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  • Production and characterization of novel fibrinolytic enzyme from different soil fungal sp.

    Author(s) : Rashmi, B. S.Liny, P.

    Author Affiliation : Department of Biotechnology, R.V. College of Engineering, Bangalore - 560 059, Karnataka, India.

    Author Email : rashmibs.bs@gmail.com

    Journal article : International Journal of Pharma and Bio Sciences 2013 Vol.4 No.3 pp.B-454-B-463 ref.25

    Abstract : Different fungal species, Aspergillus nigeraspergillus nigerSubject Category: Organism Names
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    , Aspergillus flavusaspergillus flavusSubject Category: Organism Names
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    and Pencillium notatum selected for the production of extracellular fibrinolytic enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , Production rate was enhanced by using different carbon source where maltosemaltoseSubject Category: Chemicals and Chemical Groups
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    increased the production rate of enzymes 2.4 mg/ml in Aspergillus niger, 2.2 mg/ml Aspergillus flavus and glucoseglucoseSubject Category: Chemicals and Chemical Groups
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    2.2 mg/ml in Pencillium notatum. Enzyme was fractionated using ammonium sulfate fractionation, different characteristic studies like protease activity showed 1.4 units/mg of proteins in Aspergillus niger, 1.0 units/mg of proteins in Aspergillus flavus, 1.3 units/mg of proteins in Penicillium notatum. Fibrinplate method showed 200 µg/ml, 600 µg/ml, 240 µg/ml in Aspergillus niger, Aspergillus flavus, Penicillium notatum respectively and anticoagulation clottingclottingSubject Category: Miscellaneous
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    time assay showed 25, 20, 15 min delay in clotting activity in Aspergillus niger, Penicillium notatum, Aspergillus flavus respectively. The Protein bands found on SDS-PAGE for fibrinolytic enzymes from different fungal sp were approximately 14 kD from Penicillium notatum, 27 kD from Aspergillus niger, 26 kD from Aspergillus flavus. The current research showed fibrinolytic enzyme enhancement in maltose and glucose and its characterization specified that be a novel method for yield enhancement.

    ISSN : 0975-6299

    URL : http://www.ijpbs.net/.../2557_pdf.pdf

    Record Number : 20133392358

    Publisher : International Journal of Pharma and Bio Sciences

    Location of publication : Andrapradesh

    Country of publication : India

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Aspergillus flavusaspergillus flavusSubject Category: Organism Names
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    , Aspergillus nigeraspergillus nigerSubject Category: Organism Names
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    , Penicillium chrysogenumpenicillium chrysogenumSubject Category: Organism Names
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    Descriptor(s) : clottingclottingSubject Category: Miscellaneous
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    , enzyme activityenzyme activitySubject Category: Properties
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , glucoseglucoseSubject Category: Chemicals and Chemical Groups
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    , maltosemaltoseSubject Category: Chemicals and Chemical Groups
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    , proteinasesproteinasesSubject Category: Chemicals and Chemical Groups
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    , soil florasoil floraSubject Category: Organism Groups
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    , soil fungisoil fungiSubject Category: Organism Groups
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    Identifier(s) : dextrose, fungus, proteases

    Broader term(s) : AspergillusaspergillusSubject Category: Organism Names
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    , TrichocomaceaetrichocomaceaeSubject Category: Organism Names
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    , EurotialeseurotialesSubject Category: Organism Names
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    , EurotiomyceteseurotiomycetesSubject Category: Organism Names
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    , PezizomycotinapezizomycotinaSubject Category: Organism Names
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    , AscomycotaascomycotaSubject Category: Organism Names
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    , fungifungiSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , PenicilliumpenicilliumSubject Category: Organism Names
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