Author(s) :
Harashima, H.
;
Dissmeyer, N.
;
Hammann, P.
;
Nomura, Y.
;
Kramer, K.
;
Nakagami, H.
;
Schnittger, A.
Author Affiliation :
Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du CNRS, IBMP-CNRS - UPR2357, Université de Strasbourg, F-67084 Strasbourg, France.
Author Email :
arp.schnittger@uni-hamburg.de
Journal article
:
BMC Plant Biology
2016
Vol.16
No.209
pp.(26 September 2016)
ref.85
Abstract :
Background: Modulation of protein activity by
phosphorylationphosphorylationSubject Category: Miscellaneous
see more details through
kinaseskinasesSubject Category: Chemicals and Chemical Groups
see more details and subsequent de-phosphorylation by phosphatases is one of the most prominent cellular control mechanisms. Thus,
identificationidentificationSubject Category: Techniques, Methodologies and Equipment
see more details of kinase substrates is pivotal for the understanding of many - if not all - molecular biological processes. Equally, the possibility to deliberately tune kinase activity is of great value to analyze the biological process controlled by a particular kinase. Results: Here we have applied a chemical genetic approach and generated an analog-sensitive version of CDKA;1, the central cell-cycle regulator in
ArabidopsisarabidopsisSubject Category: Organism Names
see more details and homolog of the yeast Cdc2/CDC28 kinases. This variant could largely rescue a
cdka;1 mutant and is biochemically active, albeit less than the wild type. Applying bulky kinase
inhibitorsinhibitorsSubject Category: Chemicals and Chemical Groups
see more details allowed the reduction of kinase activity in an organismic context in vivo and the modulation of plant
growthgrowthSubject Category: Natural Processes
see more details. To isolate CDK substrates, we have adopted a two-dimensional differential gel electrophoresis strategy, and searched for proteins that showed mobility changes in fluorescently labeled
extractsextractsSubject Category: Miscellaneous
see more details from
plantsplantsSubject Category: Organism Names
see more details expressing the analog-sensitive version of CDKA;1 with and without adding a bulky
ATPatpSubject Category: Chemicals and Chemical Groups
see more details variant. A pilot set of five proteins involved in a range of different processes could be confirmed in independent kinase assays to be phosphorylated by CDKA;1 approving the applicability of the here-developed method to identify substrates. Conclusion: The here presented generation of an analog-sensitive CDKA;1 version is functional and represent a novel tool to modulate kinase activity in vivo and identify kinase substrates. Our here performed pilot screen led to the identification of CDK targets that link cell proliferation control to sugar
metabolismmetabolismSubject Category: Natural Processes
see more details,
prolineprolineSubject Category: Chemicals and Chemical Groups
see more details proteolysisproteolysisSubject Category: Miscellaneous
see more details, and glucosinolate production providing a hint how cell proliferation and growth are integrated with
plant developmentplant developmentSubject Category: Natural Processes
see more details and physiology.
ISSN :
1471-2229
URL :
http://bmcplantbiol.biomedcentral.com...
Record Number :
20163343801
Publisher :
BioMed Central Ltd
Location of publication :
London
Country of publication :
UK
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
ArabidopsisarabidopsisSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details
Descriptor(s) :
ATPatpSubject Category: Chemicals and Chemical Groups
see more details, cell cyclecell cycleSubject Category: Natural Processes
see more details, extractsextractsSubject Category: Miscellaneous
see more details, growthgrowthSubject Category: Natural Processes
see more details, identificationidentificationSubject Category: Techniques, Methodologies and Equipment
see more details, inhibitorsinhibitorsSubject Category: Chemicals and Chemical Groups
see more details, kinaseskinasesSubject Category: Chemicals and Chemical Groups
see more details, metabolismmetabolismSubject Category: Natural Processes
see more details, mitosismitosisSubject Category: Natural Processes
see more details, mutantsmutantsSubject Category: Miscellaneous
see more details, mutationsmutationsSubject Category: Miscellaneous
see more details, phosphoric monoester hydrolasesphosphoric monoester hydrolasesSubject Category: Chemicals and Chemical Groups
see more details, phosphorylationphosphorylationSubject Category: Miscellaneous
see more details, plant developmentplant developmentSubject Category: Natural Processes
see more details, prolineprolineSubject Category: Chemicals and Chemical Groups
see more details, proteolysisproteolysisSubject Category: Miscellaneous
see more details, yeastsyeastsSubject Category: Organism Groups
see more details
Identifier(s) :
adenosine triphosphate, phosphatases
Broader term(s) :
BrassicaceaebrassicaceaeSubject Category: Organism Names
see more details, BrassicalesbrassicalesSubject Category: Organism Names
see more details, eudicotseudicotsSubject Category: Organism Names
see more details, angiospermsangiospermsSubject Category: Organism Names
see more details, SpermatophytaspermatophytaSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details