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  • ELISA detection of vitellogenin in the haemolymph of Penaeus monodon (Fabricius, 1798) (Caridea: Penaeidae): indirect indicator of female maturity.

    Author(s) : Vrinda SukumaranSeena JoseChekidhenkuzhiyil, J.Lalithabai, M. D.Nair, S. B.Devassy ChristoKizhakedath, V. K.Paran, B. C.Philip, R.Sarojini, B. S. I.

    Author Affiliation : National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Cochin 682 016, India.

    Journal article : Journal of Crustacean Biology 2017 Vol.37 No.4 pp.473-480

    Abstract : Developing a non-lethal method for analysis of female reproductive maturity will be more accurate and less invasive than assessing maturity through gonad biopsybiopsySubject Category: Techniques, Methodologies and Equipment
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    . Quantification of the expression levels of vitellogenin (Vg) in haemolymphhaemolymphSubject Category: Miscellaneous
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    haemolymphSubject Category: Miscellaneous
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    is a vital tool to assess gonadal maturation as Vg is an indirect indicator of maturity. The lack of commercial antibody/antisera in India for detection of vitellogenin/vitellin (Vg/Vn) levels in haemolymph of Penaeus monodonpenaeus monodonSubject Category: Organism Names
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    (Fabricius, 1798) lead to the present study. To develop an assay, Vg/Vn subunits isolated together and purified from ovary of mature female P. monodon was used as antigen to raise polyclonal antisera in rabbit. This polyclonal antiserum (anti-PmVg/Vn) was used for developing Vg/Vn enzyme-linked immunosorbent assay (ELISAelisaSubject Category: Techniques, Methodologies and Equipment
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    ). Anti-PmVg/Vn demonstrated high specificity in detecting vitellogenin subunits present in haemolymph, vitellin subunits in ovary extract through Western blot analysis, and vitellin-like globules in oocytes by immunostaining/immunohistochemistry. The Vg/Vn ELISA developed through was validated by detecting haemolymph Vg based on gonadosomatic indexgonadosomatic indexSubject Category: Miscellaneous
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    of immature (stage 1) and sexually mature (stage 2) individuals of P. monodon. The specificity of the anti-PmVg/Vn raised was confirmed by detection of recombinant 74 kDa Vg subunit protein of P. monodon. The expressed recombinant Vg was also used to validate the ELISA. The results have demonstrated the specificity and sensitivity of anti-PmVg/Vn polyclonal antisera; an efficient means to monitor and transform the technology of induced breeding.

    ISSN : 0278-0372

    DOI : 10.1093/jcbiol/rux040

    Record Number : 20173323034

    Publisher : Oxford University Press

    Location of publication : Cary

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : DecapodadecapodaSubject Category: Organism Names
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    , Penaeus monodonpenaeus monodonSubject Category: Organism Names
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    Descriptor(s) : antibodiesantibodiesSubject Category: Chemicals and Chemical Groups
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    , aquatic animalsaquatic animalsSubject Category: Organism Groups
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    , aquatic invertebratesaquatic invertebratesSubject Category: Organism Groups
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    , aquatic organismsaquatic organismsSubject Category: Organism Groups
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    , biopsybiopsySubject Category: Techniques, Methodologies and Equipment
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    , breeding methodsbreeding methodsSubject Category: Techniques, Methodologies and Equipment
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    , diagnosisdiagnosisSubject Category: Techniques, Methodologies and Equipment
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    , diagnostic techniquesdiagnostic techniquesSubject Category: Techniques, Methodologies and Equipment
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    , ELISAelisaSubject Category: Techniques, Methodologies and Equipment
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    , gonadosomatic indexgonadosomatic indexSubject Category: Miscellaneous
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    , haemolymphhaemolymphSubject Category: Miscellaneous
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    , immune serumimmune serumSubject Category: Miscellaneous
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    , immunodiagnosisimmunodiagnosisSubject Category: Techniques, Methodologies and Equipment
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    , ovariesovariesSubject Category: Anatomical and Morphological Structures
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    , reproductionreproductionSubject Category: Miscellaneous
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    , sexual maturitysexual maturitySubject Category: Miscellaneous
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    , shellfishshellfishSubject Category: Organism Groups
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    , shrimpsshrimpsSubject Category: Organism Groups
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    , vitellogeninsvitellogeninsSubject Category: Chemicals and Chemical Groups
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    , western blottingwestern blottingSubject Category: Techniques, Methodologies and Equipment
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    Identifier(s) : antiserum, aquatic species, enzyme linked immunosorbent assay, hemolymphhemolymphSubject Category: Miscellaneous
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    , serological diagnosis

    Broader term(s) : PenaeuspenaeusSubject Category: Organism Names
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    , PenaeidaepenaeidaeSubject Category: Organism Names
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    , DecapodadecapodaSubject Category: Organism Names
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    , MalacostracamalacostracaSubject Category: Organism Names
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    , CrustaceacrustaceaSubject Category: Organism Names
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    , arthropodsarthropodsSubject Category: Organism Names
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    , invertebratesinvertebratesSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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