Author(s) :
Liu Su
;
Liang QingMei
;
Huang YuanJie
;
Yuan Xin
;
Zhou WenWu
;
Qiao Fei
;
Cheng JiaAn
;
Gurr, G. M.
;
Zhu ZengRong
Author Affiliation :
State Key Laboratory of Rice Biology, Key Laboratory of Agricultural Entomology, Ministry of Agriculture, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Author Email :
zrzhu@zju.edu.cn
Journal article
:
Comparative Biochemistry and Physiology. B, Biochemistry & Molecular Biology
2013
Vol.166
No.3/4
pp.225-231
Abstract :
NADPH-cytochrome P450 reductase (CPR) is one of the most important components of the cytochrome P450 enzyme system. It catalyzes
electron transferelectron transferSubject Category: Miscellaneous
see more details from NADPH to all known P450s, thus plays central roles not only in the
metabolismmetabolismSubject Category: Natural Processes
see more details of exogenous
xenobioticsxenobioticsSubject Category: Chemicals and Chemical Groups
see more details but also in the regulation of endogenous
hormoneshormonesSubject Category: Chemicals and Chemical Groups
see more details in
insectsinsectsSubject Category: Organism Names
see more details. In this study, a full-length cDNA encoding of a CPR (named CsCPR) was isolated from the Asiatic rice striped stem borer,
Chilo suppressalischilo suppressalisSubject Category: Organism Names
see more details, by using reverse transcription-
polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
see more details (RT-PCR) and rapid
amplificationamplificationSubject Category: Techniques, Methodologies and Equipment
see more details of cDNA ends (RACE) methods. The cDNA contains a 2061 bp open reading frame, which encodes an enzyme of 686 amino acid
residuesresiduesSubject Category: Chemicals and Chemical Groups
see more details, with a calculated molecular mass of 77.6 kDa. The deduced peptide has hallmarks of typical CPR, including an N-terminal membrane anchor and the FMN, FAD and NADPH binding domains. The N-terminal-truncated protein fused with a 6 × His.tag was heterologously expressed in
Escherichia coliescherichia coliSubject Category: Organism Names
see more details Rosetta (DE3) cells and purified, specific activity and the
Km values of the recombinant enzyme were determined. Tissue- and developmental stage-dependent expression of
CsCPR mRNA was investigated by real-time quantitative PCR. The
CsCPR mRNA was noticeably expressed in the digestive, metabolic, and olfactory organs of the larvae and adults of
C. suppressalis. Our initial results would provide valuable information for further study on the
interactionsinteractionsSubject Category: Miscellaneous
see more details between CPR and cytochrome P450 enzyme systems.
ISSN :
1096-4959
DOI :
10.1016/j.cbpb.2013.09.004
Record Number :
20133392889
Publisher :
Elsevier Ltd
Location of publication :
Oxford
Country of publication :
UK
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
ChilochiloSubject Category: Organism Names
see more details, Chilo suppressalischilo suppressalisSubject Category: Organism Names
see more details, EscherichiaescherichiaSubject Category: Organism Names
see more details, Escherichia coliescherichia coliSubject Category: Organism Names
see more details, insectsinsectsSubject Category: Organism Names
see more details, LepidopteralepidopteraSubject Category: Organism Names
see more details, PyralidaepyralidaeSubject Category: Organism Names
see more details
Descriptor(s) :
amplificationamplificationSubject Category: Techniques, Methodologies and Equipment
see more details, biochemistrybiochemistrySubject Category: Disciplines, Occupations and Industries
see more details, characterizationcharacterizationSubject Category: Miscellaneous
see more details, clonesclonesSubject Category: Miscellaneous
see more details, complementary DNAcomplementary dnaSubject Category: Chemicals and Chemical Groups
see more details, cytochrome ccytochrome cSubject Category: Chemicals and Chemical Groups
see more details, cytochrome P-450cytochrome p-450Subject Category: Chemicals and Chemical Groups
see more details, electron transferelectron transferSubject Category: Miscellaneous
see more details, gene expressiongene expressionSubject Category: Miscellaneous
see more details, genesgenesSubject Category: Miscellaneous
see more details, hormoneshormonesSubject Category: Chemicals and Chemical Groups
see more details, interactionsinteractionsSubject Category: Miscellaneous
see more details, messenger RNAmessenger rnaSubject Category: Chemicals and Chemical Groups
see more details, metabolismmetabolismSubject Category: Natural Processes
see more details, molecular biologymolecular biologySubject Category: Disciplines, Occupations and Industries
see more details, nucleotide sequencesnucleotide sequencesSubject Category: Chemicals and Chemical Groups
see more details, open reading framesopen reading framesSubject Category: Chemicals and Chemical Groups
see more details, polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
see more details, residuesresiduesSubject Category: Chemicals and Chemical Groups
see more details, stem borersstem borersSubject Category: Organism Groups
see more details, xenobioticsxenobioticsSubject Category: Chemicals and Chemical Groups
see more details
Identifier(s) :
bacterium, cDNA, cloning, DNA sequences, E. coli, electron flow, electron transport, mRNA, ORFs, PCR, stem-borers, stemborers
Broader term(s) :
insectsinsectsSubject Category: Organism Names
see more details, HexapodahexapodaSubject Category: Organism Names
see more details, arthropodsarthropodsSubject Category: Organism Names
see more details, invertebratesinvertebratesSubject Category: Organism Names
see more details, animalsanimalsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details, PyralidaepyralidaeSubject Category: Organism Names
see more details, LepidopteralepidopteraSubject Category: Organism Names
see more details, ChilochiloSubject Category: Organism Names
see more details, EnterobacteriaceaeenterobacteriaceaeSubject Category: Organism Names
see more details, EnterobacterialesenterobacterialesSubject Category: Organism Names
see more details, GammaproteobacteriagammaproteobacteriaSubject Category: Organism Names
see more details, ProteobacteriaproteobacteriaSubject Category: Organism Names
see more details, BacteriabacteriaSubject Category: Organism Names
see more details, prokaryotesprokaryotesSubject Category: Organism Names
see more details, EscherichiaescherichiaSubject Category: Organism Names
see more details