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  • Identification of the upstream 4-chlorophenol biodegradation pathway using a recombinant monooxygenase from Arthrobacter chlorophenolicus A6.

    Author(s) : Cho SuYeonKwean OhSungYang JunWonCho WooYounKwak SeonYeongPark SungYoonLim YeJeeKim, H. S.

    Author Affiliation : Environmental Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea Republic.

    Author Email : hankim@konkuk.ac.kr

    Journal article : Bioresource Technology 2017 Vol.245 No.Part B pp.1800-1807 ref.40

    Abstract : This study aimed to clarify the initial 4-chlorophenol (4-CP) biodegradationbiodegradationSubject Category: Miscellaneous
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    pathway promoted by a two-component flavin-diffusible monooxygenase (TC-FDM) consisting of CphC-I and CphB contained in Arthrobacter chlorophenolicusarthrobacter chlorophenolicusSubject Category: Organism Names
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    A6 and the decompositiondecompositionSubject Category: Miscellaneous
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    function of CphC-I. The TC-FDM genesgenesSubject Category: Miscellaneous
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    were cloned from A. chlorophenolicus A6, and the corresponding enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    were overexpressed. Since CphB was expressed in an insoluble form, Fre, a flavin reductase obtained from Escherichia coli, was used. These enzymes were purified using Ni2+-NTA resin. It was confirmed that TC-FDM catalyzes the oxidationoxidationSubject Category: Miscellaneous
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    of 4-CP and the sequential conversionconversionSubject Category: Miscellaneous
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    of 4-CP to benzoquinone (BQN) → hydroquinonehydroquinoneSubject Category: Chemicals and Chemical Groups
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    (HQN) → HQL. This indicated that CphC-I exhibits substrate specificity for 4-CP, BQN, and HQN. The activity of CphC-I for 4-CP was 63.22 U/mg-protein, and the Michaelis-Menten kinetic parameters were vmax=0.21 mM/min, KM=0.19 mM, and kcat/KM=0.04 mM-1 min-1. These results would be useful for the development of a novel biochemical treatment technology for 4-CP and phenolic hydrocarbonshydrocarbonsSubject Category: Chemicals and Chemical Groups
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    .

    ISSN : 0960-8524

    DOI : 10.1016/j.biortech.2017.05.006

    Record Number : 20173350689

    Publisher : Elsevier Ltd

    Location of publication : Oxford

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : ArthrobacterarthrobacterSubject Category: Organism Names
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    , Arthrobacter chlorophenolicusarthrobacter chlorophenolicusSubject Category: Organism Names
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    , EscherichiaescherichiaSubject Category: Organism Names
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    , Escherichia coliescherichia coliSubject Category: Organism Names
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    Descriptor(s) : biodegradationbiodegradationSubject Category: Miscellaneous
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    , conversionconversionSubject Category: Miscellaneous
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    , decompositiondecompositionSubject Category: Miscellaneous
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    , enzymesenzymesSubject Category: Chemicals and Chemical Groups
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    , genesgenesSubject Category: Miscellaneous
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    , hydrocarbonshydrocarbonsSubject Category: Chemicals and Chemical Groups
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    , hydroquinonehydroquinoneSubject Category: Chemicals and Chemical Groups
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    , identificationidentificationSubject Category: Techniques, Methodologies and Equipment
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    , oxidationoxidationSubject Category: Miscellaneous
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    Identifier(s) : chlorophenol, E. coli

    Broader term(s) : MicrococcaceaemicrococcaceaeSubject Category: Organism Names
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    , MicrococcineaemicrococcineaeSubject Category: Organism Names
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    , ActinomycetalesactinomycetalesSubject Category: Organism Names
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    , ActinobacteridaeactinobacteridaeSubject Category: Organism Names
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    , ActinobacteriaactinobacteriaSubject Category: Organism Names
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    , BacteriabacteriaSubject Category: Organism Names
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    , prokaryotesprokaryotesSubject Category: Organism Names
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    , ArthrobacterarthrobacterSubject Category: Organism Names
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    , EnterobacteriaceaeenterobacteriaceaeSubject Category: Organism Names
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    , EnterobacterialesenterobacterialesSubject Category: Organism Names
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    , GammaproteobacteriagammaproteobacteriaSubject Category: Organism Names
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    , ProteobacteriaproteobacteriaSubject Category: Organism Names
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    , EscherichiaescherichiaSubject Category: Organism Names
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