Author(s) :
Baker, K. L.
;
Thomas, P. R.
;
Karriker, L. A.
;
Ramirez, A.
;
Zhang JianQiang
;
Wang Chong
;
Holtkamp, D. J.
Author Affiliation :
Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University College of Veterinary Medicine, 1809 S Riverside Dr, Ames, Iowa, USA.
Author Email :
holtkamp@iastate.edu
Journal article
:
BMC Veterinary Research
2017
Vol.13
No.372
pp.(1 December 2017)
ref.24
Abstract :
Background: Since its emergence in 2013,
porcine epidemic diarrhea virusporcine epidemic diarrhea virusSubject Category: Organism Names
see more details (PEDV) spread rapidly throughout the country due, in part, to contaminated livestock trailers. The objective of this study was to test the efficacy of an accelerated
hydrogen peroxidehydrogen peroxideSubject Category: Chemicals and Chemical Groups
see more details (AHP) disinfectant for inactivating PEDV in swine
fecesfecesSubject Category: Miscellaneous
see more detailsfecesSubject Category: Miscellaneous
see more details on metal surfaces under
freezingfreezingSubject Category: Techniques, Methodologies and Equipment
see more details conditions. One 15.24×15.24×2.54 cm
aluminumaluminumSubject Category: Chemicals and Chemical Groups
see more detailsaluminumSubject Category: Chemicals and Chemical Groups
see more details coupon, contaminated with swine feces, and randomly matched to one pig was the experimental unit. Eight treatment groups representing two AHP concentrations (1:16 and 1:32) in a 10%
propylene glycolpropylene glycolSubject Category: Chemicals and Chemical Groups
see more details solution, two contact times in a -10°C freezer (40 min and 60 min), and two levels of fecal
contaminationcontaminationSubject Category: Miscellaneous
see more details (5 mL and 10 mL) in addition to negative and positive control groups were evaluated. Forty 3-week-old
pigspigsSubject Category: Organism Names
see more details, intragastrically inoculated with the contents of the coupons after treatment, were used as a bioassay to determine the
infectivityinfectivitySubject Category: Properties
see more details of PEDV after treatment. Infectivity was determined by
detectiondetectionSubject Category: Techniques, Methodologies and Equipment
see more details of virus with a nucleocapsid (N) gene-based quantitative real-time
reverse transcriptionreverse transcriptionSubject Category: Natural Processes
see more details polymerase chain reaction (RT-qPCR) on rectal swabs collected from the inoculated pigs on days three and seven post-inoculation. Results: All post-treatment swabs from the negative control coupons were negative for PEDV via RT-qPCR. All posttreatment swabs collected from coupons in the AHP disinfectant treatment groups and the positive control group were positive for PEDV via RT-qPCR. For the bioassay, no rectal swabs from pigs in the negative control (0 of 4) or the AHP disinfectant treatment groups (0 of 32) were positive for PEDV. Rectal swabs from all pigs within the positive control group (4 of 4) were positive for PEDV by RT-qPCR. Conclusions: Under the conditions of this study, 1:16 and 1:32 dilutions of the AHP disinfectant successfully inactivated PEDV in swine feces on metal surfaces when applied at -10°C with 40 or 60 min of contact time. This study also suggests that a positive RT-qPCR result for PEDV on an environmental sample should be expected when the AHP disinfectant is applied under freezing conditions, but does not necessarily indicate that an infectious dose of PEDV remains after
disinfectiondisinfectionSubject Category: Miscellaneous
see more details.
