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  • Isolation and expression analysis of cinnamic acid 4-hydroxylase gene under different temperatures in Cryptotaenia japonica Hassk.

    Author(s) : Tan GuoFeiWang FengWang GuangLongXu ZhiShengXiong AiSheng

    Author Affiliation : State Key Laboratory of Crop Genetics and Germplasm Enhancement, Ministry of Agriculture Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.

    Author Email : 2012104080@njau.edu.cnXiongaisheng@njau.edu.cn

    Journal article : Acta Botanica Boreali-Occidentalia Sinica 2014 Vol.34 No.7 pp.1298-1301 ref.29

    Abstract : In order to study the lignin synthesis related genesgenesSubject Category: Miscellaneous
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    of Cryptotaenia japonicacryptotaenia japonicaSubject Category: Organism Names
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    Hassk, the gene CjCAH with 1 631 bp, which encoding the cinnamate 4-hydroxylase (C4H) involved into lignin synthesis, was cloned from wild variety C. japonica Hassk of GuizhouguizhouSubject Category: Geographic Entities
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    Province. C. japonica Hassk was grown under 4 different temperatures (10°C, 18°C, 30°C and 38°C). The CjC4H genes expression was detected at 0, 0.5, 1, 2, 4, 8, 12 and 24 h to provide reference for C. japonica Hassk suitable growth temperaturetemperatureSubject Category: Properties
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    selection. The ORF (Open Reading Frame) length was 1 521 bp, which encoding 506 amino acids. The molecular weight of the enzyme of CjC4H was 58.13 kD, and the pI was 9.38. Phylogenetie analysis demonstrated that the enzyme of C4H relative conservative in different plant species. The CjC4H genes expression under different temperature detected by real time quantitative PCR, the results showed that the CjC4H gene expressiongene expressionSubject Category: Miscellaneous
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    under high temperature of 38°C 4 h was much higher than that of other temperature treatments, and the expression of CjC4H increased significantly at 24 h under 10°C and 18°C low temperature treatments.

    ISSN : 1000-4025

    URL : http://xbzwxb.nwsuaf.edu.cn

    Record Number : 20143342256

    Publisher : Science Press

    Location of publication : Beijing

    Country of publication : China

    Language of text : Chinese

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Cryptotaenia japonicacryptotaenia japonicaSubject Category: Organism Names
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    Descriptor(s) : DNA cloningdna cloningSubject Category: Techniques, Methodologies and Equipment
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    , gene expressiongene expressionSubject Category: Miscellaneous
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    , genesgenesSubject Category: Miscellaneous
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    , heat stressheat stressSubject Category: Miscellaneous
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    , open reading framesopen reading framesSubject Category: Chemicals and Chemical Groups
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    , phylogeneticsphylogeneticsSubject Category: Disciplines, Occupations and Industries
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    , real time PCRreal time pcrSubject Category: Techniques, Methodologies and Equipment
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    , temperaturetemperatureSubject Category: Properties
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    Identifier(s) : Araliales, Kweichow, ORFs, People's Republic of China, trans-cinnamate 4-monooxygenase

    Geographical Location(s) : ChinachinaSubject Category: Geographic Entities
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    , GuizhouguizhouSubject Category: Geographic Entities
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    Broader term(s) : CryptotaeniacryptotaeniaSubject Category: Organism Names
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    , ApiaceaeapiaceaeSubject Category: Organism Names
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    , ApialesapialesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , APEC countriesapec countriesSubject Category: Institutions and Organisations
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    , Developing Countriesdeveloping countriesSubject Category: Miscellaneous
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    , East Asiaeast asiaSubject Category: Geographic Entities
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    , AsiaasiaSubject Category: Geographic Entities
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    , South Western Chinasouth western chinaSubject Category: Geographic Entities
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    , ChinachinaSubject Category: Geographic Entities
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