You are signed in as:
  • IFPRI
  • Signed in via:
  • IP Address

  • Choose a colour
  • Cloning, functional characterization, and expression profiles of NADPH-cytochrome P450 reductase gene from the Asiatic rice striped stem borer, Chilo suppressalis (Lepidoptera: Pyralidae).

    Author(s) : Liu SuLiang QingMeiHuang YuanJieYuan XinZhou WenWuQiao FeiCheng JiaAnGurr, G. M.Zhu ZengRong

    Author Affiliation : State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.

    Author Email : zrzhu@zju.edu.cn

    Journal article : Comparative Biochemistry and Physiology. B, Biochemistry & Molecular Biology 2013 Vol.166 No.3/4 pp.225-231

    Abstract : NADPH-cytochrome P450 reductase (CPR) is one of the most important components of the cytochrome P450 enzyme system. It catalyzes electron transferelectron transferSubject Category: Miscellaneous
    see more details
    from NADPH to all known P450s, thus plays central roles not only in the metabolismmetabolismSubject Category: Natural Processes
    see more details
    of exogenous xenobioticsxenobioticsSubject Category: Chemicals and Chemical Groups
    see more details
    but also in the regulation of endogenous hormoneshormonesSubject Category: Chemicals and Chemical Groups
    see more details
    in insectsinsectsSubject Category: Organism Names
    see more details
    . In this study, a full-length cDNA encoding of a CPR (named CsCPR) was isolated from the Asiatic rice striped stem borer, Chilo suppressalischilo suppressalisSubject Category: Organism Names
    see more details
    , by using reverse transcription-polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
    see more details
    (RT-PCR) and rapid amplificationamplificationSubject Category: Techniques, Methodologies and Equipment
    see more details
    of cDNA ends (RACE) methods. The cDNA contains a 2061 bp open reading frame, which encodes an enzyme of 686 amino acid residuesresiduesSubject Category: Chemicals and Chemical Groups
    see more details
    , with a calculated molecular mass of 77.6 kDa. The deduced peptide has hallmarks of typical CPR, including an N-terminal membrane anchor and the FMN, FAD and NADPH binding domains. The N-terminal-truncated protein fused with a 6 × His.tag was heterologously expressed in Escherichia coliescherichia coliSubject Category: Organism Names
    see more details
    Rosetta (DE3) cells and purified, specific activity and the Km values of the recombinant enzyme were determined. Tissue- and developmental stage-dependent expression of CsCPR mRNA was investigated by real-time quantitative PCR. The CsCPR mRNA was noticeably expressed in the digestive, metabolic, and olfactory organs of the larvae and adults of C. suppressalis. Our initial results would provide valuable information for further study on the interactionsinteractionsSubject Category: Miscellaneous
    see more details
    between CPR and cytochrome P450 enzyme systems.

    ISSN : 1096-4959

    DOI : 10.1016/j.cbpb.2013.09.004

    Record Number : 20133392889

    Publisher : Elsevier Ltd

    Location of publication : Oxford

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : ChilochiloSubject Category: Organism Names
    see more details
    , Chilo suppressalischilo suppressalisSubject Category: Organism Names
    see more details
    , EscherichiaescherichiaSubject Category: Organism Names
    see more details
    , Escherichia coliescherichia coliSubject Category: Organism Names
    see more details
    , insectsinsectsSubject Category: Organism Names
    see more details
    , LepidopteralepidopteraSubject Category: Organism Names
    see more details
    , PyralidaepyralidaeSubject Category: Organism Names
    see more details

    Descriptor(s) : amplificationamplificationSubject Category: Techniques, Methodologies and Equipment
    see more details
    , biochemistrybiochemistrySubject Category: Disciplines, Occupations and Industries
    see more details
    , characterizationcharacterizationSubject Category: Miscellaneous
    see more details
    , clonesclonesSubject Category: Miscellaneous
    see more details
    , complementary DNAcomplementary dnaSubject Category: Chemicals and Chemical Groups
    see more details
    , cytochrome ccytochrome cSubject Category: Chemicals and Chemical Groups
    see more details
    , cytochrome P-450cytochrome p-450Subject Category: Chemicals and Chemical Groups
    see more details
    , electron transferelectron transferSubject Category: Miscellaneous
    see more details
    , gene expressiongene expressionSubject Category: Miscellaneous
    see more details
    , genesgenesSubject Category: Miscellaneous
    see more details
    , hormoneshormonesSubject Category: Chemicals and Chemical Groups
    see more details
    , interactionsinteractionsSubject Category: Miscellaneous
    see more details
    , messenger RNAmessenger rnaSubject Category: Chemicals and Chemical Groups
    see more details
    , metabolismmetabolismSubject Category: Natural Processes
    see more details
    , molecular biologymolecular biologySubject Category: Disciplines, Occupations and Industries
    see more details
    , nucleotide sequencesnucleotide sequencesSubject Category: Chemicals and Chemical Groups
    see more details
    , open reading framesopen reading framesSubject Category: Chemicals and Chemical Groups
    see more details
    , polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
    see more details
    , residuesresiduesSubject Category: Chemicals and Chemical Groups
    see more details
    , stem borersstem borersSubject Category: Organism Groups
    see more details
    , xenobioticsxenobioticsSubject Category: Chemicals and Chemical Groups
    see more details

    Identifier(s) : bacterium, cDNA, cloning, DNA sequences, E. coli, electron flow, electron transport, mRNA, ORFs, PCR, stem-borers, stemborers

    Broader term(s) : insectsinsectsSubject Category: Organism Names
    see more details
    , HexapodahexapodaSubject Category: Organism Names
    see more details
    , arthropodsarthropodsSubject Category: Organism Names
    see more details
    , invertebratesinvertebratesSubject Category: Organism Names
    see more details
    , animalsanimalsSubject Category: Organism Names
    see more details
    , eukaryoteseukaryotesSubject Category: Organism Names
    see more details
    , PyralidaepyralidaeSubject Category: Organism Names
    see more details
    , LepidopteralepidopteraSubject Category: Organism Names
    see more details
    , ChilochiloSubject Category: Organism Names
    see more details
    , EnterobacteriaceaeenterobacteriaceaeSubject Category: Organism Names
    see more details
    , EnterobacterialesenterobacterialesSubject Category: Organism Names
    see more details
    , GammaproteobacteriagammaproteobacteriaSubject Category: Organism Names
    see more details
    , ProteobacteriaproteobacteriaSubject Category: Organism Names
    see more details
    , BacteriabacteriaSubject Category: Organism Names
    see more details
    , prokaryotesprokaryotesSubject Category: Organism Names
    see more details
    , EscherichiaescherichiaSubject Category: Organism Names
    see more details