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  • Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditions.

    Author(s) : Baker, K. L.Thomas, P. R.Karriker, L. A.Ramirez, A.Zhang JianQiangWang ChongHoltkamp, D. J.

    Author Affiliation : Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University College of Veterinary Medicine, 1809 S Riverside Dr, Ames, Iowa, USA.

    Author Email : holtkamp@iastate.edu

    Journal article : BMC Veterinary Research 2017 Vol.13 No.372 pp.(1 December 2017) ref.24

    Abstract : Background: Since its emergence in 2013, porcine epidemic diarrhea virusporcine epidemic diarrhea virusSubject Category: Organism Names
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    (PEDV) spread rapidly throughout the country due, in part, to contaminated livestock trailers. The objective of this study was to test the efficacy of an accelerated hydrogen peroxidehydrogen peroxideSubject Category: Chemicals and Chemical Groups
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    (AHP) disinfectant for inactivating PEDV in swine fecesfecesSubject Category: Miscellaneous
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    fecesSubject Category: Miscellaneous
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    on metal surfaces under freezingfreezingSubject Category: Techniques, Methodologies and Equipment
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    conditions. One 15.24×15.24×2.54 cm aluminumaluminumSubject Category: Chemicals and Chemical Groups
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    aluminumSubject Category: Chemicals and Chemical Groups
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    coupon, contaminated with swine feces, and randomly matched to one pig was the experimental unit. Eight treatment groups representing two AHP concentrations (1:16 and 1:32) in a 10% propylene glycolpropylene glycolSubject Category: Chemicals and Chemical Groups
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    solution, two contact times in a -10°C freezer (40 min and 60 min), and two levels of fecal contaminationcontaminationSubject Category: Miscellaneous
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    (5 mL and 10 mL) in addition to negative and positive control groups were evaluated. Forty 3-week-old pigspigsSubject Category: Organism Names
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    , intragastrically inoculated with the contents of the coupons after treatment, were used as a bioassay to determine the infectivityinfectivitySubject Category: Properties
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    of PEDV after treatment. Infectivity was determined by detectiondetectionSubject Category: Techniques, Methodologies and Equipment
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    of virus with a nucleocapsid (N) gene-based quantitative real-time reverse transcriptionreverse transcriptionSubject Category: Natural Processes
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    polymerase chain reaction (RT-qPCR) on rectal swabs collected from the inoculated pigs on days three and seven post-inoculation. Results: All post-treatment swabs from the negative control coupons were negative for PEDV via RT-qPCR. All posttreatment swabs collected from coupons in the AHP disinfectant treatment groups and the positive control group were positive for PEDV via RT-qPCR. For the bioassay, no rectal swabs from pigs in the negative control (0 of 4) or the AHP disinfectant treatment groups (0 of 32) were positive for PEDV. Rectal swabs from all pigs within the positive control group (4 of 4) were positive for PEDV by RT-qPCR. Conclusions: Under the conditions of this study, 1:16 and 1:32 dilutions of the AHP disinfectant successfully inactivated PEDV in swine feces on metal surfaces when applied at -10°C with 40 or 60 min of contact time. This study also suggests that a positive RT-qPCR result for PEDV on an environmental sample should be expected when the AHP disinfectant is applied under freezing conditions, but does not necessarily indicate that an infectious dose of PEDV remains after disinfectiondisinfectionSubject Category: Miscellaneous
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    .

    ISSN : 1746-6148

    DOI : 10.1186/s12917-017-1300-4

    Record Number : 20183140949

    Publisher : BioMed Central Ltd

    Location of publication : London

    Country of publication : UK

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : pigspigsSubject Category: Organism Names
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    , Porcine epidemic diarrhea virusporcine epidemic diarrhea virusSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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    Descriptor(s) : aluminiumaluminiumSubject Category: Chemicals and Chemical Groups
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    , contaminationcontaminationSubject Category: Miscellaneous
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    , detectiondetectionSubject Category: Techniques, Methodologies and Equipment
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    , diarrhoeadiarrhoeaSubject Category: Diseases, Disorders, and Symptoms
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    , disinfectantsdisinfectantsSubject Category: Chemicals and Chemical Groups
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    , disinfectiondisinfectionSubject Category: Miscellaneous
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    , domestic animalsdomestic animalsSubject Category: Organism Groups
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    , epidemicsepidemicsSubject Category: Miscellaneous
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    , evaluationevaluationSubject Category: Techniques, Methodologies and Equipment
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    , faecesfaecesSubject Category: Miscellaneous
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    , freezingfreezingSubject Category: Techniques, Methodologies and Equipment
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    , hydrogenhydrogenSubject Category: Chemicals and Chemical Groups
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    , hydrogen peroxidehydrogen peroxideSubject Category: Chemicals and Chemical Groups
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    , infectious diseasesinfectious diseasesSubject Category: Diseases, Disorders, and Symptoms
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    , infectivityinfectivitySubject Category: Properties
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    , livestocklivestockSubject Category: Organism Groups
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    , livestock transporterslivestock transportersSubject Category: Techniques, Methodologies and Equipment
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    , nucleocapsid proteinsnucleocapsid proteinsSubject Category: Chemicals and Chemical Groups
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    , propylene glycolpropylene glycolSubject Category: Chemicals and Chemical Groups
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    , rectumrectumSubject Category: Anatomical and Morphological Structures
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    , reverse transcriptionreverse transcriptionSubject Category: Natural Processes
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    , techniquestechniquesSubject Category: Techniques, Methodologies and Equipment
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    , temperaturetemperatureSubject Category: Properties
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    , transcriptiontranscriptionSubject Category: Natural Processes
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    , transport of animalstransport of animalsSubject Category: Miscellaneous
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    , viral diseasesviral diseasesSubject Category: Diseases, Disorders, and Symptoms
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    Identifier(s) : aluminumaluminumSubject Category: Chemicals and Chemical Groups
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    , communicable diseases, diarrheadiarrheaSubject Category: Diseases, Disorders, and Symptoms
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    , DNA transcription, fecesfecesSubject Category: Miscellaneous
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    , hogs, livestock trailers, Porcine epidemic diarrhoea virus, scouring, swine, viral infections

    Broader term(s) : Sus scrofasus scrofaSubject Category: Organism Names
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    , SussusSubject Category: Organism Names
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    , SuidaesuidaeSubject Category: Organism Names
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    , SuiformessuiformesSubject Category: Organism Names
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    , ArtiodactylaartiodactylaSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , AlphacoronavirusalphacoronavirusSubject Category: Organism Names
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    , CoronavirinaecoronavirinaeSubject Category: Organism Names
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    , CoronaviridaecoronaviridaeSubject Category: Organism Names
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    , NidoviralesnidoviralesSubject Category: Organism Names
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    , positive-sense ssRNA Virusespositive-sense ssrna virusesSubject Category: Organism Names
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    , ssRNA Virusesssrna virusesSubject Category: Organism Names
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    , RNA Virusesrna virusesSubject Category: Organism Names
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    , virusesvirusesSubject Category: Organism Names
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