Garima Dubey; Bharati Kollah; Gour, V. K.; Shukla, A. K.; Mohanty, S. R.
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Indian Institute of Soil Science, Nabibagh, Bhopal 462 038, India.
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Plant-microbial interaction in rhizosphere plays vital role in shaping plant's growth and ecosystem function. Most of the rhizospheric microbial diversity studies are restricted to bacteria. In natural ecosystem, archaea also constitutes a major component of the microbial population. However, their diversity is less known compared to bacteria. Experiments were carried out to examine diversity of bacteria and archaea in the rhizosphere of bioenergy crop Jatropha curcas (J. curcas). Samples were collected from three locations varying widely in the soil physico-chemical properties. Diversity was estimated by terminal restriction fragment length polymorphism (TRFLP) targeting 16S rRNA gene of bacteria and archaea. Fifteen bacterial and 17 archaeal terminal restriction fragments (TRFs) were retrieved from J. curcas rhizosphere. Bacterial indicative TRFs were Actinobacteria, Firmicutes, Acidobacteria, Verrumicrobiaceae, and Chlroflexi. Major archaeal TRFs were crenarchaeota, and euryarchaeota. In case of bacteria, relative fluorescence was low for TRF160 and high for TRF51, TRF 420. Similarly, for archaea relative fluorescence of TRF 218, and TRF 282 was low and high for TRF 278, TRF468 and TRF93. Principal component analysis (PCA) of bacterial TRFs designated PC 1 with 46.83% of variation and PC2 with 31.07% variation. Archaeal TRFs designated 90.94% of variation by PC1 and 9.05% by PC2. Simpson index varied from 0.530 to 0.880 and Shannon index from 1.462 to 3.139 for bacteria. For archaea, Simpson index varied from 0.855 to 0.897 and Shannon index varied from 3.027 to 3.155. Study concluded that rhizosphere of J. curcas constituted of diverse set of both bacteria and archaea, which might have promising plant growth promoting activities.
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Organism descriptor(s) :
Acidobacteria, Actinobacteria, Archaea, Chloroflexi (phylum), Crenarchaeota, Euryarchaeota, Firmicutes, Jatropha curcas, Verrucomicrobiaceae
crops, fuel crops, genetic diversity, genetic markers, genetic polymorphism, restriction fragment length polymorphism, rhizosphere bacteria, ribosomal RNA, soil bacteria
energy crops, RFLP, rRNA
Geographical Location(s) :
India, Madhya Pradesh, Uttar Pradesh
Broader term(s) :
Bacteria, prokaryotes, Archaea, Commonwealth of Nations, Developing Countries, South Asia, Asia, Jatropha, Euphorbiaceae, Malpighiales, eudicots, angiosperms, Spermatophyta, plants, eukaryotes, India, Verrucomicrobiales, Verrucomicrobiae, Verrucomicrobia