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  • Microbial integrity of preservative-free alfaxalone in a multiple-use system for two storage conditions and three handling techniques.

    Author(s) : Whitehead, M. C.Vanetten, C. L.Jacob, M. E.Harrison, T. M.

    Author Affiliation : Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.

    Author Email : tara_harrison@ncsu.edu

    Journal article : American Journal of Veterinary Research 2018 Vol.79 No.7 pp.704-710

    Abstract : OBJECTIVE: To evaluate the microbial integrity of preservative-free cyclodextrin-based alfaxalone in a multiple-use system. SAMPLE: 22 vials of preservative-free alfaxalone. PROCEDURES: 2 storagestorageSubject Category: Miscellaneous
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    conditions (room temperaturetemperatureSubject Category: Properties
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    , 22°C; refrigerated temperature, 4°C) and 3 handlinghandlingSubject Category: Miscellaneous
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    techniquestechniquesSubject Category: Techniques, Methodologies and Equipment
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    (closed system transfer device, nonclosed dispensing pin, and manufacturer-supplied vial stopper) comprised 6 treatment groups (3 replicates/group). An aliquot (0.5 mL) was withdrawn from each vial daily for 14 days. Samples were immediately inoculated into tryptic soy broth and incubated at 36°C for 24 hours; samples were subcultured onto 5% Columbia sheepsheepSubject Category: Organism Names
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    blood agar and incubated for 48 hours. Isolated colonies were evaluated for identification. RESULTS: There was no evidence of microbial contaminationmicrobial contaminationSubject Category: Miscellaneous
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    of vials stored for 7 days in refrigerationrefrigerationSubject Category: Techniques, Methodologies and Equipment
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    and handled with a protected port (closed system transfer device or nonclosed dispensing pin). CONCLUSIONS AND CLINICAL RELEVANCE: The US FDA prohibits the use of alfaxalone beyond 6 hours after the vial stopper is broached (punctured), as mandated for a preservative-free injectable medication. Findings for the study reported here supported the use of alfaxalone for 7 days when refrigerated and handled with a single puncture of the stopper by use of a protected port (closed system transfer device or nonclosed dispensing pin). This would appear to be a practical alternative for an injectable anesthetic. It would minimize drug waste and the subsequent environmental impactenvironmental impactSubject Category: Properties
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    for disposal of unused drug and allow standardizationstandardizationSubject Category: Techniques, Methodologies and Equipment
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    of storage and handling protocols for alfaxalone use in veterinary practices across the United States.

    ISSN : 0002-9645

    DOI : 10.2460/ajvr.79.7.704

    Record Number : 20183313043

    Publisher : American Veterinary Medical Association

    Location of publication : Schaumburg

    Country of publication : USA

    Language of text : English

    Indexing terms for this abstract:

    Organism descriptor(s) : sheepsheepSubject Category: Organism Names
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    Descriptor(s) : contaminationcontaminationSubject Category: Miscellaneous
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    , environmental impactenvironmental impactSubject Category: Properties
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    , evaluationevaluationSubject Category: Techniques, Methodologies and Equipment
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    , handlinghandlingSubject Category: Miscellaneous
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    , injectable anaestheticsinjectable anaestheticsSubject Category: Chemicals and Chemical Groups
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    , microbial contaminationmicrobial contaminationSubject Category: Miscellaneous
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    , refrigerationrefrigerationSubject Category: Techniques, Methodologies and Equipment
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    , standardizationstandardizationSubject Category: Techniques, Methodologies and Equipment
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    , storagestorageSubject Category: Miscellaneous
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    , techniquestechniquesSubject Category: Techniques, Methodologies and Equipment
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    , temperaturetemperatureSubject Category: Properties
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    , veterinary practiceveterinary practiceSubject Category: Disciplines, Occupations and Industries
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    Identifier(s) : environmental effects, injectable anestheticsinjectable anestheticsSubject Category: Chemicals and Chemical Groups
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    , United States of America

    Geographical Location(s) : USAusaSubject Category: Geographic Entities
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    Broader term(s) : OvisovisSubject Category: Organism Names
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    , BovidaebovidaeSubject Category: Organism Names
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    , ruminantsruminantsSubject Category: Organism Names
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    , ArtiodactylaartiodactylaSubject Category: Organism Names
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    , mammalsmammalsSubject Category: Organism Names
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    , vertebratesvertebratesSubject Category: Organism Names
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    , ChordatachordataSubject Category: Organism Names
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    , animalsanimalsSubject Category: Organism Names
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    , eukaryoteseukaryotesSubject Category: Organism Names
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    , APEC countriesapec countriesSubject Category: Institutions and Organisations
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    , Developed Countriesdeveloped countriesSubject Category: Miscellaneous
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    , North Americanorth americaSubject Category: Geographic Entities
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    , AmericaamericaSubject Category: Geographic Entities
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    , OECD Countriesoecd countriesSubject Category: Institutions and Organisations
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