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  • Cooled semen for fixed-time artificial insemination in beef cattle.

    Author(s) : Borges-Silva, J. C.Silva, M. R.Marinho, D. B.Nogueira, E.Sampaio, D. C.Oliveira, L. O. F.Abreu, U. G. P.Mourão, G. B.Sartori, R.

    Author Affiliation : University of São Paulo, 13418-900, Piracicaba, Brazil.

    Author Email : juliana.correa@embrapa.br

    Journal article : Reproduction, Fertility and Development 2016 Vol.28 No.7 pp.1004-1008 ref.38

    Abstract : This study evaluated the use of cooled semen in a fixed-time artificial inseminationartificial inseminationSubject Category: Techniques, Methodologies and Equipment
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    (FTAI) program compared with frozen-thawed semen to improve pregnancy rates in beef cattlebeef cattleSubject Category: Organism Groups
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    . Ejaculates of three bulls were collected and divided into two treatments: (1) frozen-thawed semen and (2) cooled semen. Egg-yolk extender without glycerol was used for the cooled semen treatment. Straws (25×106 spermatozoaspermatozoaSubject Category: Anatomical and Morphological Structures
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    ) were submitted to cooling for preservation at 5°C for 24 h, after which FTAI was performed. NeloreneloreSubject Category: Organism Names
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    neloreSubject Category: Animal Breeds
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    cows (n=838) submitted to FTAI were randomly inseminated using frozen-thawed semen or cooled semen. There was a 20% increase in the pregnancy per AI (P AI-1) using cooled semen compared with frozen-thawed semen (59.9±4.7 vs 49.4±5.0%; P<0.005). There was no difference in P AI-1 among the bulls (P=0.40). The frozen-thawed semen had fewer functional spermatozoa than did the cooled semen when evaluated by sperm motility (61.7 vs 81.0%), slow thermoresistance test (41.7 vs 66.7%) and hypoosmotic swelling test (38.3 vs 53.7%; P<0.05). The percentage of sperm abnormalities did not differ between the freeze-thawing and cooling processes (18.6 vs 22.1%; P>0.05). Because there was less damage to spermatozoa and improvement in P AI-1, the use of cooled semen instead of frozen-thawed semen is an interesting approach to increase reproductive efficiencyreproductive efficiencySubject Category: Miscellaneous
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    in cattle submitted to a FTAI protocol.

    ISSN : 1031-3613

    DOI : 10.1071/RD14185

    Record Number : 20163199687

    Publisher : CSIRO

    Location of publication : Collingwood

    Country of publication : Australia

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : cattlecattleSubject Category: Organism Names
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    , NeloreneloreSubject Category: Animal Breeds
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    Descriptor(s) : artificial inseminationartificial inseminationSubject Category: Techniques, Methodologies and Equipment
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    , beef bullsbeef bullsSubject Category: Organism Groups
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    , beef cattlebeef cattleSubject Category: Organism Groups
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    , breedsbreedsSubject Category: Organism Groups
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    , bullsbullsSubject Category: Organism Groups
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    , cattle breedscattle breedsSubject Category: Organism Groups
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    , male fertilitymale fertilitySubject Category: Miscellaneous
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    , pregnancy ratepregnancy rateSubject Category: Properties
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    , reproductive efficiencyreproductive efficiencySubject Category: Miscellaneous
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    , semen characterssemen charactersSubject Category: Properties
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    , spermatozoaspermatozoaSubject Category: Anatomical and Morphological Structures
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    Identifier(s) : AI, animal breed, animal breeds, sperm, sperm motility, sperm viability

    Broader term(s) : BosbosSubject Category: Organism Names
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    , BovidaebovidaeSubject Category: Organism Names
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    , ruminantsruminantsSubject Category: Organism Names
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    , ArtiodactylaartiodactylaSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , cattlecattleSubject Category: Organism Names
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