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  • Diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas.

    Author(s) : Garima DubeyBharati KollahGour, V. K.Shukla, A. K.Mohanty, S. R.

    Author Affiliation : Indian Institute of Soil Science, Nabibagh, Bhopal 462 038, India.

    Author Email : mohantywisc@gmail.com

    Journal article : 3 Biotech 2016 Vol.6 No.2 pp.257 ref.50

    Abstract : Plant-microbial interaction in rhizosphere plays vital role in shaping plant's growth and ecosystem function. Most of the rhizospheric microbial diversity studies are restricted to bacteria. In natural ecosystem, archaea also constitutes a major component of the microbial population. However, their diversity is less known compared to bacteria. Experiments were carried out to examine diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas (J. curcas). Samples were collected from three locations varying widely in the soil physico-chemical properties. Diversity was estimated by terminal restriction fragment length polymorphism (TRFLP) targeting 16S rRNA gene of bacteria and archaea. Fifteen bacterial and 17 archaeal terminal restriction fragments (TRFs) were retrieved from J. curcas rhizosphere. Bacterial indicative TRFs were Actinobacteria, Firmicutes, Acidobacteria, Verrumicrobiaceae, and Chlroflexi. Major archaeal TRFs were crenarchaeota, and euryarchaeota. In case of bacteria, relative fluorescence was low for TRF160 and high for TRF51, TRF 420. Similarly, for archaea relative fluorescence of TRF 218, and TRF 282 was low and high for TRF 278, TRF468 and TRF93. Principal component analysis (PCA) of bacterial TRFs designated PC 1 with 46.83% of variation and PC2 with 31.07% variation. Archaeal TRFs designated 90.94% of variation by PC1 and 9.05% by PC2. Simpson index varied from 0.530 to 0.880 and Shannon index from 1.462 to 3.139 for bacteria. For archaea, Simpson index varied from 0.855 to 0.897 and Shannon index varied from 3.027 to 3.155. Study concluded that rhizosphere of J. curcas constituted of diverse set of both bacteria and archaea, which might have promising plant growth promoting activities.

    ISSN : 2190-572X

    DOI : 10.1007/s13205-016-0546-z

    URL : https://link.springer.com/article/10....

    Record Number : 20173142713

    Publisher : Springer Berlin

    Location of publication : Heidelberg

    Country of publication : Germany

    Language of text : English

    Language of summary : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Acidobacteria, Actinobacteria, Archaea, Chloroflexi (phylum), Crenarchaeota, Euryarchaeota, Firmicutes, Jatropha curcas, Verrucomicrobiaceae

    Descriptor(s) : crops, fuel crops, genetic diversity, genetic markers, genetic polymorphism, restriction fragment length polymorphism, rhizosphere bacteria, ribosomal RNA, soil bacteria

    Identifier(s) : energy crops, RFLP, rRNA

    Geographical Location(s) : India, Madhya Pradesh, Uttar Pradesh

    Broader term(s) : Bacteria, prokaryotes, Archaea, Commonwealth of Nations, Developing Countries, South Asia, Asia, Jatropha, Euphorbiaceae, Malpighiales, eudicots, angiosperms, Spermatophyta, plants, eukaryotes, India, Verrucomicrobiales, Verrucomicrobiae, Verrucomicrobia