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  • Establishment of PCR for detection of porcine Bocavirus type 1/2.

    Author(s) : Fu PengFeiLi MengQiQiao HanYang XingWuZhang YuXu PengLiHan HaoYingZhang HongXinChen HongYing

    Author Affiliation : Zhengzhou Major Pig Disease Prevention and Control Laboratory, Zhengzhou 450002, China.

    Author Email : chhy927@163.com

    Journal article : Zhongguo Yufang Shouyi Xuebao / Chinese Journal of Preventive Veterinary Medicine 2016 Vol.38 No.7 pp.572-575 ref.15

    Abstract : Porcine BocavirusbocavirusSubject Category: Organism Names
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    (PBoV) is a newly emerging DNA virus in 2009 which was supposed to be associated with the diarrhea in piglets. To establish the assay for PBoV detection, a pair of primers were designed and synthesized according to the highly homologous regions of published PBoV1/2 sequences available in GenBank. Thus, a PCR protocol for PBoV1/2 detection was developed under the optimized conditions for the amplification. The specific fragment of 751 bp was amplified only from the template DNA of PBoV1/2, but no amplifications were found from DNAs for other pathogens such as pseudorabies virus, porcine circovirus type 2, Salmonella or E. coli. The PCR method was capable of detecting the template DNA of 34.8 copies/µL. A total of 70 clinical samples collected from several areas from Zhongmou, Kaifeng and Puyang were detected by the established PCR assay, and 10 samples were positive for PBoV1/2 (14.29%). These results indicated that the PCR method could be application in PBoV1/2 detection in the virus infected pigspigsSubject Category: Organism Names
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    .

    ISSN : 1008-0589

    URL : http://yfsy.paperopen.com/

    Record Number : 20163308771

    Publisher : Chinese Journal of Preventive Veterinary Medicine

    Location of publication : Harbin

    Country of publication : China

    Language of text : Chinese

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : BocavirusbocavirusSubject Category: Organism Names
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    , pigspigsSubject Category: Organism Names
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    , Porcine circovirus-2porcine circovirus-2Subject Category: Organism Names
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    Descriptor(s) : accuracyaccuracySubject Category: Properties
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    , diagnosisdiagnosisSubject Category: Techniques, Methodologies and Equipment
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    , diagnostic techniquesdiagnostic techniquesSubject Category: Techniques, Methodologies and Equipment
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    , DNAdnaSubject Category: Chemicals and Chemical Groups
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    , geographical distributiongeographical distributionSubject Category: Properties
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    , polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
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    , swine diseasesswine diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , viral diseasesviral diseasesSubject Category: Diseases, Disorders, and Symptoms
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    Identifier(s) : deoxyribonucleic acid, hogs, Honan, PCR, People's Republic of China, pig diseases, Porcine bocavirus, swine, viral infections

    Geographical Location(s) : ChinachinaSubject Category: Geographic Entities
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    , HenanhenanSubject Category: Geographic Entities
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    Broader term(s) : ParvovirinaeparvovirinaeSubject Category: Organism Names
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    , ParvoviridaeparvoviridaeSubject Category: Organism Names
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    , ssDNA Virusesssdna virusesSubject Category: Organism Names
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    , DNA Virusesdna virusesSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    , Sus scrofasus scrofaSubject Category: Organism Names
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    , SussusSubject Category: Organism Names
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    , SuidaesuidaeSubject Category: Organism Names
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    , SuiformessuiformesSubject Category: Organism Names
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    , ArtiodactylaartiodactylaSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , CircoviruscircovirusSubject Category: Organism Names
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    , CircoviridaecircoviridaeSubject Category: Organism Names
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    , APEC countriesapec countriesSubject Category: Institutions and Organisations
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    , Developing Countriesdeveloping countriesSubject Category: Miscellaneous
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    , East Asiaeast asiaSubject Category: Geographic Entities
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    , AsiaasiaSubject Category: Geographic Entities
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    , Central Southern Chinacentral southern chinaSubject Category: Geographic Entities
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    , ChinachinaSubject Category: Geographic Entities
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