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  • Effect of methadone and valproate combination on morphine withdrawal-induced anxiety and depression in male mice.

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    Author(s) : Vahidi, S.Khalili, M.Kiasalari, Z.Yaghoutpoor, E.

    Author Affiliation : Department of Biology, Faculty of Sciences, Shahed University, Tehran, Iran.

    Author Email : samiravahidi@yahoo.com

    Journal article : Journal of Gorgan University of Medical Sciences 2015 Vol.16 No.4 pp.Pe21-Pe26, En27 ref.26

    Abstract : Background and Objective: Anxiety and depression are experienced following addicted patients drug withdrawal. This study was done to determine the effect of methadone and valproate combination on morphine withdrawal-induced anxiety and depression in male mice. Methods: In this experimental study, ninety-eight male mice were allocated into acute and chronic categories. Animals in acute chronic categories allocated into seven groups including: saline, morphine, methadone (10 mg/kg/bw), valproate (150 mg/kg/bw), three groups of valproate+methadone, in of ratio 1:1, 2:1 and 1:2. Animals were received escalating dose of morphine for 8 consecutive days except saline group. In chronic group, drugs were injected for 30 minutes before morphine administration, while in acute group; the drugs were used only at day 8. Anxiety and depression due to naloxone injection (5 mg/kg/bw) was investigated by elevated plus-maze, tail-suspension and open field tests. Results: In the chronic group, valproate + methadone (2:1) combination therapy showed a significant increase in the percentage of open arm entries (53.86±1.9) and percentage of time spent in the open arm (58.58±4.15) compared to the morphine group, with a percentage of entering (28.12±2.03) and percentage of time (17.88±1.77) (P<0.05). In open field test, the ratio of the number to the duration of time spent in the central square, in the combination therapy groups of methadone+valproate (27±2), valproate+methadone (1:2) and valproate+methadone (2:1) were significantly increased in compare to the morphine group (P<0.05). In tail-suspension test, duration of immobility as an indicator of depression, in the treatment group of valproate+methadone (2:1) was significantly reduced (P<0.05). Conclusion: Valproate and methadone combination therapy particularly in ratio of 2:1 can reduce morphine withdrawal-induced anxiety and depression in animal model.

    ISSN : 1562-4765

    URL : http://www.goums.ac.ir/journal

    Record Number : 20153041865

    Publisher : Golestan University of Medical Sciences and Health Services

    Location of publication : Gorgan

    Country of publication : Iran

    Language of text : Persian

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : mice

    Descriptor(s) : animal models, anxiety, depression, disease models, laboratory animals, mental disorders, mental stress, methadone, morphine, valproic acid

    Identifier(s) : mental illness, psychological stress

    Broader term(s) : Muridae, rodents, mammals, vertebrates, Chordata, animals, eukaryotes