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  • Pathogenesis of porcine epidemic diarrhea virus isolate (US/Iowa/18984/2013) in 3-week-old weaned pigs.

    Author(s) : Madson, D. M.Magstadt, D. R.Arruda, P. H. E.Hoang, H.Sun, D.Bower, L. P.Bhandari, M.Burrough, E. R.Gauger, P. C.Pillatzki, A. E.Stevenson, G. W.Wilberts, B. L.Brodie, J.Harmon, K. M.Wang, C.Main, R. G.Zhang, J.Yoon, K. J.

    Author Affiliation : Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA 50011, USA.

    Author Email : madson@iastate.edukyoon@iastate.edu

    Journal article : Veterinary Microbiology 2014 Vol.174 No.1/2 pp.60-68 ref.23

    Abstract : Porcine epidemic diarrhea virusporcine epidemic diarrhea virusSubject Category: Organism Names
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    (PEDV) is associated with clinical diarrhea in naïve swine of all ages. This report describes timingtimingSubject Category: Miscellaneous
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    of antibody generation and disease progression following infection with a US PEDV isolate by assessing fecal viral sheddingsheddingSubject Category: Miscellaneous
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    , morphometric analysisanalysisSubject Category: Techniques, Methodologies and Equipment
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    of intestinal lesionslesionsSubject Category: Diseases, Disorders, and Symptoms
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    , and magnitude of immunohistochemical stainingstainingSubject Category: Techniques, Methodologies and Equipment
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    . Sixty-three, 3-week-old pigspigsSubject Category: Organism Names
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    were randomly allocated into control (n=27) and challenged (n=36) groups. Challenged pigs were administered 1 mL of 1×103 PFU/mL of US/IowaiowaSubject Category: Geographic Entities
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    /18984/2013 PEDV isolate by oro-gastric gavage. Three control and four challenged pigs were necropsied on days post-inoculation (dpi) 1, 2, 3, 4, 7, and weekly thereafter, until study termination on dpi 35. Clinical disease, fecal shedding, body weightbody weightSubject Category: Properties
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    , and temperaturetemperatureSubject Category: Properties
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    were monitored during the study period. Diarrhea was observed in challenged pigs beginning for some on dpi 2, affecting a majority of pigs by dpi 6 and subsiding by dpi 10. Average daily gain was significantly lower (P<0.001) for one week post-infection in challenged pigs. PEDV was detected in fecesfecesSubject Category: Miscellaneous
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    fecesSubject Category: Miscellaneous
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    by PCR on dpi 1 and continued in a subset of pigs until dpi 24. PEDV-specific antigen was detected in villous enterocytes of challenged pigs by immunohistochemistryimmunohistochemistrySubject Category: Disciplines, Occupations and Industries
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    (IHC) on dpi 1, 2, 3, 4, 7, and 14. Microscopic lesions included severe diffuse atrophic enteritisenteritisSubject Category: Diseases, Disorders, and SymptomssymptomsSubject Category: Diseases, Disorders, and Symptoms
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    with significantly reduced (P<0.001) villous length observed on dpi 3, 4, and 7. Under the conditions of this study, fecal shedding of PEDV and IHC staining can precede and continue beyond the observation of clinical signs, thus increasing the risk of viral transmissiontransmissionSubject Category: Miscellaneous
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    .

    ISSN : 0378-1135

    DOI : 10.1016/j.vetmic.2014.09.002

    URL : http://www.sciencedirect.com/science/...

    Record Number : 20143421779

    Publisher : Elsevier B. V.

    Location of publication : Amsterdam

    Country of publication : Netherlands

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : pigspigsSubject Category: Organism Names
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    , Porcine epidemic diarrhea virusporcine epidemic diarrhea virusSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    Descriptor(s) : analysisanalysisSubject Category: Techniques, Methodologies and Equipment
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    , antibodiesantibodiesSubject Category: Chemicals and Chemical Groups
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    , antigensantigensSubject Category: Chemicals and Chemical Groups
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    , body weightbody weightSubject Category: Properties
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    , clinical aspectsclinical aspectsSubject Category: Miscellaneous
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    , diarrhoeadiarrhoeaSubject Category: Diseases, Disorders, and Symptoms
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    , disease coursedisease courseSubject Category: Diseases, Disorders, and Symptoms
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    , enteritisenteritisSubject Category: Diseases, Disorders, and Symptoms
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    , epidemicsepidemicsSubject Category: Miscellaneous
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    , faecesfaecesSubject Category: Miscellaneous
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    , immunohistochemistryimmunohistochemistrySubject Category: Disciplines, Occupations and Industries
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    , infectionsinfectionsSubject Category: Diseases, Disorders, and Symptoms
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    , intestinesintestinesSubject Category: Anatomical and Morphological Structures
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    , lesionslesionsSubject Category: Diseases, Disorders, and Symptoms
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    , liveweight gainliveweight gainSubject Category: Properties
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    , pathogenesispathogenesisSubject Category: Miscellaneous
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    , performance traitsperformance traitsSubject Category: Properties
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    , polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
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    , sheddingsheddingSubject Category: Miscellaneous
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    , stainingstainingSubject Category: Techniques, Methodologies and Equipment
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    , symptomssymptomsSubject Category: Diseases, Disorders, and Symptoms
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    , temperaturetemperatureSubject Category: Properties
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    , timingtimingSubject Category: Miscellaneous
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    , transmissiontransmissionSubject Category: Miscellaneous
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    , viral diseasesviral diseasesSubject Category: Diseases, Disorders, and Symptoms
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    Identifier(s) : antigenicity, clinical picture, diarrheadiarrheaSubject Category: Diseases, Disorders, and Symptoms
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    , disease progression, fecesfecesSubject Category: Miscellaneous
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    , hogs, ileitis, immunogens, isolates, jejunitis, liveweight gains, PCR, Porcine epidemic diarrhoea virus, scouring, swine, United States of America, viral infections

    Geographical Location(s) : IowaiowaSubject Category: Geographic Entities
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    , USAusaSubject Category: Geographic Entities
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    Broader term(s) : Sus scrofasus scrofaSubject Category: Organism Names
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    , SussusSubject Category: Organism Names
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    , SuidaesuidaeSubject Category: Organism Names
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    , SuiformessuiformesSubject Category: Organism Names
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    , ArtiodactylaartiodactylaSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , AlphacoronavirusalphacoronavirusSubject Category: Organism Names
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    , CoronavirinaecoronavirinaeSubject Category: Organism Names
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    , CoronaviridaecoronaviridaeSubject Category: Organism Names
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    , NidoviralesnidoviralesSubject Category: Organism Names
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    , positive-sense ssRNA Virusespositive-sense ssrna virusesSubject Category: Organism Names
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    , ssRNA Virusesssrna virusesSubject Category: Organism Names
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    , RNA Virusesrna virusesSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    , Corn Belt States of USAcorn belt states of usaSubject Category: Geographic Entities
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    , North Central States of USAnorth central states of usaSubject Category: Geographic Entities
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    , USAusaSubject Category: Geographic Entities
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    , APEC countriesapec countriesSubject Category: Institutions and Organisations
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    , Developed Countriesdeveloped countriesSubject Category: Miscellaneous
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    , North Americanorth americaSubject Category: Geographic Entities
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    , AmericaamericaSubject Category: Geographic Entities
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    , OECD Countriesoecd countriesSubject Category: Institutions and Organisations
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    , West North Central States of USAwest north central states of usaSubject Category: Geographic Entities
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