Author(s) :
Zhou YiJun
;
Fukumura, K.
;
Nagata, S.
Author Affiliation :
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan.
Author Email :
shinjin@edu.k.u-tokyo.ac.jp
Journal article
:
Bioscience, Biotechnology and Biochemistry
2018
Vol.82
No.2
pp.274-284
ref.43
Abstract :
Adipokinetic hormone (AKH) regulates energy homeostasis in
insectsinsectsSubject Category: Organism Names
see more details by mobilizing lipid and carbohydrate from the
fat bodyfat bodySubject Category: Anatomical and Morphological Structures
see more details. Here, using
RNArnaSubject Category: Chemicals and Chemical Groups
see more details sequencing data, we identified cDNAs encoding
AKH (
GbAKH) and its highly homologous hormone
AKH/corazonin-related peptide (
GbACP) in the corpora cardiaca of the two-spotted cricket,
Gryllus bimaculatusgryllus bimaculatusSubject Category: Organism Names
see more details. RT-PCR revealed that
GbAKH and
GbACP are predominantly expressed in the corpora cardiaca and corpora allata, respectively. Phylogenetic analysis confirmed that the identified GbAKH and GbACP belong to the clades containing other AKHs and ACPs, respectively. Injection of synthetic GbAKH and GbACP elevated hemolymph carbohydrate and lipid levels and reduced food intake significantly. In contrast, knockdown of
GbAKH and
GbACP by RNA interference increased the food intake, although hemolymph lipid level was not altered. Collectively, this study provides evidence that ACP regulates hemolymph carbohydrate and lipid levels in cricket, possibly collaborative contribution with AKH to the maintenance of energy homeostasis.
ISSN :
0916-8451
DOI :
10.1080/09168451.2017.1422106
Record Number :
20183140496
Publisher :
Taylor & Francis
Location of publication :
Singapore
Country of publication :
Singapore
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
GryllusgryllusSubject Category: Organism Names
see more details, Gryllus bimaculatusgryllus bimaculatusSubject Category: Organism Names
see more details, insectsinsectsSubject Category: Organism Names
see more details
Descriptor(s) :
adipokinetic hormonesadipokinetic hormonesSubject Category: Chemicals and Chemical Groups
see more details, carbohydratescarbohydratesSubject Category: Chemicals and Chemical Groups
see more details, effectseffectsSubject Category: Properties
see more details, fat bodyfat bodySubject Category: Anatomical and Morphological Structures
see more details, phylogeneticsphylogeneticsSubject Category: Disciplines, Occupations and Industries
see more details, polymerase chain reactionpolymerase chain reactionSubject Category: Techniques, Methodologies and Equipment
see more details, RNArnaSubject Category: Chemicals and Chemical Groups
see more details
Identifier(s) :
adipokinetic, PCR, ribonucleic acid, saccharides
Broader term(s) :
GryllidaegryllidaeSubject Category: Organism Names
see more details, OrthopteraorthopteraSubject Category: Organism Names
see more details, insectsinsectsSubject Category: Organism Names
see more details, HexapodahexapodaSubject Category: Organism Names
see more details, arthropodsarthropodsSubject Category: Organism Names
see more details, invertebratesinvertebratesSubject Category: Organism Names
see more details, animalsanimalsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details, GryllusgryllusSubject Category: Organism Names
see more details