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  • A genome scale model of Geobacillus thermoglucosidasius (C56-YS93) reveals its biotechnological potential on rice straw hydrolysate.

    Author(s) : Ahmad AhmadHartman, H. B.Krishnakumar, S.Fell, D. A.Poolman, M. G.Shireesh Srivastava

    Author Affiliation : DBT-ICGEB Center for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110 067 New Delhi, India.

    Author Email : ahmadbioinfo@gmail.comHassan.Hartman@phe.gov.ukskrishnabio@gmail.comdfell@brookes.ac.ukmgpoolman@brookes.ac.ukshireesh@icgeb.res.in

    Journal article : Journal of Biotechnology 2017 Vol.251 pp.30-37 ref.many

    Abstract : Rice strawrice strawSubject Category: Commodities and Products
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    is a major crop residue which is burnt in many countries, creating significant air pollution. Thus, alternative routes for disposal of rice straw are needed. Biotechnological treatment of rice straw hydrolysate has potential to convert this agriculture waste into valuable biofuel(s) and platform chemicals. Geobacillus thermoglucosidasiusgeobacillus thermoglucosidasiusSubject Category: Organism Names
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    is a thermophile with properties specially suited for use as a biocatalyst in lignocellulosic bioprocesses, such as high optimal temperature and tolerance to high levels of ethanol. However, the capabilities of G. thermoglucosidasius to utilise sugars in rice straw hydrolysate for making bioethanol and other platform chemicals have not been fully explored. In this work, we have created a genome scale metabolic model (denoted iGT736) of the organism containing 736 gene products, 1159 reactions and 1163 metabolites. The model was validated both by purely theoretical approaches and by comparing the behaviour of the model to previously published experimental results. The model was then used to determine the yields of a variety of platform chemicals from glucoseglucoseSubject Category: Chemicals and Chemical Groups
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    and xylosexyloseSubject Category: Chemicals and Chemical Groups
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    - two primary sugars in rice straw hydrolysate. A comparison with results from a model of Escherichia coliescherichia coliSubject Category: Organism Names
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    shows that G. thermoglucosidasius is capable of producing a wider range of products, and that for the products also produced by E. coli, the yields are comparable. We also discuss strategies to utilise arabinose, a minor component of rice straw hydrolysate, and propose additional reactions to lead to the synthesis of xylitolxylitolSubject Category: Chemicals and Chemical Groups
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    , not currently produced by G. thermoglucosidasius. Our results provide additional motivation for the current exploration of the industrial potential of G. thermoglucosidasius and we make our model publicly available to aid the development of metabolic engineering strategies for this organism.

    ISSN : 0168-1656

    DOI : 10.1016/j.jbiotec.2017.03.031

    Record Number : 20173217829

    Publisher : Elsevier B. V.

    Location of publication : Amsterdam

    Country of publication : Netherlands

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : Escherichia coliescherichia coliSubject Category: Organism Names
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    , Geobacillus thermoglucosidasiusgeobacillus thermoglucosidasiusSubject Category: Organism Names
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    , Oryza sativaoryza sativaSubject Category: Organism Names
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    Descriptor(s) : bioenergybioenergySubject Category: Miscellaneous
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    , biofuelsbiofuelsSubject Category: Chemicals and Chemical Groups
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    , genomesgenomesSubject Category: Miscellaneous
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    , genomicsgenomicsSubject Category: Disciplines, Occupations and Industries
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    , glucoseglucoseSubject Category: Chemicals and Chemical Groups
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    , hydrolysateshydrolysatesSubject Category: Commodities and Products
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    , renewable resourcesrenewable resourcesSubject Category: Miscellaneous
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    , ricericeSubject Category: Commodities and Products
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    , rice strawrice strawSubject Category: Commodities and Products
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    , strawstrawSubject Category: Commodities and Products
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    , xylitolxylitolSubject Category: Chemicals and Chemical Groups
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    , xylosexyloseSubject Category: Chemicals and Chemical Groups
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    Identifier(s) : dextrose, E. coli, paddy, wood sugar

    Broader term(s) : EscherichiaescherichiaSubject Category: Organism Names
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    , EnterobacteriaceaeenterobacteriaceaeSubject Category: Organism Names
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    , EnterobacterialesenterobacterialesSubject Category: Organism Names
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    , GammaproteobacteriagammaproteobacteriaSubject Category: Organism Names
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    , ProteobacteriaproteobacteriaSubject Category: Organism Names
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    , BacteriabacteriaSubject Category: Organism Names
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    , prokaryotesprokaryotesSubject Category: Organism Names
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    , GeobacillusgeobacillusSubject Category: Organism Names
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    , BacillaceaebacillaceaeSubject Category: Organism Names
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    , BacillalesbacillalesSubject Category: Organism Names
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    , BacillibacilliSubject Category: Organism Names
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    , FirmicutesfirmicutesSubject Category: Organism Names
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    , OryzaoryzaSubject Category: Organism Names
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    , PoaceaepoaceaeSubject Category: Organism Names
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    , PoalespoalesSubject Category: Organism Names
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    , commelinidscommelinidsSubject Category: Organism Names
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    , monocotyledonsmonocotyledonsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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