ISSN :
1746-6148
DOI :
10.1186/s12917-017-1300-4
Record Number :
20183140949
Publisher :
BioMed Central Ltd
Location of publication :
London
Country of publication :
UK
Language of text :
English
Indexing terms for this abstract:
Organism descriptor(s) :
pigspigsSubject Category: Organism Names
see more details, Porcine epidemic diarrhea virusporcine epidemic diarrhea virusSubject Category: Organism Names
see more details, virusesvirusesSubject Category: Organism Names
see more details
Descriptor(s) :
aluminiumaluminiumSubject Category: Chemicals and Chemical Groups
see more details, contaminationcontaminationSubject Category: Miscellaneous
see more details, detectiondetectionSubject Category: Techniques, Methodologies and Equipment
see more details, diarrhoeadiarrhoeaSubject Category: Diseases, Disorders, and Symptoms
see more details, disinfectantsdisinfectantsSubject Category: Chemicals and Chemical Groups
see more details, disinfectiondisinfectionSubject Category: Miscellaneous
see more details, domestic animalsdomestic animalsSubject Category: Organism Groups
see more details, epidemicsepidemicsSubject Category: Miscellaneous
see more details, evaluationevaluationSubject Category: Techniques, Methodologies and Equipment
see more details, faecesfaecesSubject Category: Miscellaneous
see more details, freezingfreezingSubject Category: Techniques, Methodologies and Equipment
see more details, hydrogenhydrogenSubject Category: Chemicals and Chemical Groups
see more details, hydrogen peroxidehydrogen peroxideSubject Category: Chemicals and Chemical Groups
see more details, infectious diseasesinfectious diseasesSubject Category: Diseases, Disorders, and Symptoms
see more details, infectivityinfectivitySubject Category: Properties
see more details, livestocklivestockSubject Category: Organism Groups
see more details, livestock transporterslivestock transportersSubject Category: Techniques, Methodologies and Equipment
see more details, nucleocapsid proteinsnucleocapsid proteinsSubject Category: Chemicals and Chemical Groups
see more details, propylene glycolpropylene glycolSubject Category: Chemicals and Chemical Groups
see more details, rectumrectumSubject Category: Anatomical and Morphological Structures
see more details, reverse transcriptionreverse transcriptionSubject Category: Natural Processes
see more details, techniquestechniquesSubject Category: Techniques, Methodologies and Equipment
see more details, temperaturetemperatureSubject Category: Properties
see more details, transcriptiontranscriptionSubject Category: Natural Processes
see more details, transport of animalstransport of animalsSubject Category: Miscellaneous
see more details, viral diseasesviral diseasesSubject Category: Diseases, Disorders, and Symptoms
see more details
Identifier(s) :
aluminumaluminumSubject Category: Chemicals and Chemical Groups
see more details, communicable diseases, diarrheadiarrheaSubject Category: Diseases, Disorders, and Symptoms
see more details, DNA transcription, fecesfecesSubject Category: Miscellaneous
see more details, hogs, livestock trailers, Porcine epidemic diarrhoea virus, scouring, swine, viral infections
Broader term(s) :
Sus scrofasus scrofaSubject Category: Organism Names
see more details, SussusSubject Category: Organism Names
see more details, SuidaesuidaeSubject Category: Organism Names
see more details, SuiformessuiformesSubject Category: Organism Names
see more details, ArtiodactylaartiodactylaSubject Category: Organism Names
see more details, mammalsmammalsSubject Category: Organism Names
see more details, vertebratesvertebratesSubject Category: Organism Names
see more details, ChordatachordataSubject Category: Organism Names
see more details, animalsanimalsSubject Category: Organism Names
see more details, eukaryoteseukaryotesSubject Category: Organism Names
see more details, AlphacoronavirusalphacoronavirusSubject Category: Organism Names
see more details, CoronavirinaecoronavirinaeSubject Category: Organism Names
see more details, CoronaviridaecoronaviridaeSubject Category: Organism Names
see more details, NidoviralesnidoviralesSubject Category: Organism Names
see more details, positive-sense ssRNA Virusespositive-sense ssrna virusesSubject Category: Organism Names
see more details, ssRNA Virusesssrna virusesSubject Category: Organism Names
see more details, RNA Virusesrna virusesSubject Category: Organism Names
see more details, virusesvirusesSubject Category: Organism Names
see more details