Author(s) :
Ahmad Ahmad
;
Hartman, H. B.
;
Krishnakumar, S.
;
Fell, D. A.
;
Poolman, M. G.
;
Shireesh Srivastava
Author Affiliation :
DBT-ICGEB Center for Advanced Bioenergy Research, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, 110 067 New Delhi, India.
Author Email :
ahmadbioinfo@gmail.com
,
Hassan.Hartman@phe.gov.uk
,
skrishnabio@gmail.com
,
dfell@brookes.ac.uk
,
mgpoolman@brookes.ac.uk
,
shireesh@icgeb.res.in
Journal article
:
Journal of Biotechnology
2017
Vol.251
pp.30-37
ref.many
Abstract :
Rice strawrice strawSubject Category: Commodities and Products
see more details is a major crop residue which is burnt in many countries, creating significant air pollution. Thus, alternative routes for disposal of rice straw are needed. Biotechnological treatment of rice straw hydrolysate has potential to convert this agriculture waste into valuable biofuel(s) and platform chemicals.
Geobacillus thermoglucosidasiusgeobacillus thermoglucosidasiusSubject Category: Organism Names
see more details is a thermophile with properties specially suited for use as a biocatalyst in lignocellulosic bioprocesses, such as high optimal temperature and tolerance to high levels of ethanol. However, the capabilities of
G. thermoglucosidasius to utilise sugars in rice straw hydrolysate for making bioethanol and other platform chemicals have not been fully explored. In this work, we have created a genome scale metabolic model (denoted iGT736) of the organism containing 736 gene products, 1159 reactions and 1163 metabolites. The model was validated both by purely theoretical approaches and by comparing the behaviour of the model to previously published experimental results. The model was then used to determine the yields of a variety of platform chemicals from
glucoseglucoseSubject Category: Chemicals and Chemical Groups
see more details and
xylosexyloseSubject Category: Chemicals and Chemical Groups
see more details - two primary sugars in rice straw hydrolysate. A comparison with results from a model of
Escherichia coliescherichia coliSubject Category: Organism Names
see more details shows that
G. thermoglucosidasius is capable of producing a wider range of products, and that for the products also produced by
E. coli, the yields are comparable. We also discuss strategies to utilise arabinose, a minor component of rice straw hydrolysate, and propose additional reactions to lead to the synthesis of
xylitolxylitolSubject Category: Chemicals and Chemical Groups
see more details, not currently produced by
G. thermoglucosidasius. Our results provide additional motivation for the current exploration of the industrial potential of
G. thermoglucosidasius and we make our model publicly available to aid the development of metabolic engineering strategies for this organism.
ISSN :
0168-1656
DOI :
10.1016/j.jbiotec.2017.03.031
Record Number :
20173217829
Publisher :
Elsevier B. V.
Location of publication :
Amsterdam
Country of publication :
Netherlands
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
Escherichia coliescherichia coliSubject Category: Organism Names
see more details, Geobacillus thermoglucosidasiusgeobacillus thermoglucosidasiusSubject Category: Organism Names
see more details, Oryza sativaoryza sativaSubject Category: Organism Names
see more details
Descriptor(s) :
bioenergybioenergySubject Category: Miscellaneous
see more details, biofuelsbiofuelsSubject Category: Chemicals and Chemical Groups
see more details, genomesgenomesSubject Category: Miscellaneous
see more details, genomicsgenomicsSubject Category: Disciplines, Occupations and Industries
see more details, glucoseglucoseSubject Category: Chemicals and Chemical Groups
see more details, hydrolysateshydrolysatesSubject Category: Commodities and Products
see more details, renewable resourcesrenewable resourcesSubject Category: Miscellaneous
see more details, ricericeSubject Category: Commodities and Products
see more details, rice strawrice strawSubject Category: Commodities and Products
see more details, strawstrawSubject Category: Commodities and Products
see more details, xylitolxylitolSubject Category: Chemicals and Chemical Groups
see more details, xylosexyloseSubject Category: Chemicals and Chemical Groups
see more details
Identifier(s) :
dextrose, E. coli, paddy, wood sugar
Broader term(s) :
EscherichiaescherichiaSubject Category: Organism Names
see more details, EnterobacteriaceaeenterobacteriaceaeSubject Category: Organism Names
see more details, EnterobacterialesenterobacterialesSubject Category: Organism Names
see more details, GammaproteobacteriagammaproteobacteriaSubject Category: Organism Names
see more details, ProteobacteriaproteobacteriaSubject Category: Organism Names
see more details, BacteriabacteriaSubject Category: Organism Names
see more details, prokaryotesprokaryotesSubject Category: Organism Names
see more details, GeobacillusgeobacillusSubject Category: Organism Names
see more details, BacillaceaebacillaceaeSubject Category: Organism Names
see more details, BacillalesbacillalesSubject Category: Organism Names
see more details, BacillibacilliSubject Category: Organism Names
see more details, FirmicutesfirmicutesSubject Category: Organism Names
see more details, OryzaoryzaSubject Category: Organism Names
see more details, PoaceaepoaceaeSubject Category: Organism Names
see more details, PoalespoalesSubject Category: Organism Names
see more details, commelinidscommelinidsSubject Category: Organism Names
see more details, monocotyledonsmonocotyledonsSubject Category: Organism Names
see more details, angiospermsangiospermsSubject Category: Organism Names
see more details, SpermatophytaspermatophytaSubject Category: Organism Names
see more details, plantsplantsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details