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  • System-level and granger network analysis of integrated proteomic and metabolomic dynamics identifies key points of grape berry development at the interface of primary and secondary metabolism.

    Author(s) : Wang LeiSun XiaoLiangWeiszmann, J.Weckwerth, W.

    Author Affiliation : Department of Ecogenomics and Systems Biology, University of Vienna, Vienna, Austria.

    Author Email : wolfram.weckwerth@univie.ac.at

    Journal article : Frontiers in Plant Science 2017 Vol.8 No.June pp.1066 ref.109

    Abstract : Grapevine is a fruit crop with worldwide economic importance. The grape berry undergoes complex biochemical changes from fruit setfruit setSubject Category: Natural Processes
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    until ripeningripeningSubject Category: Natural Processes
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    . This ripening process and production processes define the wine quality. Thus, a thorough understanding of berry ripening is crucial for the prediction of wine quality. For a systemic analysis of grape berry development we applied mass spectrometrymass spectrometrySubject Category: Techniques, Methodologies and Equipment
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    based platforms to analyse the metabolome and proteome of Early Campbell at 12 stages covering major developmental phases. Primary metabolites involved in central carbon metabolismmetabolismSubject Category: Natural Processes
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    , such as sugarssugarsSubject Category: Chemicals and Chemical Groups
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    , organic acidsorganic acidsSubject Category: Chemicals and Chemical Groups
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    and amino acidsamino acidsSubject Category: Chemicals and Chemical Groups
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    together with various bioactive secondary metabolitessecondary metabolitesSubject Category: Chemicals and Chemical Groups
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    like flavonolsflavonolsSubject Category: Chemicals and Chemical Groups
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    , flavan-3-ols and anthocyaninsanthocyaninsSubject Category: Chemicals and Chemical Groups
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    were annotated and quantified. At the same time, the proteomic analysis revealed the protein dynamics of the developing grape berries. Multivariate statistical analysisstatistical analysisSubject Category: Techniques, Methodologies and Equipment
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    of the integrated metabolomic and proteomic dataset revealed the growth trajectory and corresponding metabolites and proteins contributing most to the specific developmental process. K-means clustering analysis revealed 12 highly specific clusters of co-regulated metabolites and proteins. Granger causality network analysis allowed for the identificationidentificationSubject Category: Techniques, Methodologies and Equipment
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    of time-shift correlations between metabolite-metabolite, protein-protein and protein-metabolite pairs which is especially interesting for the understanding of developmental processes. The integration of metabolite and protein dynamics with their corresponding biochemical pathways revealed an energy-linked metabolism before veraison with high abundances of amino acids and accumulation of organic acids, followed by protein and secondary metabolite synthesis. Anthocyanins were strongly accumulated after veraison whereas other flavonoidsflavonoidsSubject Category: Chemicals and Chemical Groups
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    were in higher abundance at early developmental stagesdevelopmental stagesSubject Category: Miscellaneous
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    and decreased during the grape berry developmental processes. A comparison of the anthocyanin profile of Early Campbell to other cultivarscultivarsSubject Category: Organism Groups
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    revealed similarities to Concord grape and indicates the strong effect of genetic background on metabolic partitioning in primary and secondary metabolism.

    ISSN : 1664-462X

    DOI : 10.3389/fpls.2017.01066

    Record Number : 20173336704

    Publisher : Frontiers Media S.A.

    Location of publication : Lausanne

    Country of publication : Switzerland

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : VitisvitisSubject Category: Organism Names
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    , Vitis viniferavitis viniferaSubject Category: Organism Names
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    Descriptor(s) : amino acidsamino acidsSubject Category: Chemicals and Chemical Groups
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    , anthocyaninsanthocyaninsSubject Category: Chemicals and Chemical Groups
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    , cultivarscultivarsSubject Category: Organism Groups
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    , developmental stagesdevelopmental stagesSubject Category: Miscellaneous
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    , economic impacteconomic impactSubject Category: Miscellaneous
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    , effectseffectsSubject Category: Properties
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    , flavonoidsflavonoidsSubject Category: Chemicals and Chemical Groups
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    , flavonolsflavonolsSubject Category: Chemicals and Chemical Groups
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    , fruit setfruit setSubject Category: Natural Processes
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    , fruitsfruitsSubject Category: Anatomical and Morphological Structures
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    , functional genomicsfunctional genomicsSubject Category: Miscellaneous
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    , grapesgrapesSubject Category: Commodities and Products
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    , identificationidentificationSubject Category: Techniques, Methodologies and Equipment
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    , impactimpactSubject Category: Properties
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    , mass spectrometrymass spectrometrySubject Category: Techniques, Methodologies and Equipment
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    , metabolismmetabolismSubject Category: Natural Processes
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    , metabolitesmetabolitesSubject Category: Chemicals and Chemical Groups
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    , metabolomicsmetabolomicsSubject Category: Miscellaneous
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    , multivariate analysismultivariate analysisSubject Category: Techniques, Methodologies and Equipment
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    , organic acidsorganic acidsSubject Category: Chemicals and Chemical Groups
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    , phytochemicalsphytochemicalsSubject Category: Chemicals and Chemical Groups
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    , proteomicsproteomicsSubject Category: Disciplines, Occupations and Industries
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    , ripeningripeningSubject Category: Natural Processes
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    , secondary metabolitessecondary metabolitesSubject Category: Chemicals and Chemical Groups
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    , statistical analysisstatistical analysisSubject Category: Techniques, Methodologies and Equipment
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    , sugarssugarsSubject Category: Chemicals and Chemical Groups
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    Identifier(s) : cultivated varieties, growth phase, statistical methods

    Broader term(s) : VitaceaevitaceaeSubject Category: Organism Names
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    , VitalesvitalesSubject Category: Organism Names
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    , eudicotseudicotsSubject Category: Organism Names
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    , angiospermsangiospermsSubject Category: Organism Names
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    , SpermatophytaspermatophytaSubject Category: Organism Names
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    , plantsplantsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , VitisvitisSubject Category: Organism Names
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