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  • Modulation of plant growth in vivo and identification of kinase substrates using an analog-sensitive variant of CYCLIN-DEPENDENT KINASE A;1.

    Author(s) : Harashima, H.Dissmeyer, N.Hammann, P.Nomura, Y.Kramer, K.Nakagami, H.Schnittger, A.

    Author Affiliation : Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS - UPR2357, Université de Strasbourg, F-67084 Strasbourg, France.

    Author Email : arp.schnittger@uni-hamburg.de

    Journal article : BMC Plant Biology 2016 Vol.16 No.209 pp.(26 September 2016) ref.85

    Abstract : Background: Modulation of protein activity by phosphorylationphosphorylationSubject Category: Miscellaneous
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    through kinaseskinasesSubject Category: Chemicals and Chemical Groups
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    and subsequent de-phosphorylation by phosphatases is one of the most prominent cellular control mechanisms. Thus, identificationidentificationSubject Category: Techniques, Methodologies and Equipment
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    of kinase substrates is pivotal for the understanding of many - if not all - molecular biological processes. Equally, the possibility to deliberately tune kinase activity is of great value to analyze the biological process controlled by a particular kinase. Results: Here we have applied a chemical genetic approach and generated an analog-sensitive version of CDKA;1, the central cell-cycle regulator in ArabidopsisarabidopsisSubject Category: Organism Names
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    and homolog of the yeast Cdc2/CDC28 kinases. This variant could largely rescue a cdka;1 mutant and is biochemically active, albeit less than the wild type. Applying bulky kinase inhibitorsinhibitorsSubject Category: Chemicals and Chemical Groups
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    allowed the reduction of kinase activity in an organismic context in vivo and the modulation of plant growthgrowthSubject Category: Natural Processes
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    . To isolate CDK substrates, we have adopted a two-dimensional differential gel electrophoresis strategy, and searched for proteins that showed mobility changes in fluorescently labeled extractsextractsSubject Category: Miscellaneous
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    from plantsplantsSubject Category: Organism Names
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    expressing the analog-sensitive version of CDKA;1 with and without adding a bulky ATPatpSubject Category: Chemicals and Chemical Groups
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    variant. A pilot set of five proteins involved in a range of different processes could be confirmed in independent kinase assays to be phosphorylated by CDKA;1 approving the applicability of the here-developed method to identify substrates. Conclusion: The here presented generation of an analog-sensitive CDKA;1 version is functional and represent a novel tool to modulate kinase activity in vivo and identify kinase substrates. Our here performed pilot screen led to the identification of CDK targets that link cell proliferation control to sugar metabolismmetabolismSubject Category: Natural Processes
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    , prolineprolineSubject Category: Chemicals and Chemical Groups
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    proteolysisproteolysisSubject Category: Miscellaneous
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    , and glucosinolate production providing a hint how cell proliferation and growth are integrated with plant developmentplant developmentSubject Category: Natural Processes
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    and physiology.

    ISSN : 1471-2229

    URL : http://bmcplantbiol.biomedcentral.com...

    Record Number : 20163343801

    Publisher : BioMed Central Ltd

    Location of publication : London

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : ArabidopsisarabidopsisSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    Descriptor(s) : ATPatpSubject Category: Chemicals and Chemical Groups
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    , cell cyclecell cycleSubject Category: Natural Processes
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    , extractsextractsSubject Category: Miscellaneous
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    , growthgrowthSubject Category: Natural Processes
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    , identificationidentificationSubject Category: Techniques, Methodologies and Equipment
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    , inhibitorsinhibitorsSubject Category: Chemicals and Chemical Groups
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    , kinaseskinasesSubject Category: Chemicals and Chemical Groups
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    , metabolismmetabolismSubject Category: Natural Processes
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    , mitosismitosisSubject Category: Natural Processes
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    , mutantsmutantsSubject Category: Miscellaneous
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    , mutationsmutationsSubject Category: Miscellaneous
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    , phosphoric monoester hydrolasesphosphoric monoester hydrolasesSubject Category: Chemicals and Chemical Groups
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    , phosphorylationphosphorylationSubject Category: Miscellaneous
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    , plant developmentplant developmentSubject Category: Natural Processes
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    , prolineprolineSubject Category: Chemicals and Chemical Groups
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    , proteolysisproteolysisSubject Category: Miscellaneous
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    , yeastsyeastsSubject Category: Organism Groups
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    Identifier(s) : adenosine triphosphate, phosphatases

    Broader term(s) : BrassicaceaebrassicaceaeSubject Category: Organism Names
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    , BrassicalesbrassicalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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