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  • Highly efficient synthesis of fructooligosaccharides by extracellular fructooligosaccharide-producing enzymes and immobilized cells of Aspergillus aculeatus M105 and Purification and biochemical characterization of a fructosyltransferase from the fungus.

    Author(s) : Huang MeiPingWu MinXu QiangShengMo DeJiaoFeng JiaXun

    Author Affiliation : State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi Key Laboratory of Subtropical Bioresources Conservation and Utilization, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning 530004, China.

    Author Email : jiaxunfeng@sohu.com

    Journal article : Journal of Agricultural and Food Chemistry 2016 Vol.64 No.33 pp.6425-6432

    Abstract : In this work, Aspergillus aculeatusaspergillus aculeatusSubject Category: Organism Names
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    M105 was obtained to produce high extracellular fructooligosaccharide-producing enzyme activity. The maximum yieldsyieldsSubject Category: Properties
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    of fructooligosaccharides produced by its extracellular enzymes and immobilized cells were 67.54 and 65.47% (w/w), respectively. A fructosyltransferase (FTase), AaFT32A, was purified from M105. The optimal pH and temperature of AaFT32A were pH 5.0-6.0 and 65°C, respectively. The Km, Vmax, and kcat values for the transfructosylating activity of AaFT32A were 2267 mM, 1347 µmol/min/mg protein, and 1550.2 s-1, respectively, and those values for the hydrolytic activity of AaFT32A were 6.10 mM, 32.44 µmol/min/mg protein, and 37.3 s-1, respectively. The sequence of AaFT32A deduced from the cloned gene shared 99.4% identity with a FTase from Aspergillus japonicus CB05 and a fructofuranosidase from Aspergillus nigeraspergillus nigerSubject Category: Organism Names
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    and 96.5% identity with a FTase (Aspacl_37092) from A. aculeatus ATCC 16872. The fungal strainstrainSubject Category: Properties
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    and its FTase may have potential applications in the prebiotics industry.

    ISSN : 0021-8561

    DOI : 10.1021/acs.jafc.6b02115

    Record Number : 20163308092

    Publisher : American Chemical Society

    Location of publication : Washington

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Aspergillus nigeraspergillus nigerSubject Category: Organism Names
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    , Aspergillus aculeatusaspergillus aculeatusSubject Category: Organism Names
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    Descriptor(s) : characterizationcharacterizationSubject Category: Miscellaneous
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    , purificationpurificationSubject Category: Techniques, Methodologies and Equipment
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    , strainstrainSubject Category: Properties
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    , yieldsyieldsSubject Category: Properties
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    Broader term(s) : AspergillusaspergillusSubject Category: Organism Names
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    , TrichocomaceaetrichocomaceaeSubject Category: Organism Names
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    , EurotialeseurotialesSubject Category: Organism Names
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    , EurotiomyceteseurotiomycetesSubject Category: Organism Names
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    , PezizomycotinapezizomycotinaSubject Category: Organism Names
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    , AscomycotaascomycotaSubject Category: Organism Names
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    , fungifungiSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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