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  • Minimal lethal concentration of hyrgromycin B in calli induction and shoot multiplication process of Digitalis purpurea L.

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    Foreign Title : Concentración mínima letal de higromicina B en la formación de callos y multiplicación de brotes de Digitalis purpurea L.

    Author(s) : Hernández-Díaz, E. K.Pérez-Alonso, N.Capote, A.Pérez, A.Jiménez, E.Chong-Pérez, B.

    Author Affiliation : Departamento de Biología, Facultad de Ciencias Agropecuarias, Universidad Central Marta Abreu de Las Villas, Carretera a Camajuaní km 5.5. Santa Clara, Villa Clara C.P. 54 830, Cuba.

    Author Email : boris@ibp.co.cu

    Journal article : Biotecnología Vegetal 2013 Vol.13 No.1 pp.23-31 ref.18

    Abstract : The plantsplantsSubject Category: Organism Names
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    of the genus Digitalis are characterized by the production of cardenolides, drugs widely used worldwide in the treatment of heart failure. In previous research a transformation protocol was developed from leaf disc of Digitalis purpureadigitalis purpureaSubject Category: Organism Names
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    L., using geneticin as selection marker. However some escapes in the selection process were obtained. So it is necessary to develop a more efficient selection scheme using another selective agent. Therefore, the aim of the present research was to select the minimum lethal concentration of hygromycin B during calluscallusSubject Category: Anatomical and Morphological Structures
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    induction and shootsshootsSubject Category: Anatomical and Morphological Structures
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    multiplication of D. purpurea. For callus induction we studied five concentrations of hygromycine B (3, 6, 9, 12, 15 mg l-1) during 28 days. Besides, the effect in shoot multiplication of four concentrations of hygromycine B (25, 50, 75, 100 mg l-1) was studied during 30 days. The minimal lethal concentration for callus formation was 12 mg l-1. In the case of shoot multiplication, 100% mortality was showed at 75 mg l-1 strictly after 30 days. The proposed selection scheme is recommended for future work at genetic transformation in this species.

    ISSN : 1609-1841

    URL : http://revista.ibp.co.cu/component/do...

    Record Number : 20143334565

    Publisher : Instituto de Biotecnología de las Plantas

    Location of publication : Villa Clara

    Country of publication : Cuba

    Language of text : Spanish

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Digitalis purpureadigitalis purpureaSubject Category: Organism Names
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    Descriptor(s) : calluscallusSubject Category: Anatomical and Morphological Structures
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    , leavesleavesSubject Category: Anatomical and Morphological Structures
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    , shootsshootsSubject Category: Anatomical and Morphological Structures
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    , aminoglycoside antibioticsaminoglycoside antibioticsSubject Category: Chemicals and Chemical Groups
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    , antibioticsantibioticsSubject Category: Chemicals and Chemical Groups
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    Identifier(s) : hyrgromycin

    Broader term(s) : DigitalisdigitalisSubject Category: Organism Names
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    , ScrophulariaceaescrophulariaceaeSubject Category: Organism Names
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    , LamialeslamialesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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