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  • Replication characteristics of porcine reproductive and respiratory syndrome virus (PRRSV) European subtype 1 (Lelystad) and subtype 3 (Lena) strains in nasal mucosa and cells of the monocytic lineage: indications for the use of new receptors of PRRSV (Lena).

    Author(s) : Frydas, I. S.Verbeeck, M.Cao JunNauwynck, H. J.

    Author Affiliation : Laboratory of Virology, Department of Virology, Immunology and Parasitology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.

    Author Email : ilias.frydas@ugent.bemieke.verbeeck@ugent.beznps@163.comhans.nauwynck@ugent.be

    Journal article : Veterinary Research 2013 Vol.44 No.73 pp.(4 September 2013) ref.49

    Abstract : Recently, it has been demonstrated that subtype 3 strainsstrainsSubject Category: Organism Groups
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    of European type porcine reproductive and respiratory syndrome virusporcine reproductive and respiratory syndrome virusSubject Category: Organism Names
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    (PRRSV) are more virulent/pathogenic than subtype 1 strains. This points to differences in the pathogenesispathogenesisSubject Category: Miscellaneous
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    . In the present study, a new polarized nasal mucosamucosaSubject Category: Anatomical and Morphological Structures
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    explant system was used to study the invasion of the low virulent subtype 1 PRRSV strain Lelystad (LV) and the highly virulent subtype 3 PRRSV strain Lena at the portal of entry. Different cell types of the monocytic lineage (alveolar macrophagesmacrophagesSubject Category: Anatomical and Morphological Structures
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    (PAM), cultured blood monocytesmonocytesSubject Category: Anatomical and Morphological Structures
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    and monocyte-derived dendritic cellsdendritic cellsSubject Category: Anatomical and Morphological Structures
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    (moDC)) were enclosed to examine replication kineticskineticsSubject Category: Miscellaneous
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    of both strains in their putative target cells. At 0, 12, 24, 48 and 72 hours post inoculationinoculationSubject Category: Techniques, Methodologies and Equipment
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    (hpi), virus productionproductionSubject Category: Miscellaneous
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    was analyzed and the infected cells were quantified and identified. Lena replicated much more efficiently than LV in the nasal mucosa explants and to a lesser extent in PAM. Differences in replication were not found in monocytes and moDC. Confocal microscopy demonstrated that for LV, almost all viral antigen positive cells were CD163+Sialoadhesin (Sn)+, which were mainly located in the lamina propria of the respiratory mucosa. In Lena-infected nasal mucosa, CD163+Sn+, CD163+Sn- and to a lesser extent CD163-Sn- monocytic subtypes were involved in infection. CD163+Sn- cells were mostly located within or in the proximity of the epitheliumepitheliumSubject Category: Anatomical and Morphological Structures
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    . Our results show that, whereas LV replicates in a restricted subpopulation of CD163+Sn+ monocytic cells in the upper respiratory tract, Lena hijacks a broader range of subpopulations to spread within the mucosa. Replication in CD163+Sn- cells suggests that an alternative entry receptor may contribute to the wider tropism of Lena.

    ISSN : 1297-9716

    URL : http://www.veterinaryresearch.org/con...

    Record Number : 20133329625

    Publisher : BioMed Central Ltd

    Location of publication : London

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : ArterivirusarterivirusSubject Category: Organism Names
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    , pigspigsSubject Category: Organism Names
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    , Porcine reproductive and respiratory syndrome virusporcine reproductive and respiratory syndrome virusSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    Descriptor(s) : antigensantigensSubject Category: Chemicals and Chemical Groups
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    , characterizationcharacterizationSubject Category: Miscellaneous
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    , dendritic cellsdendritic cellsSubject Category: Anatomical and Morphological Structures
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    , epitheliumepitheliumSubject Category: Anatomical and Morphological Structures
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    , immunizationimmunizationSubject Category: Techniques, Methodologies and Equipment
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    , inoculationinoculationSubject Category: Techniques, Methodologies and Equipment
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    , kineticskineticsSubject Category: Miscellaneous
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    , macrophagesmacrophagesSubject Category: Anatomical and Morphological Structures
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    , monocytesmonocytesSubject Category: Anatomical and Morphological Structures
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    , mucosamucosaSubject Category: Anatomical and Morphological Structures
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    , nosenoseSubject Category: Anatomical and Morphological Structures
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    , pathogenesispathogenesisSubject Category: Miscellaneous
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    , productionproductionSubject Category: Miscellaneous
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    , receptorsreceptorsSubject Category: Anatomical and Morphological Structures
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    , respiratory systemrespiratory systemSubject Category: Anatomical and Morphological Structures
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    , strainsstrainsSubject Category: Organism Groups
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    , viral diseasesviral diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , virulencevirulenceSubject Category: Miscellaneous
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    Identifier(s) : alveolar macrophages, antigenicity, hogs, immune sensitization, immunogens, mucous membrane, subtypes, swine, syndromes, viral infections

    Broader term(s) : ArteriviridaearteriviridaeSubject Category: Organism Names
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    , NidoviralesnidoviralesSubject Category: Organism Names
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    , positive-sense ssRNA Virusespositive-sense ssrna virusesSubject Category: Organism Names
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    , ssRNA Virusesssrna virusesSubject Category: Organism Names
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    , RNA Virusesrna virusesSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    , Sus scrofasus scrofaSubject Category: Organism Names
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    , SussusSubject Category: Organism Names
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    , SuidaesuidaeSubject Category: Organism Names
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    , SuiformessuiformesSubject Category: Organism Names
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    , ArtiodactylaartiodactylaSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , ArterivirusarterivirusSubject Category: Organism Names
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