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  • Effect of inoculum density and gaseous atmosphere renovation in Digitalis purpurea L. shoots culture in Temporary Immersion Systems.

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    Foreign Title : Efecto de la densidad de inóculo y la renovación de la atmósfera gaseosa en el cultivo de brotes de Digitalis purpurea L. en Sistemas de Inmersión Temporal.

    Author(s) : Pérez-Alonso, N.Capote, A.Pérez, A.Gerth, A.Chong-Pérez, B.Jiménez, E.

    Author Affiliation : Instituto de Biotecnología de las Plantas, Universidad Central Marta Abreu de Las Villas, Carretera a Camajuaní km 5.5, Santa Clara, Villa Clara, Cuba.

    Journal article : Biotecnología Vegetal 2015 Vol.15 No.1 pp.35-45 ref.38

    Abstract : Digitalis purpureadigitalis purpureaSubject Category: Organism Names
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    L. is one of the two unique species of economic interest in the genus Digitalis. It is important because is one of the principal source of cardenolides, irreplaceable drugs in the treatment of heart failure. The present work was done with the objective to determinate the effect of inoculum densityinoculum densitySubject Category: Properties
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    and gaseous atmosphereatmosphereSubject Category: Miscellaneous
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    renovation in D. purpurea plants cultivated in Temporal Immersion Systems (TIS). To do this, several parameters like inoculums density (6, 12, 18 explantsexplantsSubject Category: Miscellaneous
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    per TIS) and the use forced ventilationventilationSubject Category: Techniques, Methodologies and Equipment
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    were studied. The highest biomass productionbiomass productionSubject Category: Miscellaneous
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    was obtained when 12 explants were inoculated (104.03 gFW and 5.74 gDW). On the contrary, the lower inoculum density caused a negative effect in plant developmentplant developmentSubject Category: Natural Processes
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    and the biomass did not increase. On the other hand, the renovation of atmosphere mediated by forced ventilation induced a higher oxidative stressoxidative stressSubject Category: Miscellaneous
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    associated to an increase of digitoxindigitoxinSubject Category: Chemicals and Chemical Groups
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    synthesis. Both factors are of great importance for biomass and secondary metabolitessecondary metabolitesSubject Category: Chemicals and Chemical Groups
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    production in plants with pharmaceutical interest.

    ISSN : 1609-1841

    URL : http://revista.ibp.co.cu/2015/vol-15-...

    Record Number : 20153399289

    Publisher : Instituto de Biotecnología de las Plantas

    Location of publication : Villa Clara

    Country of publication : Cuba

    Language of text : Spanish

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Digitalis purpureadigitalis purpureaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    Descriptor(s) : atmosphereatmosphereSubject Category: Miscellaneous
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    , biomass productionbiomass productionSubject Category: Miscellaneous
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    , biosynthesisbiosynthesisSubject Category: Natural Processes
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    , digitoxindigitoxinSubject Category: Chemicals and Chemical Groups
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    , explantsexplantsSubject Category: Miscellaneous
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    , growthgrowthSubject Category: Natural Processes
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    , in vitro culturein vitro cultureSubject Category: Techniques, Methodologies and Equipment
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    , in vitro productionin vitro productionSubject Category: Techniques, Methodologies and Equipment
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    , inoculum densityinoculum densitySubject Category: Properties
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    , medicinal plantsmedicinal plantsSubject Category: Commodities and Products
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    , oxidative stressoxidative stressSubject Category: Miscellaneous
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    , plant developmentplant developmentSubject Category: Natural Processes
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    , secondary metabolitessecondary metabolitesSubject Category: Chemicals and Chemical Groups
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    , shootsshootsSubject Category: Anatomical and Morphological Structures
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    , tissue culturetissue cultureSubject Category: Techniques, Methodologies and Equipment
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    , ventilationventilationSubject Category: Techniques, Methodologies and Equipment
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    Identifier(s) : drug plants, medicinal herbs, officinal plants

    Broader term(s) : DigitalisdigitalisSubject Category: Organism Names
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    , ScrophulariaceaescrophulariaceaeSubject Category: Organism Names
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    , LamialeslamialesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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