techniques, Methodologies and Equipment
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    for the study of functions of this protein. In the skin, dermis around the tetrodotoxin secreting gland was positive, while this secreting gland itself was negative. In the ovary containing vitellogenic oocytes, ovarian wall and vitelline envelope were positive, while yolk and nucleus were negative. In the liver, hepatocytes with large fat dropletsdropletsSubject Category: Miscellaneous
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    and capillaries were positive. In the intestine, the lamina propria mucosae were positive, while the mucosal epithelium was negative. In the skeletal muscle, only capillaries were positive. Furthermore, liver specific expression of PSTBP was confirmed by Northern blot analysisanalysisSubject Category: Techniques, Methodologies and Equipment
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    . Based on these results together with reported tetrodotoxin localizationlocalizationSubject Category: Properties
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    pattern in pufferfish, PSTBP was assumed to be a carrier protein to transfer tetrodotoxin among the tissuestissuesSubject Category: Anatomical and Morphological Structures
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    , especially liver, ovary, and skin.

    ISSN : 0041-0101

    DOI : 10.1016/j.toxicon.2013.06.002

    Record Number : 20133328882

    Publisher : Elsevier Ltd

    Location of publication : Oxford

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Descriptor(s) : analysisanalysisSubject Category: Techniques, Methodologies and Equipment
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    , bindingbindingSubject Category: Miscellaneous
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    , binding proteinsbinding proteinsSubject Category: Chemicals and Chemical Groups
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    , distributiondistributionSubject Category: Properties
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    , dropletsdropletsSubject Category: Miscellaneous
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    , intestinesintestinesSubject Category: Anatomical and Morphological Structures
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    , localizationlocalizationSubject Category: Properties
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    , musclesmusclesSubject Category: Anatomical and Morphological Structures
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    , nucleinucleiSubject Category: Anatomical and Morphological Structures
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    , ovariesovariesSubject Category: Anatomical and Morphological Structures
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    , proteinproteinSubject Category: Miscellaneous
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    , stainingstainingSubject Category: Techniques, Methodologies and Equipment
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    , tissuestissuesSubject Category: Anatomical and Morphological Structures
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    , tetrodotoxintetrodotoxinSubject Category: Chemicals and Chemical Groups
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    , toxinstoxinsSubject Category: Chemicals and Chemical Groups
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    Identifier(s) : carrier proteins, cell nuclei